Sequence analysis of X ray-induced and anticancer drug-induced mutations in cultured mammalian cells and peripheral blood lymphocytes
X射线诱导和抗癌药物诱导的培养哺乳动物细胞和外周血淋巴细胞突变的序列分析
基本信息
- 批准号:07670992
- 负责人:
- 金额:$ 1.47万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1996
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Anticancer drugs and environmental carcinogens sometimes cause a particular type of change in DNA base sequence. This type of change is useful to estimate the risk of cancer incidence of those who are exposed to these chemicals, as the change, if found, can be attributed to results of the exposure to these chemicals. The neocarzinostatin (NCS)-induced mutations were studied using cultured CHO cells, to determine if there is such a particular change. We found a variety of class of mutations (deletions, small deletions, frameshifts and base changes) in NCS-induced mutations. The rate of these classes of multations were quite similar to that of mutations arising spontaneously, suggesting that underlying mechanism may share to some extent in both mutations. On the other hand, a characteristic change was found in base change type of mutations in NCS-treated cells. The some of changed bases were at mutation site where there is AGC or ACT sequence, which was not found in mutations arising spontaneously. It is well known using oligonucleotide that NCS binds to DNA by intercalation so that these specific sequences are targeted. These findings would be helpful, when researchers want to identify whether or not mutations in oncogene or tumor suppressor gene in seocndary tumor are caused by NCS,an anticancer durg used for cancer treatment. The NCS-induced mutations in lymphocytes are now being underway.
抗癌药物和环境致癌物有时会引起DNA碱基序列的特定类型的变化。这种变化有助于估计接触这些化学品的人患癌症的风险,因为如果发现这种变化,可以归因于接触这些化学品的结果。使用培养的CHO细胞研究了新制癌素(NCS)诱导的突变,以确定是否存在这种特定的变化。我们在NCS诱导的突变中发现了各种类型的突变(缺失、小缺失、移码和碱基改变)。这类突变的发生率与自发突变的发生率非常相似,这表明两种突变的潜在机制可能在一定程度上相同。另一方面,在NCS处理的细胞中发现了碱基变化类型的突变的特征性变化。部分碱基的改变发生在有AGC或ACT序列的突变位点,而自发突变中未发现。众所周知,使用寡核苷酸,NCS通过嵌入结合到DNA,从而靶向这些特异性序列。这些发现将有助于研究人员确定是否由NCS(一种用于癌症治疗的抗癌杜尔格)引起的癌基因或抑癌基因突变。NCS诱导的淋巴细胞突变正在进行中。
项目成果
期刊论文数量(18)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Ikebuchi, M: "Are three forms of potentially lethal damage expressed after X irradiation by treatment with hypertonic solution in Chinese hamster V79 cells?" Radiat. Res.141. 19-27 (1995)
Ikebuchi, M:“在中国仓鼠 V79 细胞中,用高渗溶液处理 X 射线后,是否会表现出三种形式的潜在致命损伤?”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kimura,H.: "The scid mutation does not affect slowly repairing potentially lethal damage that is sensitive to 0.23 M NaCl" J.Radiat.Res.37. 247-255 (1997)
Kimura,H.:“scid 突变不会影响对 0.23 M NaCl 敏感的潜在致命损伤的缓慢修复”J.Radiat.Res.37。
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- 影响因子:0
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- 通讯作者:
Ogawa, H.et al.: "Spectrum of Co(II)-induced mutations in supF gene in E.coli" Kankyo Hen-igen Kenkyu. 16. 391-396 (1995)
Okawa, H.et al.:“大肠杆菌中 Co(II) 诱导的 suF 基因突变谱”Kankyo Hen-igen Kenkyu。
- DOI:
- 发表时间:
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- 影响因子:0
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Ikebuchi, M.et al.: "How many potentially lethal damage are there?" Housyasen Seibutsu Kenkyu. 31. 191-205 (1996)
Ikebuchi, M.等人:“有多少潜在的致命伤害?”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kimura, H.: "The scid mutation does not affect slowly repairing potentially lethal damage that is sensitive to 0.23M NaCl" J.Radiat.Res.37. 247-255 (1997)
Kimura, H.:“scid 突变不会影响对 0.23M NaCl 敏感的潜在致命损伤的缓慢修复”J.Radiat.Res.37。
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- 影响因子:0
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- 通讯作者:
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KIMURA Hiroshi其他文献
DNA修復のヌクレオソ-ム研究
