Basic study on neuronal conrol of heart

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1) The present study describes a high-performance liquid chromatographic-electrochemical detection (HPLC-ECD) system for routine measurement of the low levels of norepinephrine (NE) found in the myocardial interstitial space. In this system, an in vivo detection limit of 100 fg in a 50mul injection was achieved for NE.Using cardiac dialysis technique, 20mu dialysates were sampled from the myocardial interstitial space at 2-min intervals. The basal dialysate NE concentration was 16.6<plus-minus>4.0pg/ml. This low detection limit allowed the dialysate NE concentration to be monitored for dysfunction of the cardiac sympathetic nerve terminal. This system offers a new possibility for routine analysis of myocardial interstitial NE level2) The present study describes a high-performance liquid chromatographic electrochemical detection (HPLC-ECD) system for monitoring of dihydroxyphenylglycol (DHPG) in the myocardial interstitial spsce. Using cardiac dialysis technique, 10mul dialysates were sampled from the myocardial interstitial space (1min-fraction), and directly injected to HPLC-ECD system. An in vivo detection limit of 100 fg in a 10mul injection was achieved for DHPG.The basal dialysate DHPG concentration was 181<plus-minus>46 pg/ml. This system offers a new possibility for monitoring of myocardial interstitial DHPG level