Basic study on neuronal conrol of heart

心脏神经元控制的基础研究

• 批准号: 06670073
• 负责人: YAMAZAKI Toji
• 金额: $ 0.96万
• 依托单位: National cardiovascular center reseatch institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670073/
• 关键词: chromatogram; sympathetic nerves; dialysis; noradrenaline; neural control; heart; 交感神経活動

1) The present study describes a high-performance liquid chromatographic-electrochemical detection (HPLC-ECD) system for routine measurement of the low levels of norepinephrine (NE) found in the myocardial interstitial space. In this system, an in vivo detection limit of 100 fg in a 50mul injection was achieved for NE.Using cardiac dialysis technique, 20mu dialysates were sampled from the myocardial interstitial space at 2-min intervals. The basal dialysate NE concentration was 16.6<plus-minus>4.0pg/ml. This low detection limit allowed the dialysate NE concentration to be monitored for dysfunction of the cardiac sympathetic nerve terminal. This system offers a new possibility for routine analysis of myocardial interstitial NE level2) The present study describes a high-performance liquid chromatographic electrochemical detection (HPLC-ECD) system for monitoring of dihydroxyphenylglycol (DHPG) in the myocardial interstitial spsce. Using cardiac dialysis technique, 10mul dialysates were sampled from the myocardial interstitial space (1min-fraction), and directly injected to HPLC-ECD system. An in vivo detection limit of 100 fg in a 10mul injection was achieved for DHPG.The basal dialysate DHPG concentration was 181<plus-minus>46 pg/ml. This system offers a new possibility for monitoring of myocardial interstitial DHPG level

1)本研究描述了一种高效液相色谱-电化学检测（HPLC-ECD）系统，用于常规测量心肌间质间隙中低水平的去甲肾上腺素（NE）。在该系统中，NE在50毫升注射中达到100 fg的体内检出限。采用心脏透析技术，每隔2分钟从心肌间隙抽取20 μ m的透析液。基础透析液NE浓度为16.6<±bb0 4.0pg/ml。这种低检测限允许透析液NE浓度监测心脏交感神经末梢功能障碍。本研究建立了一种高效液相色谱电化学检测（HPLC-ECD）系统，用于监测心肌间质中二羟基苯基乙二醇（DHPG）的含量。采用心脏透析技术，从心肌间隙（1min-fraction）取样10个透析液，直接注射到HPLC-ECD系统。DHPG的体内检出限为100 fg。基础透析液DHPG浓度为181<正负bb0 46 pg/ml。该系统为监测心肌间质DHPG水平提供了一种新的可能性

项目成果

北川裕利、秋山剛、山崎登自、: "下大動脈閉塞時の心筋間質ノルエピネフリン動態" 心臓. 27. 56-59 (1995)

Yuri Kitakawa、Tsuyoshi Akiyama、Toji Yamazaki：“下主动脉闭塞期间心肌间质去甲肾上腺素动态”Cardiac。

T.Yamazaki, T.Akiyama, T.Shindo: "Routine high-performance liquid chromatographic determination of myocaridial interstitial norepinephrine." J Chromato.670. 328-331 (1995)

T.Yamazaki、T.Akiyama、T.Shindo：“心肌间质去甲肾上腺素的常规高效液相色谱测定”。

T.Yamazaki, T.Akiyama: "Cardiac dialysis offers new approach to assessment of cardiac sympathetic nerve endings abnormality" Circualation. 92. I-59 (1995)

T.Yamazaki、T.Akiyama：“心脏透析为评估心脏交感神经末梢异常提供了新方法”。

T. Yamazaki, T. Akiyama, T. Shindo,: "Routine high-performance liquid chromatographic determination of myocaridial interstitial norepinephrine." J Chromato.670. 328-331 (1995)

T. Yamazaki、T. Akiyama、T. Shindo，：“心肌间质去甲肾上腺素的常规高效液相色谱测定”。

北川裕利、秋山剛、山崎登自: "下大静脈閉塞時の心筋間質ノルエピネフリン動態" 心臓. 27. 56-59 (1995)

Yuri Kitakawa、Tsuyoshi Akiyama 和 Toji Yamazaki：“下腔静脉闭塞期间心肌间质去甲肾上腺素动力学” 27. 56-59 (1995)