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Apolipoprotein Phenotypes in Healthy Controls and Demented Patients from the Inhabitants in the middle and Northern mie Prefecture of Japan

日本三重县中北部居民健康对照和痴呆患者的载脂蛋白表型

基本信息

批准号：
06670650
负责人：
KUZUHARA Shigeki
金额：
$ 1.22万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

To clarify the association of apolipoprotein E4 (apo E4) with the pathognenesis of Alzehimer's disease (AD) , we analyzed the distribution of apolipoprotein E (apo E) phenotype and the ealculated the frequency of epsilon2, epsilon4 of 692 healthy controls and 75 demented patients from the inhabitants in the middle and nothern areas of Mie Prefecture of Japan.Demented subjects included 13 patients with early onset Alzheimer's desease group (EOAD) , 30 patients with late onset Alzheimer's disease group (LOAD) , and 30 patients with vascular dementia group (VD). The apolipoprotein E phenotype was determined by the isoelectric focusing and immunoblotting. The_X^2 test was used in statical analysis.There were no significant differences between sexes or among ages in the frequency of each apo E phenotype, and the estimated frequency of epsilon2, epsilon3, and epsilon4 was 0.05,0.85 and 0.10 respectively in normal and elderly controls. There was a significant difference in the frequency of apo E phenotypes between the patients with LOAD and elderly controls aged 60 years or older (p<0.0001) , but there was no statiscal difference between the elderly controls and EOAD or VD group. The frequency of epsilon3 was 0.77 in EOAD,0.60 in LOAD and 0.81 in VD,and the frequency of epsilon4 was 0.19 in EOAD,0.38 in LOAD and 0.13 in VD.In the patients with LOAD,epsilon3 frequency was significantly lower (p<0.0001) and epsilon4 frequency was significantly higher (p<0.0001) than that in the controls.The frequency of apo E4 was significantly high in patients with LOAD,and the apo E4 is a possible candidate of one of the risk factors of development of LOAD.

为探讨载脂蛋白E4（apoE4）与阿尔茨海默病（Alzehimer 'sdisease，AD）发病的关系，分析了AD患者的载脂蛋白E（apoE）表型分布，并计算了ε 2的频率。对日本三重县中北部地区692名健康人和75名痴呆患者进行了E_4测定，痴呆患者包括13名早期老年痴呆组（EOAD）、晚发性阿尔茨海默病组（LOAD）30例、血管性痴呆组（VD）30例。采用等电聚焦和免疫印迹法测定载脂蛋白E表型。统计学分析采用X^2检验，apoE各表型的频率在性别和年龄间均无显著性差异，ε 2、ε 3和ε 4在正常人和老年人中的估计频率分别为0.05、0.85和0.10。LOAD组与60岁以上老年对照组的apo E表型频率有显著性差异（p<0.0001），而与EOAD组和VD组的apo E表型频率无显著性差异。ε 3在EOAD、LOAD和VD中的频率分别为0.77、0.60和0.81，ε 4在EOAD、LOAD和VD中的频率分别为0.19、0.38和0.13。ε 3频率显著降低（p<0.0001）和ε 4频率显著高于（p<0.0001），LOAD患者载脂蛋白E4的频率显著增高，载脂蛋白E4可能是LOAD发生的危险因素之一。