肝線維化過程における筋線維芽細胞の線維形成,収縮への神経支配の関与の研究

肝纤维化过程中神经支配参与肌成纤维细胞纤维化和收缩的研究

• 批准号: 05807047
• 负责人: 秋吉 英雄
• 金额: $ 0.51万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05807047/
• 关键词: 肝硬変症; 筋線維芽細胞; 神経支配; ペプチド神経

1.肝硬変症の発現と神経系・神経内分泌系の関与肝硬変症は総胆管結紮(モルモット)・四塩化炭素投与(ラット)にて作成、比較検討した。(1)総胆管結紮肝:結紮後1〜2週肝では門脈域での神経線維の一時的な減少と再生が観察され、神経細胞を多数認めた。この神経後胞の出現はAChE活性法および透過電顕でも確認したがその意義は現在検討中。筋線維芽細胞(MF:α-SMアクチン抗体+)は網状壊死領域に多数観察されたが門脈域ではほとんど認めない。ペプチド神経線維は、NPY(++),CGRP(+),VIP(+),SP(-)であった。(2)四塩化炭素投与肝:線維形成は小葉周辺帯で線維隔壁を形成。神経線維は線維隔壁内で多数観察された。MFはこの線維帯で多数認めた。電顕的にMFは脂肪滴を有し伊東細胞と類似の細胞様に思えるが、基底膜を介して血管内皮細胞の近傍に位置している。MFは時にグリソン鞘内で肥満細胞と接しても観察された。ペプチド神経線維は、NPY(+),CGRP(+),VIP(+)であった。2.線維形成過程にある線維芽細胞(F)および筋線維芽細胞(MF)へのコリン性神経支配の明確化、伊東細胞との神経支配の可能性の探索神経終末は、F・MFの細胞質と接触または近傍に位置して観察された。特にラット四塩化炭素投与肝ではMFとの接触が著明であった。電顕的に神経終末内の小胞は(1)有芯小胞(大型・小型)+無芯小胞と(2)多数の無芯小胞+小数の大型有芯小胞のグループを見た。結紮肝の網状壊死領域内では経過とともにα-SMアクチン陽性MFの出現を見たがこの細胞は伊東細胞がトランスフォームした可能性が強く、グリソン鞘内ではMFは認めずF様細胞のみであった。これらFおよびMFのVIPリセプター抗体の有無の検索を現在実験中である。

1. In patients with liver cirrhosis, it was found that the symptoms of liver cirrhosis were the endocrine system and the bile duct junction of liver cirrhosis. The main results were as follows: (1) Bile duct occlusion: at the end of the first two days after the end of the experiment, there was a small number of liver regeneration tests, and most of the brain cells were recognized. It was found that the AChE activity method confirmed through the electronic microscope that the meaning of the product is now in the market. The MF (α-SM antibody +) network is dead in the field, and most of the customers in the domain do not need to know how to do it. NPY (+), CGRP (+), VIP (+), SP (-). (2) the four carbons are injected into the liver: the formation of small carbon, the formation of small carbon and the formation of the next door. God knows that most of the people in the next door are looking at it. Most people recognize that the MF is in good condition. The "MF" fat droplet of the computer has a cell type similar to that of the cell cycle, and the basement membrane mediates the proximal position of the vascular endothelial cell. During MF, the fat cell in the sheath was connected with the fat cell to observe the temperature. NPY (+), CGRP (+), VIP (+). two。 The formation process of the cell (F) the germ cell (F), the bud cell (MF), the germ cell (F), the germ cell (F), Special attention is paid to the use of carbon tetrachloride and liver MF in contact with the liver. At the end of the computer, there are (1) cored cells (large and small) + cordless cells (2) most coreless cells + decimal cells. Results in the field of liver network death, there has been a significant increase in the possibility that there is a significant increase in the possibility of abnormal SM in the field of MF. In the liver network, there is a strong possibility that there is a significant increase in the possibility that there is a significant increase in the possibility of abnormal cell growth in the liver network.