Growth Hormone Secretion and Behavioral States in Neonate.

本研究旨在探讨婴幼儿行为状态与生长激素(GH)分泌的关系。通过连续录像观察动物的行为状态，同时每隔20~30分钟采血一次。但在录像结束时，我们只从每个婴儿身上采集了一份血液样本，因为我们不可能经常从生病的婴儿身上提取血液样本。用高灵敏的双抗体夹心酶免疫分析法(EIA)测定血浆GH浓度。从本研究的数据来看，我们不能确定行为状态与GH分泌之间的关系。在我们的研究中，一些婴儿，特别是早产儿，表现出如此频繁的状态变化，以至于我们不能判断他们是真正的状态周期。以前有一些关于状态与GH分泌关系的研究，但每一项研究的结论都是不一致的。有人认为，对行为状态的误判是造成这种不一致的原因。因此，我们认为应重新考虑观察状态的持续时间和GH检测的采血时间。通过长期的数据积累，孕后年龄、围产期窘迫和中枢神经系统紊乱等因素的影响将变得清晰。

This study was aimed to make clear the relation between the behavioral states and growth hormone (GH) secretion in infants. We designed to observe the behavioral states by continuous video recording and at the same time take small amounts of blood every 20 to 30 minutes. But we took only one blood sample at the end of the video recording from each infant because it was impossible for us to take blood samples frequently from the sick infants. Plasma GH concentrations were measured by the highly sensitive sandwich enzyme immunoassay (EIA). From the data of this study, we couldn't confirm the relation between the behavioral states and GH secretion. In our study some infants, especially premature ones, showed the changes of states so frequently that we couldn't judge them as the true state cycle. Previously some study about the relation between states and GH secretion were reported, but the conclusions of each study are inconsistent. It was suggested that the misjudgments of the behavioral states were the reason of that inconsistency. So it is thought that we should reconsider the duration to observe the states and the timing of taking blood samples for GH assay. By accumurating data over a long period, the effects of factors such as postconceptional age, perinatal distress and the disorders of central nervous system will be clear.