Assessment of analgesic effect of general anesthetics by gene expression (c-fos)

通过基因表达评估全身麻醉药的镇痛效果（c-fos）

• 批准号: 06671518
• 负责人: NAKAO Shin-ichi
• 金额: $ 1.34万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671518/
• 关键词: c-fos; halothane; pain; spinal cord; formalin; pain

In the present study, the effect of halothane on formalin-induced c-fos protein expression in rat spinal cord was investigated.All rats received 5% formalin into the unilateral hind paws and then were randomly assigned into 5 groups as follows : (1) Control group. (2) The rats were continuously insufflated with 1.1% halothane. (3) The rats were continuously insufflated with 1.7% halothane. (4) The rats were insufflated with 1.7% halothane only during the phase 1 response to formalin. (5) The rats were insufflated with 1.7% halothane only during the phase 2 response to formalin.Halothane suppressed c-fos protein expression in a dose dependent manner. The c-fos protein expression of not only group 4 but also group 5 rats was significantly reduced compared with that of control rats. However, the magnitude of these reductions was significantly less than that of group 3 rats.The data confirm that halothane suppresses the spinal neuronal response to noxious stimulation. They also suggest that halothane is able to suppress both the induction and maintenance of central sensitization. The maximum suppression, however, may be obtained in conditions when halothane is administered both before and after the establishment of central sensitization by noxious stimulation.

本实验研究了氟烷对福尔马林诱导的大鼠脊髓c-fos蛋白表达的影响。取大鼠单侧后爪注射5%福尔马林，随机分为5组：(1)对照组。(2)大鼠连续灌胃1.1%氟烷。(3)大鼠连续灌胃1.7%氟烷。(4)大鼠在福尔马林第1期反应时只注射1.7%氟烷。(5)在福尔马林第2期反应期间，大鼠只注射1.7%氟烷。氟烷抑制c-fos蛋白表达呈剂量依赖性。与对照大鼠相比，不仅4组大鼠，5组大鼠的c-fos蛋白表达均显著降低。然而，这些减少的幅度明显小于3组大鼠。数据证实，氟烷抑制脊髓神经元对有害刺激的反应。他们还表明，氟烷能够抑制中枢致敏的诱导和维持。然而，在通过有害刺激建立中枢致敏之前和之后同时施用氟烷的条件下，可能获得最大的抑制。

项目成果

Takehiko Adachi: "Nitric oxide synthetase inhibitor does not reduce minimum alveolar anesthetic concentration of halothane in rats" Anesthesia Analgesia. 78. 1154-1157 (1994)

Takehiko Adachi：“一氧化氮合成酶抑制剂不会降低大鼠氟烷的最低肺泡麻醉浓度”麻醉镇痛。

J Kurata, S Nakao, M Murakawa, T Adachi, T Shichino, K Mori: "The cerebral cortex origin ofenflurane-induced generalized seizures in cats." Anesth Analg. 79. 713-718 (1994)

J Kurata、S Nakao、M Murakawa、T Adachi、T Shichino、K Mori：“安氟烷引起的猫全身性癫痫发作的大脑皮层起源。”

S Nakao, J Kurata, T Arai, M Murakawa, T Adachi, MN Avramov, K Mori, O Yasuhara, I Tooyama, H Kimura: "Lignocaine-induced convuision does not induce c-fos protein (c-Fos) in rat hippocampus." Acta Anaesthesiol Scand. 38. 845-851 (1994)

S Nakao、J Kurata、T Arai、M Murakawa、T Adachi、MN Avramov、K Mori、O Yasuhara、I Tooyama、H Kimura：“利多卡因诱导的惊厥不会在大鼠海马中诱导 c-fos 蛋白 (c-Fos)

T Arai, K Watanabe, S Nakao, H Mori, M Murakawa, K Mori, I Tooyama, H Kimura, S Kojima: "Effects of neopterin on ischemic neuronal damage in gerbils." Neurosci Lett. 173. 107-110 (1994)

T Arai、K Watanabe、S Nakao、H Mori、M Murakawa、K Mori、I Tooyama、H Kimura、S Kojima：“新蝶呤对沙鼠缺血性神经元损伤的影响。”

Shin-ichi Nakao: "High-dose ketamine does not induce c-Fos protein expression in rat hippocampus" Neuroscience Letters. 151. 33-36 (1993)

Shin-ichi Nakao：“高剂量氯胺酮不会诱导大鼠海马中的 c-Fos 蛋白表达”《神经科学快报》。