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Analyses of myocardial epitopes and apoptosis with treatment of peptide therapy in myocarditis.

心肌炎肽疗法治疗心肌表位和细胞凋亡的分析。

基本信息

批准号：
09470164
负责人：
KISHIMOTO Chiharu
金额：
$ 5.57万
依托单位：
KYOTO UNIVERSITY (1998-2000)Toyama Medical and Pharmaceutical University (1997)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-09470164/
关键词：
Viral myocarditis Dilated cardiomyopathy Coxsackievirus B3 Apoptosis Epitopes Peptide therapy

项目摘要

To clarify the pathogenesis and the development of coxsackievirus B3 (CVB3) myocarditis, we examined the histopathology of CVB3-inoculated wild type and severe combined immunodeficient C3H/He mice, and analyzed using subcloning method the sequence of nucleotides of an amyocarditic strain of CVB3 (CVBO) with comparison of that of a myocarditic strain of CVB3 (CVBM), which was already reported. Severe myocarditis developed in wild mice inoculated with CVBM.On the contrary, mild myocarditis resulted from wild mice inoculated with CVBO.In scid mice, severe myocarditis developed by the inoculation of both CVBM and CVBO.There were seven nucleotide differences with five corresponding amino acid changes in the sequence of CVBO in comparison with that of CVBM.The changes located in 1A, 1C, and 1D regions of viral genome, which encoded the envelope protein. Thus, antigenic determinant of CVB3 is related, at least in part, to the recognition of immunocompetent cells to the difference of nucleotides in these regions. In conclusion, the development of myocarditis is related to the immunological background of the host, and myocarditis is due, in part, to an immunopathogenic phenomenon.

为阐明柯萨奇病毒B3（CVB 3）心肌炎的发病机制和发展过程，我们观察了CVB 3感染野生型和严重联合免疫缺陷C3 H/He小鼠的组织病理学变化，并采用亚克隆技术对CVB 3心肌炎株（CVBO）和已报道的CVB 3心肌炎株（CVBM）的核苷酸序列进行了比较分析。结果表明，CVBO与CVBM相比，在1A、1C和1D区有7个核苷酸的差异，其中5个氨基酸发生了相应的变化，而在1B区有5个氨基酸的变化，其中1A区的变化最明显，1C区的变化最明显，1D区的变化最明显，1B区的变化最明显，1C区的变化最明显，1D区的变化最明显，1A区的变化最明显，1C区的变化最明显，1D区的变化最明显，1C区的变化最明显，1D区的变化最明显。它编码包膜蛋白。因此，CVB 3的抗原决定簇至少部分地与免疫活性细胞对这些区域中核苷酸差异的识别有关。总之，心肌炎的发展与宿主的免疫背景有关，心肌炎部分是由于免疫病理现象。