The experimental study of the induction of tissue regeneration filling in the thoracic cavity using the bioabsorbable materials slowly releasing growth factors.

利用生物可吸收材料缓释生长因子诱导胸腔组织再生充盈的实验研究。

• 批准号: 15390418
• 负责人: YOKOMISE Hiroyasu
• 金额: $ 6.34万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390418/
• 关键词: gelatin micro-spheres; lactic acid and caprolactone copolymer cube; granulation; thoracic cavity; basic fibroblast growth factor; rabbits; pneumonectomy; inflammatory cells; 全摘後症候群; 治療; ポリ乳酸; カプロラクトン; 組織再生; FGF徐放化

We investigated whether the lactic acid and caprolactone copolymer cube (PLA-C) induce granulation in the thoracic cavity and evaluated the efficiency of sprinkled basic fibroblast growth factor slow releasing gelatin micro-spheres (FGF-GMS).Six rabbits underwent pneumonectomy as the control. The PLA-Cs were placed in the post pneumonectomy cavity in the other 4 as the PLA-C group. The PLA-Cs were placed in the cavity with sprinkling FGF-GMS in the last 6 as the PLA-C+FGF group. We examined the excised materials in each group at 1, 2 and 3 months, respectively.In the control, there was no growth of granulation. The fibrous collagen layer covered on the every implanted PLA-Cs. Induction of granulation was limited inside of the PLA-C, started from peripheral area of the PLA-C and continued at 3 months in the PLA-C group but decayed in the PLA-C+FGF groups. The inflammatory cells invaded into the PLA-C along the septum of the PLA-C. FGF-GMS conducted the fibroblasts invasion into the pores and accelerated the thickening of the covering fibrous layer.The granulation could be controlled by the size of the PLA-C. The degradation periods of PLA-C might be controlled by the size of the PLA-C, its pore size and additional FGF-GMS.

观察乳酸-己内酯共聚物块（PLA-C）对家兔胸腔肉芽形成的影响，以及碱性成纤维细胞生长因子缓释明胶微球（FGF-GMS）对胸腔肉芽形成的影响，并以6只家兔肺切除术作为对照。PLA-C组4只，将PLA-C植入肺切除术后的肺腔中。PLA-C+FGF组：PLA-C+FGF组;分别于术后1、2、3个月对各组切取的材料进行检查，对照组未见肉芽生长。植入的PLA-Cs表面均覆盖有胶原纤维层。在PLA-C组中，肉芽的诱导局限于PLA-C内部，从PLA-C的外周区域开始，并在3个月时持续，但在PLA-C+FGF组中减弱。炎性细胞沿着PLA-C的间隔沿着侵入PLA-C。FGF-GMS引导成纤维细胞侵入孔隙，促进覆盖纤维层增厚，颗粒化程度受PLA-C大小控制。PLA-C的降解周期可能受PLA-C的尺寸、孔径和添加FGF-GMS的控制。

项目成果

Bioabsorbable artificial trachea with slow release of bone morphogenetic protein (BMP)-2 : a comparative evaluation of two different suturing techniques.

缓慢释放骨形态发生蛋白（BMP）-2的生物可吸收人工气管：两种不同缝合技术的比较评估。

• 发表时间: 2005
• 期刊: ASAIO Journal (submitted)
• 作者: Yasumichi Yamamoto;Sung Soo Chang;Hitoshi Igai;Noriyuki Misaki;Masashi Gotoh;Hiroyasu Yokomise;Taku Okamoto;Masaya Yamamoto;Yasuhiko Tabata
• 通讯作者: Yasuhiko Tabata

Cartilage regeneration using slow release of bone morphogenetic protein (BMP)-2 from a gelatin sponge to treat experimental canine tracheomalacia : a preliminary report.

使用明胶海绵缓慢释放骨形态发生蛋白 (BMP)-2 来治疗实验性犬气管软化症的软骨再生：初步报告。

• 发表时间: 2003
• 期刊: ASAIO J. 49
• 作者: Taku Okamoto;Yasumichi Yamamoto;Masashi Gotoh;Dage Liu;Minoru Kihara;Kotaro Kameyama;Eiichi Hayashi;Kenji Nakamura;Akira Yamauchi;Cheng-long Huang;Hiroyasu Yokomise;Masaya Yamamoto;Tatsuo Nakamura;Yasuhiko Shimizu;Yasuhiko Tabata
• 通讯作者: Yasuhiko Tabata

Induction of granulation inside of the lactic acid and caprolactone copolymer cube in rabbit's post pneumonectomy thoracic cavity and the evaluation of the effect of slow releasing of basic fibroblast growth factor from a gelatin micro-sphere.

乳酸己内酯共聚物立方体在家兔肺切除术后胸腔内诱导造粒及明胶微球缓释碱性成纤维细胞生长因子的效果评价。

• 发表时间: 2005
• 期刊: ASAIO Journal (submitted)
• 作者: Noriyuki Misaki;Yasumichi Yamamoto;Taku Okamoto;Sung Soo Chang;Hitoshi Igai;Masashi Gotoh;Toshihiro Kushibiki;Yasuhiko Tabata;Hiroyasu Yokomise
• 通讯作者: Hiroyasu Yokomise

Bioabsorbable artificial trachea with slow release of bone morphogenetic proenin (BMP)-2 : a comparative evaluation of two different suturine techniques.

缓慢释放骨形态发生蛋白（BMP）-2的生物可吸收人工气管：两种不同缝合技术的比较评估。

• 期刊: ASAIO Journal, in 19 May 2005 (submitted)
• 作者: Yasumichi Yamamoto;Sung Soo Chang;Hitoshi Igai et al.
• 通讯作者: Hitoshi Igai et al.

Cartilage regeneration using slow release of bone morphogenetic protein (BMP)-2 from a gelatin sponge to treat experimenttal canine tracheomalacia : a prelininary report

使用明胶海绵缓慢释放骨形态发生蛋白（BMP）-2来治疗实验性犬气管软化症的软骨再生：初步报告

• 发表时间: 2003
• 期刊: ASAIO Journal 49
• 作者: Taku Okamoto;Yasumichi Yamamoto;Masashi Gotoh et al.
• 通讯作者: Masashi Gotoh et al.