Regulation of CRF gene expression in the hypothalamus

下丘脑CRF基因表达的调控

• 批准号: 06670998
• 负责人: ITOI Keiichi
• 金额: $ 1.34万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670998/
• 关键词: hypothalamus; CRF; ACTH; neural pathway; gene; mRNA; rat; pituitary

Acetylcholine (ACh) is one of the major neurotransmitters in the brain. ACh-containing nerve terminals are present in the vicinity of corticotropin-releasing factor (CRF) neurons in the paraventricular nucleus of the hypothalamus (PVN).To investigate the role of ACh on CRF synthesis and release ACh was injected directly into the PVN of conscious rats, and changes in CRF mRNA levels in the hypothalamus, POMC mRNA levels in the anterior pituitary (AP) as well as plasma ACTH levels were monitored.1. Plasma ACTH increased following ACh (1-100 pmol) injection into the PVN of conscious rats in a dose-dependent manner, reaching the peak at 30 min and returning to the baseline at 120 min.2. POMC mRNA in the AP and CRF mRNA in the hypothalamic tissue increased dose-dependently 120 min after injection of ACh (0.1-10pmol) into the PVN.3. Pretreatment with icv atropine abolished the ACh-induced increase in plasma ACTH whereas pretreatment with icv hexamethonium had no effect.These results suggest that ACh stimulates CRF synthesis and secretion. Thus, cholinergic inputs to the hypothalamus may regulate positively the CRF neurons in the PVN.The cholinergic influence may be mediated by muscarinic receptors.

乙酰胆碱（Acetylcholine，ACh）是脑内主要的神经递质之一。乙酰胆碱（ACh）神经末梢位于下丘脑室旁核（PVN）促肾上腺皮质激素释放因子（CRF）神经元附近，为探讨ACh对CRF合成和释放的影响，将ACh直接注入清醒大鼠PVN，观察下丘脑CRF mRNA、垂体前叶POMC mRNA及血浆ACTH水平的变化.清醒大鼠PVN注射ACh（1-100 pmol）后，血浆ACTH呈剂量依赖性升高，30 min达高峰，120 min恢复至基线水平. PVN注射ACh（0.1- 10 pmol）后120 min，AP中POMC mRNA和下丘脑组织中CRF mRNA均呈剂量依赖性增加. icv阿托品预处理可抑制ACh引起的血浆ACTH升高，而icv六烃季胺预处理则无此作用，提示ACh可刺激CRF的合成和分泌。因此，下丘脑的胆碱能输入可正向调节室旁核的CRF神经元，这种胆碱能影响可能是由M受体介导的。

项目成果

井樋慶一: "脳内ノルエピネフリン作動性神経路による視床下部-下垂体-副腎系の調節機構" ACTH Reloted Peptides. 5. 27-34 (1994)

Keiichi Ibi：“大脑中去甲肾上腺素能通路对下丘脑-垂体-肾上腺轴的调节机制”ACTH Reloted Peptides。 5. 27-34 (1994)

Keiichi Itoi: "Microinjection of norepinephrine into the paraventricular nucles of the hypothalomus stimulates cotticotropin-releasing factor gene expression in conscious rats" Endocrinology. 135. 2177-2182 (1994)

Keiichi Itoi：“将去甲肾上腺素显微注射到下丘脑室旁核中可刺激清醒大鼠促促肾上腺皮质激素释放因子基因表达”内分泌学。

井樋慶一: "脳内ノルエピネフリン作動性神経路による視床下部-下垂体-副腎系の調節機構" ACTH Related Peptides. 5. 27-34 (1994)

Keiichi Ihi：“大脑中去甲肾上腺素能通路对下丘脑-垂体-肾上腺轴的调节机制”ACTH 相关肽。5. 27-34 (1994)

Itoi K et al: "Microinjection of norepinephrine into the paraventricular nucleus of the hypothalamus stimulates corticotropin-conscious rats" Endocrinology. 135. 2177-82 (1994)

Itoi K 等人：“将去甲肾上腺素显微注射到下丘脑室旁核会刺激促肾上腺皮质激素意识的大鼠”内分泌学。

Ohmori N et al: "Effect of acetylcholine on corticotropin-releasing factor gene expression in the hypothalamic paraventricular nucleus of conscious rats" Endocrinology. 136. 4858-63 (1995)

Ohmori N 等人：“乙酰胆碱对清醒大鼠下丘脑室旁核促肾上腺皮质激素释放因子基因表达的影响”内分泌学。