An experimental study on circulatory physiology of nonpulsatile bloodcirculation under awake condition

虽然非搏动性循环在临床上被广泛使用，并且通常被认为是可行的，但脱髓哺乳动物的病理生理学还没有被清楚地了解。本研究旨在探讨非搏动性循环的循环生理学，以尽量减少不良额外因素的影响。首先，我们使用离心式连续流动血泵研究了成年山羊在清醒状态下对非搏动性体循环或肺循环的生理反应。在血液动力学、代谢、血管活性荷尔蒙和外周循环状况方面，体循环停滞并未显示出任何明显的不良影响。肺循环的解除也不影响肺循环状况和肺功能。根据这些结果，我们得出结论，清醒的哺乳动物立即适应无脉搏的体循环或肺循环。REM…在第一个系列研究中，更令人费解的是哺乳动物如何适应无脉动的循环。因此，在第二项研究中，我们使用成年山羊进行了急性动物实验，观察了神经和体液控制系统对非搏动性循环的急性时相反应。去皮后肾交感神经活动(RSNA)脉搏同步爆发即刻消失，平均RSNA增加120%。在评估的各种血管活性激素水平中，只有去甲肾上腺素在去皮后显著增加，平均动脉压的搏动性-非搏动性差异的幅度与去甲肾上腺素水平之间存在显著的负相关。结果表明，以去甲肾上腺素水平为代表的交感神经系统在非搏动性循环中的活性高于搏动性循环，压力感受器反射在体循环剥脱后的急性期起作用。较少

Although nonpulsatile circulation is widely used in the clinical setting and is generally conceived to be feasible, pathophysiology of depulsated mammals has not yet been clearly understood. The present study was undertaken to inquire circulatory physiology of nonpulsatile circulation in an effort to minimize the influence of untoward extra factors. First, we investigated physiologic responses of adult goats to nonpulsatile systemic or pulmonary circulation under awake condition in a total left or right heart bypass model using centrifugal-type continuous flow blood pump. Depulsation of systemic circulation did not show any significant adverse effects in hemodynamic, metabolic, vasoactive hormonal, and peripheral circulatory conditions. Depulsation of pulmonary circulation also did not affect pulmonary circulatory conditions as well as pulmonary functions. From these results, we concluded that an awake mammal acclimates immediately to nonpulsatile systemic or pulmonary circulation. Rem … More ained obscure in this first series study was how does the mammal acclimate to nonpulsatile circulation. In the second study, therefore, we looked into acute phase responses of the nervous and humoral control systems to nonpulsatile circulation in acute animal experiments of total left heart bypass model using adult goats. After the depulsation, pulse-synchronized bursts of the renal sympathetic nerve activity (RSNA) disappeared immediately and the mean RSNA increased up to 120%. Among various vasoactive hormone levels evaluated, only norepinephrine showed significant increase after the depulsation, and a significant reciprocal correlation was found between the magnitude of pulsatile-nonpulsatile differences in mean aortic pressure and norepinephrine levels. From these results, we concluded that activity of the sympathetic system represented by norepinephrine level is higher in nonpulsatile than pulsatile circulation, and the baroreceptor reflex functions in acute phase after the depulsation of systemic circulation. Less