Experimental study for vascularized bone graft with tissue engineering technique

组织工程技术血管化骨移植的实验研究

• 批准号: 19591730
• 负责人: YAJIMA Hiroshi
• 金额: $ 2.83万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19591730/
• 关键词: 血管柄付き骨移植; 骨膜下移植; 培養人工骨; 人工骨

Skeletal bone defects with vascularized bone grafts has many advantages over non vascularized free grafts, but the availability of these grafts is extremely limited. Less of diameter in vascularized grafted bone caused several complication such as bone fracture. Cancelous bone graft from iliac bone and proximal of tibia were one of the clinical approach for prevent fractures of vascularized bone. but these methods has limitation of volume by donor site. Recently tissue engineered bone technique were developed and reported for clinical applications. Where the tissue engineered bone is placed is not well documented. The purpose of this study was to examine the histology and radiography of tissue engineered bone grafted under vascularized periosteum or over periosteum in F344 rats. Tissue engineered bone were placed in under or over periosteum of anterior tibia. In computed tomography, after 12 months, intensity of grafted bone increase to cortical level and maintain same intensity of cortical bone after 18 months. In under periosteum group, there were cortical bone structure right on the periosteum. the findings suggest that tissue engineered bone grafted under periosteum were divided to cortical bone and one of the method of increase the volume of vascuiarized grafted bone.

血管化骨移植治疗颅骨缺损较非血管化游离骨移植有许多优点，但其有效性非常有限。吻合血管的移植骨直径过小，易引起骨折等并发症。髂骨和胫骨近端松质骨移植是预防血管化骨骨折的临床方法之一。但是这些方法受供体部位的体积限制。组织工程骨技术是近年来发展起来的一种新型骨组织工程技术，并已进入临床应用阶段。组织工程骨的放置位置没有很好的记录。本研究的目的是观察组织工程骨在F344大鼠带血管骨膜下和骨膜上移植的组织学和影像学表现。将组织工程骨植入胫骨前骨膜下或骨膜上。在计算机断层扫描中，12个月后，移植骨的强度增加到皮质骨水平，并在18个月后保持与皮质骨相同的强度。骨膜下组，骨膜上有皮质骨结构。结果提示骨膜下移植的组织工程骨为皮质骨，是增加血管化移植骨体积的方法之一。