Proteome analysis on the regulation mechanism of antibiotic production

点击翻译按钮获取中文摘要

In Streptomycetes, low-molecular-weight signal compounds called γ-butyrolactone autoregulators regulate the production of secondary metabolites, such as antibiotics and anticancer drugs.) From S.virginiae we have isolated virginiae butanolide (VB) a representative γ-butyrolactone autoregulator, and have clarified that the VB signal is transmitted via binding with a specific receptor protein, which finally to the production of secondary metabolites. Although many genes should participate in the signal transduction system, only a little knowledge is available on the pathway or the regulatory system until now.In this study, we investigated the regulation mechanism of virginiamycin M and S (VM and VS) production in S.virginiae by proteome and transcriptome analysis.To identify genes involved in the regulation, transcriptional patterns of several genes were compared by RT-PCR among wild-type strain, a disruptant of barA encoding a VB receptor and a disruptant of barX encoding a newly identified multi-functional regulator. It was demonstrated that transcription of genes encoding self-resistant machineries toward VS and VM were induced by VS and VM, respectively. Detailed regulatory mechanism by VB/VS/VM was investigated. Furthermore, novel genes under control of VB-BarA were identified. Proteome analysis by means of two dimensional electrophoresis resulted in the finding of several proteins showing different expression pattern between the wild type strain and the two mutant strains. Based on these results the whole regulatory mechanism of VS/VM production will be clarified in the near future.