A role of nitric oxide in regulation of the cerebral microcirculation

一氧化氮对脑微循环的调节作用

• 批准号: 06671384
• 负责人: TAKAYASU M
• 金额: $ 1.28万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671384/
• 关键词: Cerebral arterioles; Nitric oxide; Cerebral microcirculation; N^G-monomethyl-L-arginine; L-arginine; Oxyhemoglobin; Vasopressin; 一酸化窒素(NO); 脳実質内細動脈; L-NMMA

Nitric oxide (NO) is a potent vasodilator that plays a substantial role in the regulation of cerebral circulation. However, little is known about its effects on the cerebral microcirculation. Therefore, we investigated a role of NO in regulation of the cerebral microcirculation, using isolated intracerebral arterioles from rats. The arterioles were cannulated on the stage of the inverted microscope and pressurized to 60 mmHg. The inner diameter was determined by a video micro-scaler. At first, regulation of basal tone of the arterioles by NO was examined using NO inhibitor, N^G-monomethyl-L-arginine (L-NMMA) and substrate of NO,L-arginine. L-NMMA (10^<-3>M) produced vasoconstriction of 14% in arterioles originated from the middle cerebral artery, and 23% in arterioles from the basilar artery. On the other hand, L-arginine (10^<-3>M) produced vasodilatation of 11% in arterioles originated from the middle cerebral artery, and 24% in arterioles from the basilar artery. These findings sugg … More est that the roles of NO in vasomotor control differ by regions in the brain, and it may be greater in vessels of the posterior than of the anterior circulation. Next, as a model of the pathological condition, the arterioles were pretreated with oxyhemoglobin (OxyHb) and vasopressin, an endogenous vasoactive substance, was applied to the arterioles. Vasopressin produced a triphasic, dose-dependent response consisting of casodilation (10^<-11>M), vasoconstriction (10^<-9>-10^<-8>M) and a decrease in casoconstriction (10^<-7>-10^<-6>M). Pretreatment with OxyHb (10^<-4>M) abolished the vasodilation induced by the lower dose of vasopressin and doubled the vasoconstriction induced by the higher dose. These results suggest that nitric oxide play an important roles in regulation of the cerebral microciriculation directly and indirectly through the effects of the other vasoactive substances both in the physiological and pathological conditions. Finally we attempted to measure the levels of NO,which was released from the isolated and cannulated single arteriole after administration of L-arginine in vitro. However, No could not be detected in this moment because the mechanical noise level seemed much higher than the level of NO. Less

一氧化氮（NO）是一种有效的血管扩张剂，在脑循环调节中起着重要作用。然而，人们对其对大脑微循环的影响知之甚少。因此，我们利用离体大鼠脑内小动脉研究一氧化氮在脑微循环调节中的作用。在倒置显微镜下插管，并加压至60 mmHg。内径由视频微型秤测定。首先，采用NO抑制剂N^ g -单甲基- l-精氨酸（L-NMMA）和NO底物l-精氨酸检测NO对小动脉基底电位的调节作用。L-NMMA （10^<-3>M）使大脑中动脉动脉血管收缩14%，基底动脉动脉血管收缩23%。另一方面，l -精氨酸（10^<-3>M）在大脑中动脉的小动脉中产生11%的血管扩张，在基底动脉的小动脉中产生24%的血管扩张。这些发现表明，一氧化氮在血管舒缩控制中的作用在大脑的不同区域有所不同，在后循环血管中的作用可能大于前循环血管。接下来，作为病理状态的模型，用氧合血红蛋白（OxyHb）和血管加压素（一种内源性血管活性物质）预处理小动脉。抗利尿激素产生一个剂量依赖性的三期反应，包括caso舒张（10^<-11>M）、血管收缩（10^<-9>-10^<-8>M）和caso收缩减少（10^<-7>-10^<-6>M）。预处理氧合血红蛋白（10^<-4>M）可消除低剂量抗利尿素引起的血管舒张，使高剂量抗利尿素引起的血管收缩增加一倍。这些结果表明，在生理和病理条件下，一氧化氮通过其他血管活性物质的作用，直接或间接地调节大脑微循环。最后，我们尝试测量l -精氨酸在体外给药后，从离体和插管的单小动脉中释放的一氧化氮水平。然而，此时无法检测到No，因为机械噪声水平似乎比No高得多。少

项目成果

Takayasu M,Kajita Y,Suzuki Y,et.al.: "Oxyhemoglobin enhancement of vasopressininduced constriction in rat cerebral arterioles." Life Sciences. 56. PL123-127 (1995)

Takayasu M、Kajita Y、Suzuki Y 等人：“氧合血红蛋白增强加压素诱导的大鼠脑小动脉收缩。”

Kajita Y,Takayasu M,Suzuki Y,et al.: "Regional differences in cerebral vasomotor control by nitric oxide" Brain Res Bull. 38. 365-369 (1995)

Kajita Y、Takayasu M、Suzuki Y 等人：“一氧化氮控制脑血管舒缩的区域差异”Brain Res Bull。

Takayasu M,Kajita Y,Suzuki Y,et.al.: "A role of nitric oxide in vasomotor control of cerebral parenchymal arterioles in rats." J Autonom Nerv Syst. 49. S63-S66 (1994)

Takayasu M、Kajita Y、Suzuki Y 等人：“一氧化氮在大鼠脑实质小动脉血管舒缩控制中的作用。”

Takayasu M,Kajita Y,et al: "Oxyhemoglobin enhancemnt of vasopressin-induced constriction in rat cerebral artevioles." Life Sciences. 56. ┣D1PL┫D1123-127 (1995)

Takayasu M、Kajita Y 等人：“氧合血红蛋白增强加压素诱导的大鼠脑动脉收缩。”56。┣D1PL┫D1123-127 (1995)

Takayasu M,Kajita Y,Suzuki Y,et al: "Oxyhemoglobin enhancement of vasopressin-induced constiction in rat cerebral arterioles" Life Science. Vol.56-No.5. PL123-127 (1995)

Takayasu M、Kajita Y、Suzuki Y 等人：“氧合血红蛋白增强加压素诱导的大鼠脑小动脉收缩”生命科学。