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Studies on transposition and dispersion of tandem repeat sequences (TrsA) in rice genome

水稻基因组串联重复序列（TrsA）转座和分散的研究

基本信息

批准号：
06640795
负责人：
OHTSUBO Hisako
金额：
$ 1.34万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06640795/
关键词：
Tandem repeat sequences Subtelomere Rice Transposable elements 散在性反復配列

项目摘要

Our final goal of this project is to elucidate the dispersion mechanism of the tandem repeat sequences, TrsA,on rice chromosomes. The results we obtained in the past two years can be summarized as follows :(1) Involvement of transposition in dispersion of tandem repeat sequences (TrsA) in rice genome.We carried out PCR using a pair of primers that hybridize to TrsAs and flanking non-TrsA sequences, and obtained the results suggesting that TrsA sequences present at two independent loci, trsAl and trsA2, are flanked by long direct repeats of chromosomal sequences. These results may support the idea that the TrsA sequences have been inserted into each locus by transposition, resulting in duplication of chromosomal sequence used as target. We also found that the target sequence at trsA2 was present specifically in the indica rice chromosomes. Cloning and sequencing the target site of 440 bp at trsA2 revealed that this sequence was an LTR sequence of a novel retrotransposon repeated in rice … More chromosomes(2) Mapping of TrsA sequences on rice chromosomes by FISH.We mapped the TrsA sequences on the somatic prometaphase chromosomes of two O.sativa cultivars by using an improved FISH method. The FISH signals were detected at the distal ends of long arms of chromosome 6 and 12 in the japonica rice and at the distal ends of long arms of chromosome 5,7,8,9,10, and 11 in indica rice. In O.glaberrima, an African cultivated rice, the FISH signals were detected at the distal ends of long arms of chromosome 5,6 and 7. It has been known that the copy numbers of TrsA in O.meridionalis, a wild type rice, are almost ten times higher than those in O.sativa. FISH analysis showed that the hybridization signals were detected at the distal ends of long arms of all the chromosomes except 12. These results suggest that the numbers and locations of the TrsA on the chromosomes are characteristic to each rice species. It should be noticed that all the signals were detected at the distal ends of long arms of chromosomes except one (chromosome 4 to O.meridionalis). These results indicate that chromosomes have polarity which may play an important role in gathering chromosomes. Less

我们的最终目标是阐明串联重复序列TrsA在水稻染色体上的分散机制。（1）水稻基因组中串联重复序列（TrsA）的分布与转座的关系本研究利用一对与TrsA杂交的引物，在非TrsA序列的两侧进行PCR扩增，结果表明存在于两个独立位点（trsA1和trsA2）上的TrsA序列两侧都有染色体长的直接重复序列。这些结果可能支持这样的想法，即TrsA序列已通过转座插入到每个基因座中，导致用作靶的染色体序列的重复。我们还发现，在trsA2的目标序列特异性地存在于籼稻染色体。克隆并测序了trsA2的440 bp的目的位点，发现该序列是一个在水稻中重复的反转录转座子的LTR序列 ...更多信息（2）TrsA基因在水稻染色体上的定位利用改进的FISH方法，将TrsA基因定位在两个水稻品种的体细胞前中期染色体上。在粳稻第6、12号染色体长臂末端检测到FISH信号，在籼稻第5、7、8、9、10、11号染色体长臂末端检测到FISH信号。在非洲栽培稻O.glaberrima中，在第5、6和7号染色体长臂的远端检测到FISH信号。已知野生型水稻水稻中TrsA的拷贝数几乎是水稻中的10倍。FISH分析表明，除12号染色体外，其余染色体长臂末端均检测到杂交信号。这些结果表明，染色体上的TrsA的数量和位置是每个水稻品种所特有的。值得注意的是，除了一条染色体（4号染色体到O.dumonalis）外，所有的信号都在染色体长臂的远端检测到。这些结果表明，染色体具有极性，这可能在染色体收集中起重要作用。少