Deveopment of PIXE for Small Biological Samples and Its Application to Study for Mechanism of Accumulation of Trace Elements

小生物样品PIXE的研制及其在微量元素积累机制研究中的应用

• 批准号: 06640773
• 负责人: IWATA Yoshihiro
• 金额: $ 1.22万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06640773/
• 关键词: PIXE; nondestructive analysis; biologial sample; small sample; diatom; multielement analysis; algae; accumulation; 甲殻類; 薄膜

A thin support film is indispensable to make irradiation targets of a small amount of biological material for trace anlyasis by PIXE.We developed a simple technique for the preparation of high quality support film. The thickness of this polyvinylformarl (PVF) film was 0.1 mm with good homogeneity. The continuous X-ray making background of spectrum was <1/20 that of conventional 10mm Maylar film. We calculated theoretical detection limits and also observed <1ng detection limits for many kinds of elements by 3MeV protons from a cyclotron. (Reference 1,2,4) The PVF film was applied for the support film for <200mug of biological materials. The reliability of the PIXE analysis using the PVF film was evaluated by analysis for biological standart materials. The elemental abundance in the small Crustacea such as Artemia salina was surveyed by the PVF PIXE.artemia salina was bred in artificial sea water and the characteristic of elemental abundance as a function of size and breeding day was described. (Reference 3) Diatom such as Pheodictylum sp. from Pacific Ocean was also bred in artificial sea water and the elemental abundance in the small Pheodictylum sp.was surveyed by PIXE.Accumulation behavior of trace elements especially for zinc was observed by multielemental analysis by PIXE.Many kinds of analytical methods for determination of inorganic elements are available, however, most of them are not suitable for in sutu analysis of biological materials. Biological and environmental science ofter require simultaneous determination of trace elements inliquid and gel samples. However, conventional analytical methodns require pretreatment of samples such as freeze drying, wet digestion, etc. By constant, vertical type in-air PIXE is powerful tool for in situ analysis because of no pretreatment. We have planned to install a new vertical type in-air PIXE and discussed the detection limits for many kinds of elements in liquid and thin film target. (Reference 5)

薄薄的支撑膜是必不可少的，即可通过Pixe制造少量生物材料的辐照靶标。我们开发了一种简单的技术来制备高质量的支持膜。该聚乙烯基形式（PVF）膜的厚度为0.1 mm，均匀性良好。频谱的连续X射线制作背景<1/20的10mm Maylar膜的频谱。我们计算了理论检测极限，还观察到了来自回旋子的3MEV质子的许多类型元素的<1ng检测限。 （参考文献1,2,4）将PVF膜用于<200mug的生物材料的支撑膜。使用PVF膜的Pixe分析的可靠性通过生物引导材料的分析进行了评估。 PVF Pixe调查了小甲壳类药物（例如Artemia salina）中的元素丰度。在人造海水中繁殖了Artemia salina，并描述了元素丰度的特征作为大小和繁殖日的函数。 （参考3）硅藻，例如pheodictylum sp。来自太平洋的海水也繁殖出在人造海水中，由小像素进行了调查的小细胞基属。痕量元素的蓄积行为尤其是通过Pixe进行多元分析的痕量元素。生物学和环境科学需要同时确定液体和凝胶样品的微量元素。但是，常规的分析方法需要预处理样品，例如冷冻干燥，湿法消化等。通过恒定的垂直类型的空气中像素，由于没有预处理，因此可以进行原位分析。我们计划安装新的垂直类型的空中像素，并讨论了液体和薄膜目标中许多元素的检测极限。 （参考5）

项目成果

岩田 吉弘: "体液および組織中微量元素含有量とその測定法‐粒子励起X線分光法‐" 日本臨牀. 54. 221-227 (1996)

Yoshihiro Iwata：“体液和组织中微量元素的含量及其测量方法-粒子激发X射线光谱”Nippon Rinbaku 54. 221-227 (1996)。

Y.IWATA,A.MURA and K.SERA: "Determination of Trace Elements in Several Kinds of Small Amount of Biological Materials by PIXE Analysis" JAPAN RADIOISOTOPE ASSOCIATION NISHINA MEMORIAL CYCLOTRON CENTER ANNUAL REPORT. 2. 87-90 (1994)

Y.IWATA、A.MURA 和 K.SERA：“通过 PIXE 分析测定几种少量生物材料中的痕量元素”日本放射性同位素协会 NISHINA 纪念回旋加速器中心年度报告。

岩田吉弘: "PIXE法による種々の微小生体試料中の微量元素の定量" NMCC共同利用研究成果報文集. 2. 87-90 (1994)

Yoshihiro Iwata：“使用 PIXE 方法定量各种显微生物样品中的微量元素”NMCC 合作研究报告集 2. 87-90 (1994)。

岩田吉弘: "体液および組織中微量元素含有量とその測定法 -粒子励起X線分光法-" 日本臨床. 54. 221-227 (1996)

Yoshihiro Iwata：“体液和组织中的微量元素含量及其测量方法-粒子激发X射线光谱法-”日本临床54。221-227（1996）

K.Ishii: "Compton Tail Background due to Sodium Element in Biological Samples" International Journal of PIXE. 4. 137-145 (1994)

K.Ishii：“生物样品中钠元素造成的康普顿尾背景”国际 PIXE 杂志。