Mechanisms for signal transduction of meiotic pheromone in yeast

酵母减数分裂信息素信号转导机制

• 批准号: 06660427
• 负责人: YAMASHITA Ichiro
• 金额: $ 0.58万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660427/
• 关键词: Yeast; Meiosis; Pheromone; Transcriptional regulation

Diploid cells of the yeast S.cerevisiae with mating type a/alpha differentiate into meiosis when starved for nutrients. In this study, we demonstrated that social communication via meiotic pheromone (MEP) is essential for the differentiation.1. We obtained 4,000 mutant clones which do not express ime2-lacZ.From the mutant clones, 5 clones expressed the ime2-lacZ when crossed with wild-type cells. These mutants were considered to have defects especially in MEP production.2. Five mutant clones were found to have mutations in the same locus, socl (social communication). Homozygous socl diploids did not develop into meiosis, suggesting the essential role for MEP in meiosis.3. We further obtained regulatory genes (SOC2, SOC3, and SOC4) for MEP production by screening suppressor mutations of the socl-2.4. The SOC2-4 genes encode proteins regulating TCA cycle, suggesting that MEP is an organic acid.

具有交配型a/α的酿酒酵母的二倍体细胞在缺乏营养时分化成减数分裂。在这项研究中，我们证明了通过减数分裂信息素（MEP）的社会交流是必不可少的分化。我们获得了4，000个不表达ime 2-lacZ的突变克隆。从突变克隆中，5个克隆在与野生型细胞杂交时表达ime 2-lacZ。这些突变体被认为是有缺陷的，特别是在MEP生产方面.发现5个突变克隆在同一个位点socl（社会交流）发生突变。纯合socl二倍体没有发生减数分裂，提示MEP在减数分裂中起重要作用.我们通过筛选socl-2.4的抑制突变进一步获得了MEP产生的调控基因（SOC 2、SOC 3和SOC 4）。SOC 2 -4基因编码TCA循环调节蛋白，提示MEP是一种有机酸。

项目成果

山下一郎(分担): "酵母に見る最新の真核生物像" 共立出版, 790 (1994)

Ichiro Yamashita（贡献者）：“酵母中看到的真核生物的最新图片”Kyoritsu Shuppan，790（1994）

Ichiro Yamashita: "Yeast studies developing into the 21st century". Gakkai Shuppan Center, 252 (1994)

山下一郎：“酵母研究发展到21世纪”。

山下一郎(分担): "21世紀に向かう酵母研究" 学会出版センター, 252 (1994)

山下一郎（撰稿人）：《迈向21世纪的酵母研究》学会出版中心，252（1994）

Ichiro Yamashita: "The newest views of eukaryotic cells from yeast". Kyoritsu Shuppan, 790 (1994)

Ichiro Yamashita：“来自酵母的真核细胞的最新观点”。