Development of processing techniques for liposomes using membranes

开发利用膜的脂质体加工技术

• 批准号: 23656496
• 负责人: NAKAO Shin-ichi
• 金额: $ 2.5万
• 依托单位: Kogakuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23656496/
• 关键词: リポソーム; SPG 膜; 脂質; 単分散; 流動性; アルコール; SPG膜; DDS

In this study, we developed a facile and novel method for preparing liposomes simply by permeating lipid-alcohol solutions through Shirasu porous glass (SPG) membranes.This is based on the phenomenon in which an interface between a lipid-alcohol solution and water is formed and the two solutions mix with each other at the surface of an SPG membrane when the lipid-alcohol solution is slowly permeated through the membrane; this reduces the concentration of the alcohol solution and induces spontaneous formation of liposomes. We successfully prepared 100-nm monodispersed liposomes by this method using lipid-isopropyl alcohol (IPA) and lipid-ethanol (EtOH) solutions. Additionally, an increase in the liposome size was clearly observed in freezing-thawing treatment when IPA was used as the alcohol, whereas the liposome size was unchanged by freezing-thawing treatment when EtOH was used. It is interesting that the alcohol affected the liposome properties, in particular the membrane fluidity, even although no effect of the alcohol on the liposome structure, such as size, monodispersity, was observed.

本研究利用脂质-乙醇溶液缓慢透过多孔玻璃膜时，脂质-乙醇溶液与水在多孔玻璃膜表面形成界面，两种溶液在膜表面混合的现象，发展了一种简便、新颖的脂质体制备方法，即脂质-乙醇溶液透过多孔玻璃膜;这降低了醇溶液的浓度并诱导脂质体的自发形成。我们成功地制备了100 nm的单分散脂质体通过这种方法使用脂质异丙醇（IPA）和脂质乙醇（EtOH）的解决方案。此外，当使用IPA作为醇时，在冻融处理中清楚地观察到脂质体尺寸的增加，而当使用EtOH时，脂质体尺寸未被冻融处理改变。有趣的是，醇影响脂质体性质，特别是膜流动性，即使没有观察到醇对脂质体结构如大小、单分散性的影响。