An experimental study on circulatory physiology of nonpulsatile bloodcirculation under awake condition

虽然非搏动性循环在临床上被广泛应用，并且通常被认为是可行的，但对无搏动哺乳动物的病理生理学还没有清楚的了解。本研究旨在探讨无搏动循环的循环生理学，以减少外界不利因素的影响。首先，我们研究了成年山羊的生理反应，以清醒状态下的非脉动体循环或肺循环在全左或右心转流模型使用离心式连续血泵。体循环去搏动对血液动力学、代谢、血管活性激素和外周循环状况没有任何显著的不良影响。肺循环去搏动也不影响肺循环条件以及肺功能。从这些结果，我们得出结论，清醒的哺乳动物立即适应非脉动的体循环或肺循环。REM ...更多信息 在第一个系列研究中，哺乳动物是如何适应非脉动循环的还不清楚。因此，在第二项研究中，我们研究了急性期的神经和体液控制系统的反应，以非脉动循环在急性动物实验的全左心转流模型，使用成年山羊。去搏后，脉冲同步爆发的肾交感神经活动（RSNA）立即消失，平均RSNA增加到120%。在评价的各种血管活性激素水平中，只有去甲肾上腺素在去搏后显示出显著增加，并且发现平均主动脉压的脉动-非脉动差异的幅度与去甲肾上腺素水平之间存在显著的倒数相关性。结果表明，以去甲肾上腺素水平为代表的交感神经系统活性在非搏动性循环中高于搏动性循环，压力感受性反射在体循环去搏动后的急性期起作用。少

Although nonpulsatile circulation is widely used in the clinical setting and is generally conceived to be feasible, pathophysiology of depulsated mammals has not yet been clearly understood. The present study was undertaken to inquire circulatory physiology of nonpulsatile circulation in an effort to minimize the influence of untoward extra factors. First, we investigated physiologic responses of adult goats to nonpulsatile systemic or pulmonary circulation under awake condition in a total left or right heart bypass model using centrifugal-type continuous flow blood pump. Depulsation of systemic circulation did not show any significant adverse effects in hemodynamic, metabolic, vasoactive hormonal, and peripheral circulatory conditions. Depulsation of pulmonary circulation also did not affect pulmonary circulatory conditions as well as pulmonary functions. From these results, we concluded that an awake mammal acclimates immediately to nonpulsatile systemic or pulmonary circulation. Rem … More ained obscure in this first series study was how does the mammal acclimate to nonpulsatile circulation. In the second study, therefore, we looked into acute phase responses of the nervous and humoral control systems to nonpulsatile circulation in acute animal experiments of total left heart bypass model using adult goats. After the depulsation, pulse-synchronized bursts of the renal sympathetic nerve activity (RSNA) disappeared immediately and the mean RSNA increased up to 120%. Among various vasoactive hormone levels evaluated, only norepinephrine showed significant increase after the depulsation, and a significant reciprocal correlation was found between the magnitude of pulsatile-nonpulsatile differences in mean aortic pressure and norepinephrine levels. From these results, we concluded that activity of the sympathetic system represented by norepinephrine level is higher in nonpulsatile than pulsatile circulation, and the baroreceptor reflex functions in acute phase after the depulsation of systemic circulation. Less