Molecular biological changes of ADP/ATP carrier in cardiomypathic hamster

ADP/ATP载体蛋白(AAC)是存在于线粒体内膜上的一种完整的蛋白质，负责ADP和ATP的胞浆和线粒体内交换。J-2-N仓鼠的AAC含量显著降低。通过分子生物学分析，采用空斑杂交法克隆了金黄地鼠AAC基因。AAC基因与AAC基因特异性杂交，进行RNA斑点杂交。AAC基因表达水平在对照组仓鼠中最高，其次是轻度心肌损伤的J-2-N仓鼠、严重心肌损伤的J-2-N仓鼠和Bio 14.6心肌病仓鼠。这些结果提示AAC含量降低可能参与了心肌病的发病机制，AAC基因表达水平的降低可能是J-2-N心肌病仓鼠AAC异常的原因。

ADP/ATP carrier protein (AAC) is an integral protein present in the inner mitochondrial membrane that performs the exchange of cytoplasmic and intramitochondrial ADP and ATP.AAC content of myocardium was studied in J-2-N cardiomyopathic hamsters. The AAC content was significantly decreased in the J-2-N hamsters. By molecular biological analysis, hamster AAC cDNA was cloned by the plaque hybridization method. The AAC cDNA hybridized specifically with AAC mRNA,so RNA dot-blot hybridization was performed. The highest AAC mRNA level was observed in control hamsters followed by J-2-N hamsters with mild myocardial damage, J-2-N hamsters with severe myocardial damage and Bio 14.6 cardiomyopathic hamsters. These results suggest that a decreased AAC content may contribute to the pathogenesis of cardiomyopathy and that a decrease of AAC mRNA levels may explain the abnormalities of AAC in J-2-N cardiomyopathic hamsters.