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Osteogenic capacity of vascularized periosteal graft combined with cultured mesenchymal stem cells

血管化骨膜移植物联合培养间充质干细胞的成骨能力

基本信息

批准号：
13671535
负责人：
YAJIMA Hiroshi
金额：
$ 2.62万
依托单位：
Nara Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

The purpose of our study was to analyze the osteogenic capacity of the vascularized * using an experimental model of the vascularized tibial periosteum and various cerai * in rats. In addition, we investigate the efficacy of cultured mesenchymal stem cells ******* vascularized periosteal graft model as well.Hydroxyapatite or β TCP as a scaffold was wrapped with the vascularized perio * composite was grafted under skin of thigh area. Marked bone formation was observed in both hydroxyapatite and β TCP group at 2 weeks or later. However, in the vascularized fascia-ceramics composite group (control group), no bone formation was observed histologically. Biochemical analysis using alkaline phosphatase and osteocalsin also indicate the same results. On the other hand, from in vitrostudy of osteoinduction by collagen apatite composite combined with cultured MSCs, osteoinduction potential of the collagen apatite composite with MSCs group was significantly higher than apatite only group as a control. Based on the results of our previous series, we performed in vivo study using vascularized periosteal graft model combined with cultured MSCs in mature rats. A collagen apatite composite was selected as the scaffold. In MSCs impregnated group, much more bone formation was observed than non-MSCs group. In conclusion, even in the matured rat which has little potential of osteogenesis, combained method of vascularized periosteum and cultured MSCs has high osteogenic capacity. And so we consider this combined method must be useful in the treatment of refractory non-union of the bones in human.

本研究的目的是利用大鼠带血管胫骨骨膜和各种骨的实验模型，分析带血管胫骨骨膜的成骨能力。此外，我们还研究了培养的骨髓间充质干细胞血管化骨膜移植模型的有效性，将血管化骨膜复合物包裹羟基磷灰石或β-磷酸三钙作为支架材料，移植于大腿皮肤下。羟基磷灰石组和β TCP组在2周及以后均有明显的骨形成。然而，在血管化筋膜陶瓷复合物组（对照组）中，组织学上未观察到骨形成。使用碱性磷酸酶和骨钙素的生化分析也表明相同的结果。另一方面，从体外研究胶原-磷灰石复合材料与培养的MSCs的骨诱导，胶原-磷灰石复合材料与MSCs组的骨诱导潜能显著高于作为对照的仅磷灰石组。在此基础上，我们采用带血管骨膜移植与骨髓间充质干细胞联合培养的方法，对成年大鼠进行了体内实验研究。选择胶原-磷灰石复合材料作为支架材料。骨髓间充质干细胞植入组的成骨能力明显强于未植入组。结论：即使在成骨能力较弱的成年大鼠中，带血管骨膜与MSCs联合培养的方法也具有较高的成骨能力。因此，我们认为这种联合方法在治疗人类难治性骨不连中一定是有用的。