INVENTION DISCLOSURES - CENTER FOR IN VIVO MICROSCOPY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在本报告所述期间，向杜克大学科学和技术办公室提交了两项发明披露： 2007年12月：Cristian T.Badea，Samuel Johnston，G.Allan Johnson：在单一断层重建中结合使用多个成像链获取的投影图像的几何校准程序 本文提出了第一种适用于双或多象素微型CT系统的几何定标方法。同样的技术可以用于两个以上的成像链。我们不知道关于这一主题的其他已发表的先前工作。由于我们的方法涉及到每条链的几何校准，所以我们只能比较 5.虽然我们部分使用了与Yang等人相同的公式来初步估计五个几何参数，但对于单链，我们的方法由于在优化步骤的基础上增加了精化而显示出优势。基于优化的细化提高了重建的质量。我们的结果表明，我们还找到了包括z和a在内的双系统参数的准确和精确值。 2007年12月：G.Allan Johnson，Ph.D.，Gabriel Howles-Banerji，Kristin Frinkley，Kathy Nightingale，Ph.D.，Mark Palmeri，M.D.，Ph.D.：开发、实施并测试了打开血脑屏障和活性染色的方法。提供了对超声方法的机理的见解。 我们描述了一种主动染色来增强活体动物大脑中的MR信号的方法，即活体对比增强脑成像。主动染色方法可用于显著提高空间分辨率，比以前所见的分辨率大5倍以上。同样的方法可以用于特定的探针和实验方案，以提供活体啮齿动物神经元活动的定量定位测量：小鼠或大鼠大脑的功能性磁共振成像，同样是在空间分辨率大大高于先前展示的情况下。最后，通过合适的探针和方案，该方法有望结合非常高分辨率的活体3D解剖学定义来帮助确定大脑中特定分子目标的密度。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This reporting period, 2 invention disclosures were submitted to the Duke Office of Science and Technology: 12/2007: Cristian T. Badea, Samuel Johnston, G. Allan Johnson: Geometric Calibration Procedure for the Combination of Projection Images Acquired with Multiple Imaging Chains in a Single Tomographic Reconstruction We have presented here the first geometric calibration method suitable for a dual or multipel micro-CT system. The same technique can be used for more than two imaging chains. We are not aware of other published prior work on this subject. Since our method involves the geometric calibration of each individual chain, we could only compare part of our results with the previous work 5. Although we partially use the same formulas as Yang et al.5to initially estimate five of the geometric parameters, our method for single chain show advantages due to added refinement based on optimization step. The optimization based refinements improve the quality of reconstruction. Our results demonstrate that we also find accurate and precise values for the dual system parameters including the ¿z and a . 12/2007: G. Allan Johnson, Ph.D., Gabriel Howles-Banerji, Kristin Frinkley, Kathy Nightingale, Ph.D., Mark Palmeri, M.D., Ph.D.: Active staining for in vivo MR imaging in the brain Developed, implemented, and tested the methods for opening the blood-brain barrier and active staining. Provided insights into ultrasonic mechanisms of method. We describe a method for active staining to enhance the MR signal in the brain of live animals, i.e., in vivo contrast-enhanced brain imaging. The active staining method can be used to provide substantial increase in spatial resolutionmore than 5X greater than what has been seen previously. The same method can be used with specific probes and experimental protocols to provide quantitative localized measure of neuronal activity in the live rodent: functional MR imaging of the mouse or rat brain, again at spatial resolution substantially greater than has been demonstrated previously. Finally, with suitable probes and protocols, the method promises to help define the density of specific molecular targets in the brain in combination with very high-resolution 3D in vivo anatomic definition.