权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The experimental study of the newly developed empyema treatment using self-tissue regeneration

新开发的自组织再生治疗脓胸的实验研究

基本信息

批准号：
17390383
负责人：
YOKOMISE Hiroyasu
金额：
$ 7.81万
依托单位：
Kagawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

We have tried to close the post-operative pleural dead space using biodegradable materials. The objective of this study was induction of granulation tissue in uninfected pleural space after pneumonectomy in a rabbit model using implantation of bioabsorbable and porous poly-L-lactic acid and ε-caprolactone copolymer (PLAC) cubes. Moreover, we examined whether the gelatin microspheres releasing bFGF would accelerate the induction of regeneration of host tissue. We have also investigated that the Cefmetazol could be released slowly from PLGA microspheres. In the control group, the parietal pleura were thickened and granulation tissue formation was not observed. In the experimental group, granulation tissue inside the PLAC cubes had begun to form at1month after implantation. At 3 months, proliferated granulation tissue occupied the left post-pneumonectomy pleural space with no residual space. The implanted PLAC material was being gradually degraded at 6 months and completely absorbed at 24 months. There is no acceleration of the regeneration adding the bFGF releasing gelatin microspheres sprinkling. We were able to induce self-assembled granulation tissue in the pleural space after pneumonectomy in a rabbit model using bioabsorbable PLAC cubes as the prombage material. The use of this technique, allowed the residual pleural space to be closed after pulmonary resection. The PLGA microspheres could release their inner Cefmetazol for more than 16 days in PBS solution. This PLGA microspheres including Cefmetazol might be useful for treatment of empyema.

我们尝试使用生物可降解材料关闭术后胸膜死腔。本研究的目的是通过植入生物可吸收和多孔聚-L-乳酸和ε-己内酯共聚物（PLAC）立方体，在兔模型肺切除术后未感染胸膜腔中诱导肉芽组织。此外，我们研究了释放bFGF的明胶微球是否会加速宿主组织的再生诱导。我们还研究了头孢美唑在PLGA微球中的缓释作用。对照组壁层胸膜增厚，未见肉芽组织形成。在实验组中，植入后1个月，PLAC立方体内的肉芽组织开始形成。3个月时，增生的肉芽组织占据了左肺切除术后的胸膜空间，没有残留空间。植入的PLAC材料在6个月时逐渐降解，并在24个月时完全吸收。添加释放碱性成纤维细胞生长因子的明胶微球喷洒并没有加速再生。我们能够诱导自组装肉芽组织在胸膜腔后，肺切除术在兔模型中使用生物可吸收的PLAC立方体作为prombage材料。该技术的使用允许在肺切除术后闭合残余胸膜间隙。PLGA微球在PBS溶液中可持续释放头孢美唑16天以上。这种含头孢美唑的PLGA微球可能用于脓胸的治疗。