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Osteogenic capacity of vascularized periosteal graft combined with cultured mesenchymal stem cells

血管化骨膜移植物联合培养间充质干细胞的成骨能力

基本信息

批准号：
13671535
负责人：
YAJIMA Hiroshi
金额：
$ 2.62万
依托单位：
Nara Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

The purpose of our study was to analyze the osteogenic capacity of the vascularized * using an experimental model of the vascularized tibial periosteum and various cerai * in rats. In addition, we investigate the efficacy of cultured mesenchymal stem cells ******* vascularized periosteal graft model as well.Hydroxyapatite or β TCP as a scaffold was wrapped with the vascularized perio * composite was grafted under skin of thigh area. Marked bone formation was observed in both hydroxyapatite and β TCP group at 2 weeks or later. However, in the vascularized fascia-ceramics composite group (control group), no bone formation was observed histologically. Biochemical analysis using alkaline phosphatase and osteocalsin also indicate the same results. On the other hand, from in vitrostudy of osteoinduction by collagen apatite composite combined with cultured MSCs, osteoinduction potential of the collagen apatite composite with MSCs group was significantly higher than apatite only group as a control. Based on the results of our previous series, we performed in vivo study using vascularized periosteal graft model combined with cultured MSCs in mature rats. A collagen apatite composite was selected as the scaffold. In MSCs impregnated group, much more bone formation was observed than non-MSCs group. In conclusion, even in the matured rat which has little potential of osteogenesis, combained method of vascularized periosteum and cultured MSCs has high osteogenic capacity. And so we consider this combined method must be useful in the treatment of refractory non-union of the bones in human.

本研究采用大鼠带血管的胫骨骨膜和各种神经的实验模型，分析带血管的胫骨骨膜的成骨能力。此外，我们还研究了培养间充质干细胞*******血管化骨膜移植模型的疗效。将羟基磷灰石或β TCP作为支架包裹血管化周膜*复合材料植入大腿皮下。2周后，羟基磷灰石组和β TCP组均有明显骨形成。然而，在血管化筋膜-陶瓷复合组（对照组），组织学上未观察到骨形成。碱性磷酸酶和骨钙素的生化分析也显示了相同的结果。另一方面，从胶原磷灰石复合材料联合培养的MSCs的体外成骨研究来看，胶原磷灰石复合材料联合MSCs组的成骨潜能显著高于单纯磷灰石组作为对照。在此基础上，我们采用带血管的骨膜移植模型结合培养的间充质干细胞在成熟大鼠体内进行了实验研究。选择胶原-磷灰石复合材料作为支架材料。MSCs浸渍组骨形成明显高于非MSCs组。综上所述，即使在成骨潜力不大的成熟大鼠中，血管化骨膜与培养间充质干细胞相结合的方法也具有较高的成骨能力。因此，我们认为这种联合方法在治疗人类难治性骨不连方面一定是有用的。