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Studies of the novel retrotransposable elements appeared on rice chromosomes

对水稻染色体上出现的新型逆转录转座元件的研究

基本信息

批准号：
08640785
负责人：
OHTSUBO Hisako
金额：
$ 1.41万
依托单位：
Institute of Molecular and Cellular Bioscience
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08640785/
关键词：
rice retrotransposon Reverse transcriptase horizontal transfer vertical transfer LINE 入れ子構造

项目摘要

We have identified and characterized various retrotransposons with or without LTR in the genome of O.sativa. RIRE1, a retrotransposon of copia type, was originally found in wild rice O.austrariensis in an extraordinary number of copies. RIRE2, RIRE3 and RIRE8 were isolated from O.sativa IR36. These elements turned out to be retrotransposons of gypsy type with LTR.These elements were often nested one another on rice chromosomes. RIRE2 was originally found as a sequence associated with TrsA,a tandem repeat sequence at the subtelomeric regions of the O.sativa chromosome. The gag and the pol regions of RIRE2 show high homology with those of Grande1, a teosinte retrotransposon with LTR.The amino acid sequences of rt genes of these gypsy-type retrotransposons revealed that they were divided into two groups, such as RIRE3-RIRE8 family and RIRE2-Grande1 family. We have also identified LINE elements, non-LTR retrotransposons, from the various members of the higher plants including rice. Sequence analysis revealed that these elements contained amino acid sequence motifs conserved in the region. Phylogenetic analysis indicated that these elements were transmitted vertically unlike other retrotransposons with LTR,which were believed to be transmitted horizontally.

我们已经鉴定并表征了水稻基因组中具有或不具有 LTR 的各种反转录转座子。 RIRE1 是一种 copia 型逆转录转座子，最初在野生稻 O.austrariensis 中发现，其拷贝数非常多。 RIRE2、RIRE3 和 RIRE8 是从 O. sativa IR36 中分离出来的。这些元件被证明是具有 LTR 的吉普赛型逆转录转座子。这些元件通常彼此嵌套在水稻染色体上。 RIRE2 最初被发现是与 TrsA 相关的序列，TrsA 是水稻染色体亚端粒区域的串联重复序列。 RIRE2的gag和pol区域与LTR类大蜀黍反转录转座子Grande1具有高度同源性。这些吉普赛型反转录转座子的rt基因氨基酸序列显示它们分为两类，即RIRE3-RIRE8家族和RIRE2-Grande1家族。我们还从包括水稻在内的高等植物的各个成员中鉴定出了 LINE 元件，即非 LTR 反转录转座子。序列分析表明这些元件含有该区域保守的氨基酸序列基序。系统发育分析表明，这些元件是垂直传播的，不像其他具有LTR的反转录转座子被认为是水平传播的。