Protective effect of ADF in reperfusion injury after canine by transplantation

人硫氧还蛋白是一种成人T细胞白血病衍生因子，具有多种生理活性，其中一种是自由基清除作用。在犬肺移植模型中评估其减少再灌注损伤的能力。19只犬在热缺血100分钟后进行左肺移植。夹闭对侧肺动脉后评价移植肺的功能。人硫氧还蛋白组（n=6）在再灌注期间给予人硫氧还蛋白30 mg/kg。在N-乙酰半胱氨酸组（n=5）中，以相同的方式给予已知为自由基清除剂的N-乙酰半胱氨酸150 mg/kg。两组患者的动脉血氧分压均显著高于对照组（n=8）。在人硫氧还蛋白组中，吸气峰压显著低于对照组。肉眼和显微镜检查显示，人硫氧还蛋白和N-乙酰半胱氨酸组的肺组织外观几乎正常，与对照组的异常结果形成对比。因此，似乎人硫氧还蛋白对移植的肺具有保护作用，N-乙酰半胱氨酸也是如此，并且其作用可能是自由基清除剂作用。

Human thioredoxin, which was previously recognized as adult T-cell leukemia-derived factor, has many physiologic activities, one of which is a radical scavenger effect. Its ability to reduce reperfusion injury was assessed in vivo in a canine lung transplantation model. In 19 dogs, left lung allotransplantation was performed after 100 minutes of warm ischemia. The function of the transplanted lung was assessed after clamping of the contralateral pulmonary artery. In the human thioredoxin group (n=6), human thioredoxin 30 mg/kg was given to the recipients during reperfusion. In the N-acetylcysteine group (n=5), N-acetylcysteine 150 mg/kg, known as a radical scavenger, was given in the same manner. In both groups, arterial oxygen tension was significantly higher than in the control group (n=8). In the human thioredoxin group, peak inspiratory pressure was significantly lower than in the control group. Macroscopic and microscopic examinations showed an almost normal appearance of the lung tissues in the human thioredoxin and N-acetylcysteine groups, in contrast to the abnormal findings in the control group. Thus it would appear that human thioredoxin has a protective effect on transplanted lungs, as does N-acetylcysteine, and that its action may be a radical scavenger effect.