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STUDY OF BIODEGRADABLE MATERIALS FOR ANTI-ADHESIVE MEMBRANE IN VIVO

体内生物降解防粘膜材料的研究

基本信息

批准号：
05680762
负责人：
YAJIMA Hiroshi
金额：
$ 1.22万
依托单位：
NARA MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05680762/
关键词：
TENDON ANTI-ADHESIVE MATERIAL ADHESION GELATIN ENDOTOXIN

项目摘要

In the case of flexor tendon injury alone, adhesion can be prevented by early controlled mobilization. But immobilization for 4 weeks is required for a badly injured tendon with the tendon sheath defects. Crosslinked gelatin was developed to apply such cases as anti-adhesive membrane. In this study we observed the reactions of the membrane for 8 weeks in rats and the effects on reduction of adhesion for 4 weeks after tendon surgery in chickens.Materials and methods : Gelatin was crosslinked under the condition of vacuum, low pressure and 150ﾟC for 24 hours. It was made using two kinds of distillled water of different concentrations of endotoxin (endotoxin (+), (-)). The gelatin sheets were implanted in the back of rats subcutaneously. They were sacrificed at 2,4,7,10 and 14 days after implantation and histological study was performed. At 4,7 and 10 days postoperatively, infiltration of leukocyte was seen in around endotoxin (+) specimen. But its reaction was not seen around endotoxin (-) specimen until 14 days.Then the chickens aged 3 to 4 months were used. On the left third toe, the tendon sheath was removed, the flexor tendon was cut and the bony floor was injured. The tendon was sutured and wrapped around with the gelatin membrane. On the right toe, the tendon was sutured alone. The chickens were sacrificed at 2 and 4 weeks postoperatively. The toes were studied histologically and dynamic study was also performed. In the histological study, adhesion was eliminated effectively by using the membrane. In the dynamic study, there was no significant difference between the two groups at 2 and 4 weeks.

在仅屈肌腱损伤的情况下，可以通过早期控制活动来防止粘连。但对于严重损伤且腱鞘缺损的肌腱，需要固定4周。交联明胶是为了应用于防粘膜等场合而开发的。本研究观察了大鼠肌腱手术后8周膜的反应以及鸡肌腱手术后4周对减少粘连的影响。材料与方法：明胶在真空、低压、150℃条件下交联24小时。它是用两种不同浓度内毒素（内毒素（+）、（-））的蒸馏水制成的。将明胶片植入大鼠背部皮下。在植入后2、4、7、10和14天处死它们并进行组织学研究。术后4、7、10天，内毒素(+)标本周围可见白细胞浸润。但内毒素(-)标本周围直到14天才出现反应。然后使用3～4月龄的鸡。左第三趾肌腱鞘被切除，屈肌腱被切断，骨底受伤。将肌腱缝合并用明胶膜包裹。右脚趾的肌腱被单独缝合。术后2周和4周处死鸡。对脚趾进行了组织学研究，并进行了动态研究。在组织学研究中，使用膜有效地消除了粘连。在动态研究中，2周和4周时两组之间没有显着差异。