Myocardial damage by cultured autologous lymphocytes with IL-2 in viral myocarditis

IL-2培养自体淋巴细胞对病毒性心肌炎的心肌损伤

• 批准号: 06670702
• 负责人: KISHIMOTO Chiharu
• 金额: $ 1.28万
• 依托单位: TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670702/
• 关键词: Viral myocarditis; Dilated cardiomyopathy; Myocardial damage; Interleukin-2; 拡張型心筋症

Based on the clinical and experimental data, infiltrating T lymphocyte into the myocardium is a key element of the pathogenesis of myocarditis. The aim of this study is to clarify the factors for variation of the severity of myocarditis. We carried out the lymphocyte culture from murine coxsackievirus B3 myocarditis specimens with interleukin-2, with the anakysis of myocardial lymphocyte subsets. Cultured lymphocytes were transfered into inbred strains of mice. The predominance of CD4^+ cells in cultured lymphocytes correlated not only with the in situ distribution of myocardial lymphocyte subsets but with the severity of myocarditis among murine strains. Myocardial CD4^+ lymphocytes may be related to the severity of myocarditis and pathogenesis of the transition into chroniic myocardial disease.

临床和实验研究表明，T淋巴细胞浸润心肌是心肌炎发病的关键因素。本研究的目的是阐明心肌炎严重程度的变化因素。用白细胞介素-2对小鼠柯萨奇B3病毒性心肌炎标本进行淋巴细胞培养，并对心肌淋巴细胞亚群进行分析。将培养的淋巴细胞转移到近交系小鼠中。培养的淋巴细胞中CD 4 ^+细胞的优势不仅与心肌淋巴细胞亚群的原位分布有关，而且与小鼠品系心肌炎的严重程度有关。心肌CD 4 ^+淋巴细胞可能与心肌炎的严重程度和向慢性心肌病转变的发病机制有关。

项目成果

Chiharu Kishimoto et al.: "Therapy with captopril suppresses fibrin dipositions and induces ventricular remodeling in coxsackievirus B3 myocarditis." American Journal of Physiology. (in press).

Chiharu Kishimoto 等人：“卡托普利治疗可抑制柯萨奇病毒 B3 型心肌炎中的纤维蛋白沉积并诱导心室重塑。”

Kishimoto C,Kurokawa M,Ochiai H,Takada H,Hiraoka Y,Sasayama S,Shiraki K: "Analysis of the pathogenesis of coxsackievirus B3 myocarditis. Comparison of myocarditic and amyocarditic coxsackievirus B3 strains." Nagano M,Takeda N,Dhalla NS,eds. The cardiomyop

Kishimoto C，Kurokawa M，Ochiai H，Takada H，Hiraoka Y，Sasayama S，Shiraki K：“柯萨奇病毒 B3 型心肌炎的发病机制分析。心肌炎和心肌炎型柯萨奇病毒 B3 株的比较。”

Kishimoto C,Kuroki Y,Hiraoka Y,Ochiai H,Sasayama S: "Lymphokine-activated killer cells in myocarditis." Basic Res. in Cardiol. (in press).

Kishimoto C、Kuroki Y、Hiraoka Y、Ochiai H、Sasayama S：“心肌炎中淋巴因子激活的杀伤细胞。”

Kishimoto C,Hiraoka Y,Takada H,Suzuki N,Shiraki K: "Effects of granulocyte colony-stimulating factor upon coxsackievirus B3 myocarditis in mice." Eur. Heart J.16. 25-33 (1995)

Kishimoto C、Hiraoka Y、Takada H、Suzuki N、Shiraki K：“粒细胞集落刺激因子对小鼠柯萨奇病毒 B3 心肌炎的影响。”

Kishimoto C et al.: "Cytokine and murine coxsackievirus B3 myocarditis" Circulation. 89. 2836-2842 (1994)

Kishimoto C 等人：“细胞因子和鼠科萨奇病毒 B3 心肌炎”循环。