In vivo microscopic observation of neutrophil kinetics in pulmonary microcirculation.

肺微循环中性粒细胞动力学的体内显微镜观察。

• 批准号: 06670603
• 负责人: KATOH Kunihiko
• 金额: $ 1.15万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670603/
• 关键词: Pulmonary microcirculation; In vivo microscopy; Ncutrophil; Pulmonary capillary; Pulmonary circulation; White blood ccll; Isolated perfused lobe; 摘出分離潅流肺

The aims of this study were to establish a new method to evaluate neutrophil kiknetics in the pulmonary microcirculation and to clarify the mechanism for neutrophil passage through pulmonary circulation. Neutrophils, that were isolated from normal donor dogs, were labeled with fluorescein dye and injected into normal recipient dogs. And their passage through the pulmonary microcirculation was recorded by in vivo videofluorescence microscopy through a transparent thoracic window. During normal conditions, neutrophils were marginated in neither venules nor arterioles, but sequestered in the pulmonary capillaries. And also these neutrophils stopped exclusively at the junctions of the capillary network. This implies that the relationship between the size of neutrophil and the internal diameter of capillary segment has a significant role in neutrophil sequestration. These results suggests that the mechanism that causes ncutrophils to stop in the pulmonary microcirculation under normal conditions might be simple mechanical impediment.

本研究的目的是建立一种新的方法来评价肺微循环中性粒细胞的变化，并阐明中性粒细胞通过肺循环的机制。从正常供体犬中分离出中性粒细胞，用荧光素染料标记，然后注射到正常受体犬体内。并通过透明的胸窗，通过活体视频荧光显微镜记录它们通过肺微循环的过程。在正常情况下，中性粒细胞在小静脉和小动脉中都没有边缘，但在肺毛细血管中隔离。而且这些中性粒细胞只停留在毛细血管网络的交界处。说明中性粒细胞的大小与毛细血管段内径的关系在中性粒细胞的隔离中起着重要作用。这些结果表明，在正常情况下，中性粒细胞在肺微循环中停止的机制可能是简单的机械障碍。

项目成果

Osamu Okada: "Stability of alveolar capillary opening pressure." J.Apple.Physiol.77. 1630-1637 (1994)

Osamu Okada：“肺泡毛细血管开放压力的稳定性。”

Okada O., Presson R.G., Jr., Godbey P.S., Capen R.L., and Wagner W.W., Jr.: "Temporal capillary perfusion patterns in single alveolar walls of intact dogs." J Appl Physiol. 76. 380-386 (1994)

Okada O.、Presson R.G., Jr.、Godbey P.S.、Capen R.L. 和 Wagner W.W., Jr.：“完整狗的单个肺泡壁中的颞毛细血管灌注模式。”

Osamu Okada: "Temporal capillary perfusion patterns in single alveolar walls of intact dogs" J.Apple.Physiol.76. 380-386 (1994)

Osamu Okada：“完整狗的单个肺泡壁中的颞毛细血管灌注模式”J.Apple.Physiol.76。

Osamu Okada: "Stability of alveolar capillary opening pressure" J. Appl. Physio. 77. 1630-1637 (1994)

Osamu Okada：“肺泡毛细血管开放压力的稳定性”J. Appl。

Presson R.G., Jr., Okada O., Hanger C.C., Godbey P.S., Greham J.A., Glnny R.W., Capen R.L., and Wagner W.W., Jr.: "Stability of alveolar capillary opening pressures." J Appl Physiol. 77. 1630-1637 (1994)

Presson R.G., Jr.、Okada O.、Hanger C.C.、Godbey P.S.、Greham J.A.、Glnny R.W.、Capen R.L. 和 Wagner W.W., Jr.：“肺泡毛细血管开放压力的稳定性。”