Studied on gypsy-type retrotransposons in plant ; their stuructural diversity and their choromosomal localization

植物吉普赛型反转录转座子的研究；

• 批准号: 12640596
• 负责人: OHTSUBO Hisako
• 金额: $ 2.24万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640596/
• 关键词: gypsy-type retrotransposon; tandem repeat sequence; beterochromatin; ceitromere region; rice; FISH (Fluorescent in situ Hybridization); vertical transmission; evolution; イネゲノム; レトロトランスポゾン; トランスポゾン; 縦列型反復配列; FISH法; セントロメア

Phylogenetic analysis of the rt genes of gypsy-type retrotransposons from rice (RIRE3, RIRE8 and RIRE2) as well as various plants (monocots, dicots, pine, ginkgo, horsetail, liverwort and algae) showed that these retrotransposon homologues could be classified into two families, in each of which, they were further classified into several subfamilies. This indicates that sequence divergence during vertical transmission has been a major influence on the evolution of gypsy-type retrotransposons in plants. An another rice gypsy-type retrotranson, named RIRE7, is a retrotransposon wigh homology to several DNA segaments present in the centromere regions of cereal chromosomes. RIRE7 were also found to be inserted into a site within a tandem repeat sequence with a unit length of 155bp, named TrsD. FISH analysis of the metaphase chromosomes of *. sativa showed that both RIRE7 and TrsD sequences were present in the centromere region of the chromosomes. Further FISH analysis of rice pachytene chromosomes showed that the TrsD sequences were located in a pericentromeric heterochromatin region. These findings strongly sugge that RIRE7, as well as TrsD, is an important component of the pericentromeric heterochromatin of rice chromosomes.

对水稻（RIRE3、RIRE8和RIRE2）以及多种植物（单子叶植物、双子叶植物、松树、银杏、马尾草、地钱和藻类）的吉普赛型反转录转座子的rt基因进行系统发育分析表明，这些反转录转座子同源物可分为两个家族，每个家族中又进一步分为几个亚家族。这表明垂直传播过程中的序列分歧对植物中吉普赛型反转录转座子的进化产生了主要影响。另一种水稻吉普赛型反转录转子，名为 RIRE7，是与谷物染色体着丝粒区域中存在的几个 DNA 片段同源的反转录转座子。 RIRE7还被发现插入到单位长度为155bp的串联重复序列内的位点，命名为TrsD。 * 中期染色体的 FISH 分析。苜蓿显示 RIRE7 和 TrsD 序列都存在于染色体的着丝粒区域。对水稻粗线期染色体的进一步 FISH 分析表明，TrsD 序列位于着丝粒周围异染色质区域。这些发现强烈表明 RIRE7 以及 TrsD 是水稻染色体着丝粒周围异染色质的重要组成部分。

项目成果

Cheng C, Tsuchimoto S, Ohtsubo H, and Ohtsubo E: "Tnr8, a foldback transposable element from rice"Genes genet. Syst. 75. 327-333 (2000)

Cheng C、Tschimoto S、Ohtsubo H 和 Ohtsubo E：“Tnr8，来自水稻的折返转座元件”Genes Genet。

Kumekawa N, Ohtsubo H, Horiuchi T, and Ohtsubo E: "Identification and characterization of novel retrotransposons of gypsy type in rice"Mol. Gen. Genet. 260. 593-602 (1999)

Kumekawa N、Ohtsubo H、Horiuchi T 和 Ohtsubo E：“水稻吉普赛型新型反转录转座子的鉴定和表征”Mol。

Kumekawa, N., Ohmido, N., Fukui, K., Ohtsubo, E., Ohtsubo, H.: "A new gypsy-type retrotransposon RIRE7 : preferential insertion into the tandem repeat sequence TrsD in pericentromic heterochromatin regions of rice chromosomes"Mol. Gen. Genet.. 265. 480-48

Kumekawa, N.、Ohmido, N.、Fukui, K.、Ohtsubo, E.、Ohtsubo, H.：“一种新的吉普赛型逆转录转座子 RIRE7：优先插入水稻染色体着丝粒周围异染色质区域的串联重复序列 TrsD”

Noma, K., Ohtsubo, H., Ohtsubo, E.: "A new class of LINEs (ATLN-L) from Arabidopsis thaliana with extraordinary structural features"DNA Res.. 8. 291-299 (2001)

Noma, K.、Ohtsubo, H.、Ohtsubo, E.：“来自拟南芥的具有非凡结构特征的新一类 LINE (ATLN-L)”DNA Res.. 8. 291-299 (2001)

Hen C. - G, Frank M. J, Ohtsubo H, and Ohtsubo E: "New transposable elements identified as insertions in rice transposon"Tnr1. Genes Genet. Syst. 75. 69-77 (2000)

Hen C. - G、Frank M. J、Ohtsubo H 和 Ohtsubo E：“新的转座元件被鉴定为水稻转座子中的插入”Tnr1。