Evolution and function of Marchantia BBR/BPC proteins

地钱 BBR/BPC 蛋白的进化和功能

• 批准号: 443101600
• 负责人: Professor Dr. Dierk Wanke
• 金额: --
• 依托单位: Bereich Systematik, Biodiversität & Evolution der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/443101600?language=en
• 关键词: Evolution; function; Marchantia; BBR; BPC

This short term project addresses the question whether the molecular biochemical functions of BBR/BPC proteins were already established in the dioecious liverwort Marchantia, as one of the earliest land plants, and maintained throughout evolution, or not.Because of the conserved tripartide domain structure, it might be possible that Marchantia MpBBR1 and MpBBR2 can form homodimers and heterodimers with MpLHP1. Based on our data from the orthologous proteins in Arabidopsis, it might be possible that MpLHP1 can be recruited by MpBBR1 or MpBBR2 to GAGA-motif containing DNA-probes. The concerted action of the MpBBR-MpLHP1 complex will presumably target homeotic genes and, hence, might be important for the development of reproductive organs. Cross-species heterodimer formation between Arabidopsis LHP1 or group II BBR/BPCs and Marchantia LHP1 or group IV BBR/BPC members will disclose whether the molecular functional capacities were maintained throughout land plant evolution.Against the background of my hypotheses, I will address the following central questions:• Are MpBBR1 and MpBBR2 forming homodimers in vivo? Where do the proteins localize inside the nucleus? As a consequence of gametophytic dioecy, it is important to know whether MpBBR1 - MpBBR2 homotypic heterodimers can form in the zygote.• Are both MpBBR1 and MpBBR2 able to interact with MpLHP1? Is this interaction restricted to the nucleoplasm, similar to their Arabidopsis othologs?• Are orthologous proteins from Arabidopsis and Marchantia able to form dimers in vivo?• Are MpBBR1 and MpBBR2 binding to GAGA-DNA-motifs? If so, it is important to know whether MpLHP1 can be recruited by MpBBR1 or MpBBR2 to GAGA-motifs.• Are MpBBR1 and MpBBR2 targeting MADS-box gene promoters - similar to their Arabidopsis orthologs?

这个短期项目解决了一个问题，即BBR/BPC蛋白的分子生化功能是否已经在exiousious Liverwort Marchantia（作为最早的土地植物之一）中建立，并且在整个进化过程中都保持了。由于配置的三方结构结构，Marchantia MPBBR1和MPBBR2可能会与MPLHP1形成同型二聚体和异二聚体。基于我们从拟南芥中的直系同源蛋白的数据，MPLHP1可能会被MPBBR1或MPBBR2募集到含有DNA探针的GAGA-MOTIF。 MPBBR-MPLHP1复合物的一致作用可能会靶向同型基因，因此对于生殖器官的发展可能很重要。拟南芥LHP1或II组BBR/BPC和Marchantia LHP1或IV IV BBR/BPC成员之间的跨物种异二聚体形成，将披露分子功能能力是否在整个地面工厂进化中维持在整个整个土地工厂的演化中。蛋白质在核内部在哪里定位？由于配子型二元组合，重要的是要知道MPBBR1 -MPBBR2同型异二聚体是否可以在Zygote中形成。这种相互作用是否仅限于核等离子体，类似于它们的拟南芥植物学？如果是这样，重要的是要知道MPLHP1是否可以由MPBBR1或MPBBR2募集到Gaga-Motifs。