DNA修复的核小体研究
- DOI:
- 发表时间:
2018 - 期刊:
- 影响因子:0
- 作者:
TASHIRO Satoshi;KIMURA Hiroshi;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;Satoshi Tashiro;田代 聡;Satoshi Tashiro;田代 聡;田代 聡;Satoshi Tashiro;田代 聡;Satoshi Tashiro;Satoshi Tashiro;Satoshi Tashiro;田代聡;田代聡;田代聡 - 通讯作者:
田代聡
医療放射線被ばくと染色体異常
医疗辐射暴露和染色体异常
- DOI:
- 发表时间:
2018 - 期刊:
- 影响因子:0
- 作者:
TASHIRO Satoshi;KIMURA Hiroshi;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;Satoshi Tashiro;田代 聡;Satoshi Tashiro;田代 聡;田代 聡;Satoshi Tashiro;田代 聡;Satoshi Tashiro;Satoshi Tashiro;Satoshi Tashiro;田代聡;田代聡;田代聡;田代聡;田代聡;田代聡 - 通讯作者:
田代聡
Mechanism of accurate DNA repair to prevent chromosome translocations
精确DNA修复防止染色体易位的机制
- DOI:
- 发表时间:
2018 - 期刊:
- 影响因子:0
- 作者:
TASHIRO Satoshi;KIMURA Hiroshi;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;Satoshi Tashiro;田代 聡;Satoshi Tashiro;田代 聡;田代 聡;Satoshi Tashiro;田代 聡;Satoshi Tashiro;Satoshi Tashiro;Satoshi Tashiro;田代聡;田代聡;田代聡;田代聡;田代聡;田代聡;Satoshi Tashiro;Satoshi Tashiro - 通讯作者:
Satoshi Tashiro
ゲノム修復核内ドメインの構造構築
基因组修复核结构域的结构构建
- DOI:
- 发表时间:
2018 - 期刊:
- 影响因子:0
- 作者:
TASHIRO Satoshi;KIMURA Hiroshi;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;田代 聡;Satoshi Tashiro;田代 聡;Satoshi Tashiro;田代 聡;田代 聡;Satoshi Tashiro;田代 聡;Satoshi Tashiro;Satoshi Tashiro;Satoshi Tashiro;田代聡;田代聡;田代聡;田代聡;田代聡;田代聡;Satoshi Tashiro;Satoshi Tashiro;田代聡;田代聡;田代聡;田代聡;Satoshi Tashiro;田代聡 - 通讯作者:
田代聡
Visual navigation system for dynamic walking of a quadruped on treadmill
四足动物在跑步机上动态行走的视觉导航系统
- DOI:
10.1299/transjsme.19-00316 - 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
KODONO Kodai;KIMURA Hiroshi - 通讯作者:
KIMURA Hiroshi
KIMURA Hiroshi的其他文献
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{{ truncateString('KIMURA Hiroshi', 18)}}的其他基金
Cell therapy against intractable EB virus infection using immunological tolerance
利用免疫耐受的细胞疗法对抗顽固性 EB 病毒感染
- 批准号:
24659493 - 财政年份:2012
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
An on-chip hepatic metabolism model for pharmacokinetic studies
用于药代动力学研究的片上肝代谢模型
- 批准号:
24710141 - 财政年份:2012
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
The decreases in hypoxic ventilatory response affect acute exacerbation in patients with respiratory failure and/or obesity hypoventilation syndrome
低氧通气反应的降低影响呼吸衰竭和/或肥胖低通气综合征患者的急性加重
- 批准号:
23591158 - 财政年份:2011
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Live cell imaging of histone methylation and inactive X chromosome dynamics
组蛋白甲基化和失活 X 染色体动力学的活细胞成像
- 批准号:
22370063 - 财政年份:2010
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Integrated "Body on a Chip" system for cell-based assay
用于细胞检测的集成“芯片体”系统
- 批准号:
22710120 - 财政年份:2010
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Science and Technology Studies to Develop the Governance for Effective Use of Nuclear Energy
科学技术研究发展有效利用核能的治理
- 批准号:
21360464 - 财政年份:2009
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Diagnosis and pathogenetic analysis using a novel identification method for EBV-infected cells
使用新型鉴定方法对 EBV 感染细胞进行诊断和发病机制分析
- 批准号:
21591384 - 财政年份:2009
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Infrared Spectra of Comets as a Barometer for Their Evolution
彗星的红外光谱作为其演化的晴雨表
- 批准号:
21340040 - 财政年份:2009
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Basic Researches to reexamine Problem Group called the Absolute and a Place, from the Perspective of an Image and a Field
从图像和场域的角度重新审视“绝对”和“场所”问题群的基础研究
- 批准号:
21520040 - 财政年份:2009
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Relative dielectric constant measurement system for natural soil in microwave region
微波区天然土壤相对介电常数测量系统
- 批准号:
20560393 - 财政年份:2008
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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Collaborative R&D
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