EVALUATION OF EFFECT OF SODIUM PENTOSAN POLYSULFATE (SPP) AND CITRATE ON STONE FORMATION IN HYPEROXALURIC RATS BY RADIOLUMINOGRAPHY USING ^<14>C-OXALATE

使用^ 14 C-草酸盐通过放射发光照相法评价戊聚糖多硫酸钠(SPP)和柠檬酸盐对高草酸大鼠结石形成的影响

• 批准号: 06671608
• 负责人: YAMAMOTO Keisuke
• 金额: $ 1.15万
• 依托单位: OSAKA CITY UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671608/
• 关键词: KIDNEY STONE; CALCIUM OXALATE STONE; OXALIC ACID; AUTORADIOGRAPHY; VITAMIN B6 DEFICIENCY; CITRATE; SODIUM PENTOSAN POLYSULFATE; ビタミンD6欠乏

Using radioluminographic techniques, we studied the effect of sodium pentosan polysulfate (SPP) and citrate on calcium oxalate stone formation in hyperoxaluric rats induced by feeding vitamin B6 deficient diet. Hyperoxaluric rats were divided into four groups. Rats in the first group were given only tap water as the control. Rats in the second group (SPP 2-4w group) had no administration for 2 weeks and after that time were injected with 10mg SPP every other day for 2 weeks. The third group (SPP 0-4w group) received the same dose SPP every other day for 4 weeks. And rats in the last troup (Cit group) were maintained with 60mM citrate in tap water for 4 weeks. At 4 weeks all rats were injected with ^<14>C-oxalate intravenously (7.4MBq/kg) and 18 hours after the injection they were killed and immediately nephrectomized bilaterally. Kidneys were sliced 40mum in thickness using a microtome. Each kidney specimen was directly in contact with an imaging plate. The radioactivity of ^<14>C-oxal … More ate in each imaging plate was measured by photostimulated luminescence method. Radioactivity in cach kidney slice could be measured more accurately by radioluminographic techniques than conventional autoradiographic studies. Moreover, the radioactivity measured by radioluminography correlated excellently with that of liquid scintillation counter method. Ten radioimmunograms were analyzed in each animal and both the total number and total detected radioactivity of deposits of ^<14>C-oxalate were calculated. The maximum radioactivity of deposits in each rat was also measured.In control group, deposits of ^<14>C-oxalate which indicated calcium oxalate stone formation were induced in the renal papilla. In the other three groups, the total number and total detected radioactivity of deposits of ^<14>C-oxalate were significantly decreased in comparison with the control. The maximum radioactivity of deposits in these three groups was significantly lower than that of the control group, respectively. These results indicate that both SPP and citrate must be effective inhibitory agents on urinary stone formation. Less

采用放射荧光技术，研究了戊聚糖聚硫酸钠（SPP）和柠檬酸钠对维生素B6缺乏饮食诱导的高血氧大鼠草酸钙结石形成的影响。将高血氧大鼠分为四组。第一组大鼠只喝自来水作为对照。第二组（SPP 2-4w组）大鼠不给药2周后，每隔一天注射SPP 10mg，连续2周。第三组（SPP 0 ~ 4w组）每隔一天给予相同剂量的SPP，连续4周。最后一组（Cit组）给予60mM枸橼酸自来水维持4周。4周时，所有大鼠静脉注射<14>的草酸c (7.4MBq/kg)，注射后18小时处死，立即双侧肾切除。肾用切片机切片40mm厚。每个肾脏标本直接与成像板接触。用光激发发光法测定了各成像板上^<14>C-oxal…More ate的放射性。与传统的放射自显影研究相比，放射成像技术可以更准确地测量每片肾片中的放射性。此外，用放射发光法测得的放射性与液体闪烁计数器法测得的放射性有很好的相关性。分析了每只动物的10张放射免疫图，计算了^<14> c -草酸盐沉积物的总数和总检测放射性。还测量了每只大鼠体内沉积物的最大放射性。对照组肾乳头内可见^<14> c -草酸沉积，提示形成草酸钙结石。在其他3组中，与对照组相比，^<14> c -草酸盐沉积物的总数和总检测放射性均显著降低。三组沉积物的最大放射性均显著低于对照组。这些结果表明SPP和柠檬酸盐都是抑制尿路结石形成的有效药物。少

项目成果

T.Kanazawa: "Macro and micro autoradiographic studies on oxalate in normal and hyperoxaluric rats." Urolithiasis 2 Plenum,New York,1994. 113-115 (1994)

T.Kanazawa：“正常和高草酸尿大鼠草酸盐的宏观和微观放射自显影研究。”

S.Kamikawa: "Autoradiographic study on calcium oxalate crystal fixation in rat kidney." Urolithiasis Consensus and Controversis. 429-430 (1995)

S.Kamikawa：“大鼠肾脏中草酸钙晶体固定的放射自显影研究。”

T.Kanazawa: "Macro and micro autoradiographic studies on oxalate in normal and hyperoxaluria rats." Urolithiasis 2. 113-115 (1994)

T.Kanazawa：“正常和高草酸尿症大鼠草酸盐的宏观和微观放射自显影研究。”

T.Kanazawa: "Macro and micro autoradiographic studies on oxalate in normal and hyperoxaluric rats." Urolithiasis 2 Plenum,New York. 113-115 (1994)

T.Kanazawa：“正常和高草酸尿大鼠草酸盐的宏观和微观放射自显影研究。”

上川禎則: "オートラジオグラフィーによる蓚酸の腎内分布の解析" 日本泌尿器科学会雑誌. (投稿中).

Sadanori Kamikawa：“放射自显影分析肾内草酸盐分布”，日本泌尿外科协会杂志（目前正在提交）。