透析患者にみられる多嚢胞化萎縮腎と腎細胞癌に関する研究

透析患者多囊萎缩肾和肾细胞癌的研究

• 批准号: 06671161
• 负责人: 石川 勲
• 金额: $ 1.22万
• 依托单位: Kanazawa Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671161/
• 关键词: 血液透析; 腎嚢胞; 腎細胞癌; 核型

透析患者には悪性腫瘍の中でも腎細胞癌の発生が著しく高い。これは病腎に嚢胞化が起こるためと考えられている。本研究では透析患者の腎細胞癌が嚢胞-非定型嚢胞-腺腫-腎細胞癌と連続性を持って移行することをトリソミ-の観察から見出そうとした。まず透析患者20症例の多嚢胞化萎縮腎に合併した腎細胞癌を培養しそのKaryotypeと末梢血のKaryotypeを比較検討した。壊死組織などで腫瘍細胞が培養できなかったものをのぞき、12例中現在までに結果の出ている10例について報告する。10例中7例は乳頭型、3例は非乳頭型腎細胞癌であった。乳頭型の染色体数をみると染色体2,3,4番に増加(トリソミ-)1例ずつ、5番に増加2例と減少(モノソミ-)1例、6番に減少1例、7番に増加1例、8番に増加1例と減少1例、12番に増加2例、13番に増加1例と減少1例、16番い増加4例、17番に増加1例と減少1例、18番に減少2例、20番に増加2例、22番に増加1例と減少1例、Xに増加1例と減少1例、Yに減少3例がえられ、それ故乳頭型では7例すべてに染色体の数の異常ととくに16番の増加(トリソミ-)とY染色体の欠失が目立つという新しい所見がえられた。また非乳頭型では3例中2例にKaryotypeの異常は見出せず、残る1例に染色体の数の異常が見出された。次にこれら染色体の数の異常が嚢胞、非定型嚢胞、腺腫のどの段階から起こっているかを知るため病変部と対比しつつ染色体数の異常をパラフィン切片から検出しようと試みた。検出には各染色体のDNAプローブを用いIn situ hybridizationによる細胞内シグナルを染色、顕微鏡下で核内の数の異常を用いた。実際に行ってみると、パラフィン切片作成条件に伴う差、プロティナーゼKの濃度・処理時間による差、プローブによる差など種々の解決すべき問題が出現し、現在のところ、ある条件下でホルマリン固定した腎細胞癌でしか成功していない。

The incidence of renal cell carcinoma in dialysis patients is very high. The disease is caused by kidney degeneration. This study was conducted to investigate the relationship between renal cell carcinoma (RCC), atypical RCC, and renal cell carcinoma (RCC). A comparative study of Karyotype and Karyotype in peripheral blood of 20 patients with multicellular atrophic kidney and renal cell carcinoma In 12 cases, the tumor cells were cultured and the results were reported in 10 cases. Among 10 cases, 7 cases were papillary type and 3 cases were nonpapillary type. 1 case of papillary chromosome number increase, 2 cases of chromosome number increase, 1 case of chromosome number decrease, 2 cases of chromosome number increase, 1 case of chromosome number decrease, 1 case of chromosome number increase, 1 case of chromosome number increase, 1 case of chromosome number decrease, 2 cases of chromosome number increase, 1 case of chromosome number increase, 1 case of chromosome number decrease, 2 cases of chromosome number increase, 1 case of chromosome number increase, 1 case of chromosome number decrease, 2 cases of chromosome number increase, 1 case of chromosome number increase, 1 case of chromosome number decrease, 2 cases of chromosome number increase, 2 cases of chromosome number increase, 1 case of chromosome number increase, 1 case of chromosome number decrease, 2 cases of chromosome number increase, 1 case of chromosome number increase, 2 cases of chromosome number increase, 1 case of chromosome number increase, 1 case of chromosome number increase, 2 cases of chromosome number increase, 1 case of chromosome number increase, 2 cases of chromosome number increase, 1 case of chromosome number increase, 1 case of chromosome number increase, 2 cases of chromosome number increase, 1 case of chromosome number increase, 2 cases of chromosome number increase, 1 case of chromosome number increase, 1 case of 22 chromosome increase 1 case, X chromosome increase 1 case, Y chromosome decrease 3 cases, so nipple type 7 cases, chromosome number abnormality 16 cases, Y chromosome increase 16 cases. Karyotype abnormalities were found in 2 of 3 cases of non-papillary type, and chromosome number abnormalities were found in 1 case of residual type. The number of abnormal chromosomes in the next few years, such as abnormal cells, atypical cells, adenomas, etc., has been detected in the next few years. In situ hybridization, intracellular DNA staining, and microscopical analysis of chromosomal DNA abnormalities. In the process of preparation of slices, there are differences in the concentration of K, the treatment time, the concentration of K, and the solution of the problem.