Hepatic denervation : Hemodynamic changes

肝去神经支配：血流动力学变化

• 批准号: 06671224
• 负责人: SATO Koshi
• 金额: $ 1.34万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671224/
• 关键词: Hepatic denervation; Hemodynamic change; Dopamine; Liver Transplantation; 門脈血流量; 肝動脈血流量; 心拍出量; 肝神経切断; 肝移植; 肝血流量; 酸素飽和度

[Objective]Hepatic nerves are often removed during liver transplantation and hepato biliary surgery. At the present time, the hemodynamic condition after hepatic nerve denervation, is not clear. We assessed the hepatic hemodynamic condition and the effect of dopamine after hepatic denervation.[Material and methods]For this experiment, we used mongrel dogs. The dogs were anesthesized with an IV administretion of pentobarbital, then intubated with an intratrachial tube and maintained by mechanical ventilation. A midline incision was performed to open the abdomen. All visible nerves in the hepatoduodnal ligament and around the liver were removed. The mean arterial pressure (MAP), central venous pressure (CVP), pulmonary arterial pressure (PAP), pulmonary arterial wedge pressure (PAWP), cardiac output (CO), portal vein pressure (PVP), hepatic arterial flow (HAF) and hepatic tissue flow (HTF) were monitered. The dogs were divided into three groups. Group 1 : Control group 3 g/kg/min dopamine was administered without liver denervation. Group 2 : 3 g/kg/min dopamine was given 1 hour after liver denervation. Group 3 : 3 g/kg/min dopamine was given 1 week after liver denervation.[Reults]1) PVF/CO increased significantly after the administration of 3 g/kg/min dopamine in group 2 however, it did not change in group 1 and group 3.2) HAF/CO did not change after the administration of 3 g/kg/min dopamine in group 1 and 3 however, it decreased significantly in group 2.3) Total hepatic flow increased significantly after the administration of 3 g/kg/min dopamine in group 1 however, it did not change in group 2 and group 3.[Conclusion]The effects of dopamine administration are similar in group 1 and group 3. These results suggest that the denerveted hepatic nerves were regenerated 1 week after hepatic nerve denervation.

[目的]在肝移植和肝胆外科手术中，经常需要切除肝神经。目前，肝神经失神经支配后的血流动力学状况尚不清楚。我们评估了肝脏去神经后的血流动力学状况和多巴胺的影响。[材料与方法]本实验采用杂种犬。用戊巴比妥静脉注射麻醉犬，然后用气管内插管插管并通过机械通气维持。进行中线切口以打开腹部。切除肝十二指肠韧带和肝脏周围的所有可见神经。监测平均动脉压（MAP）、中心静脉压（CVP）、肺动脉压（PAP）、肺动脉楔压（PAWP）、心输出量（CO）、门静脉压（PVP）、肝动脉血流量（HAF）和肝组织血流量（HTF）。这些狗被分成三组。第1组：对照组，给予多巴胺3 g/kg/min，不进行肝脏去神经支配。第2组：去肝神经后1h给予多巴胺3g/kg/min。第3组：去肝神经后1周给予多巴胺3g/kg/min。[结果]1）3 g/kg/min多巴胺组PVF/CO明显升高，而1、3组无明显变化; 2）3 g/kg/min多巴胺组HAF/CO无明显变化，而1、3组HAF/CO无明显变化。3）在组1中施用3g/kg/min多巴胺后，总肝流量显著增加，第2组和第3组无变化。[结论]第1组和第3组的多巴胺给药效果相似。结果提示，去神经后1周，肝神经即有再生。

项目成果

柿田章: "肝臓の神経の変性と再生に関する電子顕微鏡的研究" 解剖学雑誌. 64. 143-153 (1994)

Akira Kakita：“肝神经变性和再生的电子显微镜研究”解剖学杂志 64. 143-153 (1994)。

柿田章: "肝臓の神経の変性と再生に関する電子顕微鏡的研究" 解剖学雑誌. 69. 143-153 (1994)

Akira Kakita：“肝神经变性和再生的电子显微镜研究”解剖学杂志 69. 143-153 (1994)。