Molecular Characterizaiton of LFA-1-like Molecule on Exocrine Glands in Patients with Malignant Tumor.

恶性肿瘤患者外分泌腺上 LFA-1 样分子的分子特征。

• 批准号: 06670175
• 负责人: YAMADA Akira
• 金额: $ 1.41万
• 依托单位: Kurume University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670175/
• 关键词: LFA-1-like molecule; LFA-1; CD11a; Adhesion molecule; Exocrine glands; Malignant tumors; adenocarcinoma

We previously reported that the immunohistochemically defined LFA-1 antigen (LFA-1-like antigen) was expressed on various exocrine tissues uninvolved by tumors in patients with malignant diseases using LFA-1alpha-specific monoclonal antibodies (mAb) 2F12 and HVS6B6. We investigated in this manuscript differences at the molecular level between LFA-1 on leukocytes and LFA-1-like antigen on MKN-45.16, a subline derived from an adenocarcinoma line MKN45 that expresses a high amount of LFA-1-like antigen. LFA-1-like antigen was reactive to mAb 2F12 or HVS6B6, but not to any of the other five different LFA-1alpha (CD11a) -specific or four LFA-1beta (CD18) -specific mAb. MAb 2F12 immunoprecipitated a 200 KD molecular weight membrane protein (LFA-1-like antigen) from MKN45.16 cells, whereas it precipitated 180 KD (LFA-1alpha) and 95 KD (LFA-1beta) proteins form a monocytic cell line (THP-1) under both non-reducing and reducing conditions. The molecular difference was ocnfirmed further by N-glycanse treatment of the immunoprecipitates. The isoelectric point of LFA-1-like antigen was 6.0, whereas that of LEA-1alpha and LFA-1beta was 6.0 and 4.7, respectively, by two-dimensional gel electrophoresis. Expression of LFA-1alpha gene on MKN45.16 cells was not detected at the mRNA level by six different sets of LFA-1alpha specific oligonucleotides and reverse transcription-polymerase chain reaction. These results indicated that LFA-1-like antigen on an adenocarcinoma cell line was a distinct molecule from LFA-1 on leukocyte.

我们以前报道过，使用LFA-1 α特异性单克隆抗体（mAb）2F 12和HVS 6 B6，化学定义的LFA-1抗原（LFA-1样抗原）在恶性疾病患者的各种外分泌组织中表达。我们在本手稿中研究了白细胞上的LFA-1和MKN-45.16上的LFA-1样抗原之间在分子水平上的差异，MKN-45.16是来自表达大量LFA-1样抗原的腺癌细胞系MKN 45的亚系。LFA-1样抗原与mAb 2F 12或HVS 6 B6反应，但与其他5种不同的LFA-1 α（CD 11 a）特异性或4种LFA-1 β（CD 18）特异性mAb中的任何一种均不反应。在非还原和还原条件下，MAb 2F 12从MKN 45.16细胞免疫沉淀200 KD分子量的膜蛋白（LFA-1样抗原），而从单核细胞系（THP-1）免疫沉淀180 KD（LFA-1 α）和95 KD（LFA-1 β）蛋白。通过N-聚糖处理免疫沉淀物进一步证实了分子差异。双向凝胶电泳结果表明，LFA-1样抗原的等电点为6.0，而莱亚-1 α和LFA-1 β的等电点分别为6.0和4.7。LFA-1 α基因在MKN 45. 16细胞中的表达在mRNA水平上通过6组不同的LFA-1 α特异性寡核苷酸和逆转录聚合酶链反应检测不到。这些结果表明腺癌细胞系上的LFA-1样抗原与白细胞上的LFA-1是不同的分子。

项目成果

T.Hoshino, A.Ohta, M.Nakao, T.Ota, T.Inokuchi, S.Matsueda, R.Gouhara, A.Yamada, K.Itoh, and K.Oizumi: "TcRgammadelta^+ T cells in peripheral blood of patients with adult Still's disease." J.Rheumatol.23. 124-129 (1996)

T.Hoshino、A.Ohta、M.Nakao、T.Ota、T.Inokuchi、S.Matsueda、R.Gouhara、A.Yamada、K.Itoh 和 K.Oizumi：“TcRgammadelta^ 外周血中的 T 细胞

U.Toh, H.Yamana, H.Fujita, Y.Toh, T.Fujii, A.Yamada, S.Shichijo, and K.Itoh: "A monoclonal antibody KIS-1 recognizing a new membrane antigen on human squamous cell carcinoma." Int.J.Cancer. (in press).

U.Toh、H.Yamana、H.Fujita、Y.Toh、T.Fujii、A.Yamada、S.Shichijo 和 K.Itoh：“识别人鳞状细胞癌新膜抗原的单克隆抗体 KIS-1

AKIRA YAMADA: "Immunohistochemically Defined Lymphocyte Function-Associated Antigenl (LFA-1) on an Adenocarcinoma Cell Line Is a Distinct Moleculefrom LFA-1 on Leukocytes" CELLULAR IMMUNOLOGY. 166. 254-260 (1995)

AKIRA YAMADA：“腺癌细胞系上的免疫组织化学确定的淋巴细胞功能相关抗原 (LFA-1) 是与白细胞上的 LFA-1 不同的分子”细胞免疫学。

A.Yamada, A.Kataoka, S.Shichijo, T.Kamura, Y.Imai, T.Nishida, and K.Itoh: "Expression of MAGE-1, MAGE-2, MAGE-3/-6 and MAGE-4a/-4b genes in ovarian tumors." Int.J.Cancer (Pred.Oncol.). 64. 388-393 (1995)

A.Yamada、A.Kataoka、S.Shichijo、T.Kamura、Y.Imai、T.Nishida 和 K.Itoh：“MAGE-1、MAGE-2、MAGE-3/-6 和 MAGE-4a 的表达

Inoue,M: "Close co-localization of CD4 and serin esteras-tryptase TL2 on the cell surface" Bicchem.Bioph Res. Com. 201. 1390-1395 (1994)

Inoue,M：“CD4 和丝氨酸酯类胰蛋白酶 TL2 在细胞表面的紧密共定位”Bicchem.Bioph Res。