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Investigation of intracellular Ca^<2+> level on uterine contraction in preterm labor

细胞内Ca^<2>水平对早产子宫收缩的影响研究

基本信息

批准号：
05807152
负责人：
ENDO Chikara
金额：
$ 1.22万
依托单位：
Fukushima Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05807152/
关键词：
Uterus IP_3 Ca^<2+>Oxytocin LPS Pregnancy Endotoxin 妊娠ラット

项目摘要

<AIM> It is suggested that there is a strong association between systemic and intrauterine infection and preterm labor. The mechanisms responsible for the initiation of parturition under these circumstances have still not been completely established. In this study, pregnant Wistar rats on day 17 of gestation were sacrificed 3 hours after intraperitoneal injection of lipopolysaccharide (LPS) or vehicle. The effects of LPS was studied on IP_3 production, intracellular Ca^<2+> level and spontaneous contractions by oxytocin (OXT) of isolated uterine strips from preterm pregnant rats.<RESULTS> 1. OXT significantly increased IP_3 production in both vehicle and LPS-treated rat uteri. The IP_3 production was significantly increased approximately 2 times by LPS treatment compared to control.2. (1) OXT stimulated uterine contraction and intracellular Ca^<2+> level in pregnant rat uterus. However, in Ca^<2+>-free medium, OXT increased uterine contraction but not intracellular Ca^<2+> level. (2) OXT stimulated uterine contraction and intracellular Ca^<2+> level in LPS-treated pregnant rat uterus.<CONCLUSIONS> 1. OXT increases IP_3 production in pregnant rat uterus. It was suggested that the mechanism of uterine contraction by OXT might be involved in Ca^<2+>-independent contraction. 2. LPS enhances IP_3 production by OXT in pregnant rat uterus. However, it is still unknown that LPS also enhances increasing intracellular Ca^<2+> level by OXT.

提示全身性和宫内感染与早产有很强的相关性。在这种情况下负责开始分娩的机制仍未完全确立。本研究选取妊娠第17天Wistar大鼠，腹腔注射脂多糖（LPS）或载药3小时后处死。研究了脂多糖对早产大鼠离体子宫条IP_3生成、细胞内Ca^<2+>水平及催产素自发收缩的影响。<结果> 1。OXT显著增加了lps处理大鼠子宫IP_3的产生。与对照相比，LPS处理显著提高了IP_3产量约2倍。(1) OXT刺激妊娠大鼠子宫收缩及细胞内Ca^<2+>水平。然而，在不含Ca^<2+>的培养基中，OXT增加了子宫收缩，但没有增加细胞内Ca^<2+>的水平。(2) OXT刺激lps处理的妊娠大鼠子宫收缩和细胞内Ca^<2+>水平。<结论> 1。OXT增加妊娠大鼠子宫IP_3的产生。提示OXT引起子宫收缩的机制可能与Ca^<2+>非依赖性收缩有关。2. LPS促进孕鼠子宫OXT产生IP_3。然而，目前尚不清楚LPS是否也能通过OXT增强细胞内Ca^<2+>水平。