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Analysis of radioresistant factors in cultured Mongolian gerbil cells

培养蒙古沙鼠细胞抗辐射因素分析

基本信息

批准号：
04680209
负责人：
SUSUKI Fumio
金额：
$ 1.54万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04680209/
关键词：
Mongolian Gerbil Cultured Mammalian Cells Radiosensitivity X-ray Chromosome Aberrations DNA Repair Cell Cycle Ultraviolet Light 生存率曲線 DNA損傷修復能培養細胞哺乳動物種電離放射線

项目摘要

The Mongolian gerbil (Meriones unguiculatus) is known to be one of the most radioresistant animals. In order to analyze the mechanism of high radioresistance in this animal, we have examined the X-ray sensitivity of normal diploid cells from Mongolian gerbil embryos compared with those of cultured embryo cells obtained from different mammalian species. The D0 value determined from X-ray-does survival curves for Mongolian gerbil cells ranged from 2.1 to 2.3 Gy, values which are twice as high as those for human cells. When cells were irradiated with 2 Gy of X-rays, three times longer mitotic delay was observed in human cells than in Mongolian gerbil cells. At this X-ray does, furthermore, ten times more chromosome aberrations were detected in human cells than in Mongolian gerbil cells, and frequencies of other rodent cells lay between the values for the two cell strains. Similar wide variations were observed in X-ray-does survival curves when the cell were irradiated with X-ray at passage 30 and 60 on successive transfer in culture. Interestingly, the Mongolian gerbil cells also showed a high resistance to ultraviolet light (UV), but there was no correlation between UV sensitivity of these cell strains and their DNA repair abilities. Using a pulse-field electrophoresis technique, we measured X-ray induced DNA double-strand breaks (DNA dbs) and their repair kinetics in Mongolian gerbil cells compared with those in other mammalian cells. However, a consistent kinetics of DNA dbs rejoining was observed among various mammalian cell strains. These data indicate that the radioresistance of Mongolian gerbil cells is associated with their genetic trains, and that their radioresistant factors may be some common molecules responsible to not only ionizing radiations but also UV or chemicals, rather than repair enzymes against specific DNA damages.

蒙古沙鼠（Meriones unguiculatus）是已知的最具辐射抗性的动物之一。为了分析这种动物的高辐射抗性的机制，我们已经检查了正常二倍体细胞从蒙古沙鼠胚胎的X射线敏感性与培养的胚胎细胞从不同的哺乳动物物种相比。从X射线剂量存活曲线确定的蒙古沙鼠细胞的D 0值范围为2.1至2.3戈伊，值是人类细胞的两倍。当细胞用2戈伊的X射线照射时，在人细胞中观察到的有丝分裂延迟是蒙古沙鼠细胞的三倍。此外，在这种X射线剂量下，在人类细胞中检测到的染色体畸变是蒙古沙鼠细胞的10倍，其他啮齿动物细胞的频率介于两种细胞株的值之间。在连续传代培养的第30代和第60代细胞经X射线照射后，X射线剂量-存活曲线也出现了类似的变化。有趣的是，蒙古沙鼠细胞也表现出对紫外线（UV）的高抗性，但这些细胞株的UV敏感性与它们的DNA修复能力之间没有相关性。利用脉冲场电泳技术，我们测量了X射线诱导的蒙古沙鼠细胞DNA双链断裂及其修复动力学，并与其他哺乳动物细胞进行了比较。然而，在各种哺乳动物细胞株中观察到DNA DBS重新连接的一致动力学。这些数据表明，蒙古沙鼠细胞的辐射抗性与其遗传序列有关，其辐射抗性因子可能是一些共同的分子，不仅负责电离辐射，紫外线或化学品，而不是针对特定的DNA损伤的修复酶。