Mechanisms for signal transduction in the initiation of meiosis in enkaryotes

核生物减数分裂启动的信号转导机制

• 批准号: 03660115
• 负责人: YAMASHITA Ichiro
• 金额: $ 1.41万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03660115/
• 关键词: Meiosis; Sporulation; Transcriptional Regulation; Protein Kinase; プロティンキナーゼ; プロティンキナ-ゼ

1. We isolated IME2, SME2, and SME3 genes as positive regulators for the initiation of meiosis. IME2 was transcribed specifically at an early stage of meiosis. Transcripts from SME2 and SME3 were accumulated in stationary cells. SME2 activated transcription of SGA1, a late meiosis-specific gene, independently of IME1 and IME2. SME3 activated IME1 transcription. GAM1 and GAM3, positive regulators for STA1, were also required for transcription of IME1. SUD1, GAM2, NIM1, and NIM2 were required for repression of IME2. From these results, we proposed a model for regulatory cascades governing the initiation of meiosis. 2. Using IME2-specific antisera, we demonstrated nuclear localization and protein kinase activity of IME2 protein. Carboxy-terminal domain of IME2 consisting of six highly acidic subpeptides appeared to have a negative role. 3. We also examined the mechanism for transcriptional regulation of SGA1 by IME2 kinase. 5' upstream region of SGA1 contained positive (UAS) and negative (NRE) elements for transcription. UAS activity was not regulated by IME2. Deletion of NRE activated transcription of SGA1 independently of IME2. These results suggest that IME2 kinase relieves negative regulation through NRE. We also identified UAS- and NRE-binding proteins by gel shift assay.

1.我们分离出IME2、SME2和SME3基因作为减数分裂起始的正调节因子。 IME2 在减数分裂的早期阶段被特异性转录。 SME2 和 SME3 的转录本在静止细胞中积累。 SME2 独立于 IME1 和 IME2 激活 SGA1（减数分裂晚期特异性基因）的转录。 SME3 激活 IME1 转录。 IME1 的转录也需要 GAM1 和 GAM3（STA1 的正调节因子）。 SUD1、GAM2、NIM1 和 NIM2 是抑制 IME2 所必需的。根据这些结果，我们提出了控制减数分裂启动的调控级联模型。 2.利用IME2特异性抗血清，我们证明了IME2蛋白的核定位和蛋白激酶活性。由六个高酸性亚肽组成的 IME2 羧基末端结构域似乎具有负面作用。 3.我们还研究了IME2激酶对SGA1转录调控的机制。 SGA1 的 5' 上游区域包含用于转录的正向 (UAS) 和负向 (NRE) 元件。 UAS 活动不受 IME2 监管。 NRE 的删除独立于 IME2 激活 SGA1 的转录。这些结果表明 IME2 激酶通过 NRE 缓解负调节。我们还通过凝胶位移测定鉴定了 UAS 和 NRE 结合蛋白。

项目成果

H.Kawaguchi: "Nutritional regulation of meiosis-specific gene expression in Saccharomyces cerevisiae" Bioscience, Biotechnology, and Biochemistry. 56. 289-297 (1992)

H.Kawaguchi：“酿酒酵母减数分裂特异性基因表达的营养调节”生物科学、生物技术和生物化学。

H. Yoshimoto: "Identity of the GAM3 qene with ADR6,each required for transcription of the STA1 or ADH2 gene in Saccharomyces cerevisiae" Bioscience,Biotechnoloqy,and Biochemistry. 56. 527-529 (1992)

H. Yoshimoto：“GAM3 基因与 ADR6 的同一性，每个基因都是酿酒酵母中 STA1 或 ADH2 基因转录所必需的”生物科学、生物技术和生物化学。

H. Yoshimoto: "Identity of the GAM3 gene with ADR6, each required for transcription of the STA1 or ADH2 gene in Saccharomyces cerevisiae" Biosci. Biotech. Biochem. 56. 527-529 (1992)

H. Yoshimoto：“GAM3 基因与 ADR6 的同一性，每个基因都是酿酒酵母中 STA1 或 ADH2 基因转录所必需的”Biosci。

H.Kawaguchi: "Nutritional regulation of meiosisーspecific gene expression in Saccharomyces cerevisiae" Biocience,Biotechnology,and Biochemistry. 56. 289-297 (1992)

H. Kawaguchi：“酿酒酵母减数分裂特异性基因表达的营养调节”《生物科学、生物技术和生物化学》56. 289-297 (1992)。

H.Yoshimoto: "The GAM1/SNF2 gene of Saccharomyces cerevisiae encodes a highly charged nuclear protein required for transcription of the STA1 gene" Molecular and General Genetics. 228. 270-280 (1991)

H.Yoshimoto：“酿酒酵母的 GAM1/SNF2 基因编码 STA1 基因转录所需的高电荷核蛋白”《分子与普通遗传学》。