EVALUATION OF EFFECT OF SODIUM PENTOSAN POLYSULFATE (SPP) AND CITRATE ON STONE FORMATION IN HYPEROXALURIC RATS BY RADIOLUMINOGRAPHY USING ^<14>C-OXALATE

本文用放射发光技术研究了戊聚糖多硫酸钠（SPP）和柠檬酸盐对维生素B_6缺乏引起的高尿酸大鼠草酸钙结石形成的影响。高尿酸大鼠分为4组。第一组大鼠仅给予自来水作为对照。第二组（SPP 2- 4 w组）停药2周后隔日注射SPP 10 mg，共2周。第三组（SPP 0- 4 w组）每隔一天给予等剂量SPP，共4周。最后一组大鼠（Cit组）用60 mM柠檬酸自来水维持4周。在4周时，所有大鼠静脉注射14 <14>C-草酸盐（7.4MBq/kg），并且在注射后18小时，将它们处死并立即双侧肾切除。使用切片机将肾脏切成40 μ m厚。每个肾脏标本直接与成像板接触。^<14>C-草醛的放射性 ...更多信息 通过光激励发光法测量每个成像板中的ATE。放射性发光技术比传统的放射自显影研究能更准确地测量每个肾切片中的放射性。放射发光法与液体闪烁计数法测定的放射性具有良好的相关性。分析每只动物的10个放射免疫图，并计算14 C-草酸盐沉积物的总数和总检测放射性<14>。对照组大鼠<14>肾乳头内可见草酸钙结石形成的沉积物，即~（13）C-草酸盐沉积。在其他三组中，与对照组相比，沉积物的总数量和总检测到的放射性<14>显著降低。三组沉积物的最大放射性均显著低于对照组。这些结果表明，SPP和柠檬酸盐必须是有效的抑制剂对尿路结石的形成。少

Using radioluminographic techniques, we studied the effect of sodium pentosan polysulfate (SPP) and citrate on calcium oxalate stone formation in hyperoxaluric rats induced by feeding vitamin B6 deficient diet. Hyperoxaluric rats were divided into four groups. Rats in the first group were given only tap water as the control. Rats in the second group (SPP 2-4w group) had no administration for 2 weeks and after that time were injected with 10mg SPP every other day for 2 weeks. The third group (SPP 0-4w group) received the same dose SPP every other day for 4 weeks. And rats in the last troup (Cit group) were maintained with 60mM citrate in tap water for 4 weeks. At 4 weeks all rats were injected with ^<14>C-oxalate intravenously (7.4MBq/kg) and 18 hours after the injection they were killed and immediately nephrectomized bilaterally. Kidneys were sliced 40mum in thickness using a microtome. Each kidney specimen was directly in contact with an imaging plate. The radioactivity of ^<14>C-oxal … More ate in each imaging plate was measured by photostimulated luminescence method. Radioactivity in cach kidney slice could be measured more accurately by radioluminographic techniques than conventional autoradiographic studies. Moreover, the radioactivity measured by radioluminography correlated excellently with that of liquid scintillation counter method. Ten radioimmunograms were analyzed in each animal and both the total number and total detected radioactivity of deposits of ^<14>C-oxalate were calculated. The maximum radioactivity of deposits in each rat was also measured.In control group, deposits of ^<14>C-oxalate which indicated calcium oxalate stone formation were induced in the renal papilla. In the other three groups, the total number and total detected radioactivity of deposits of ^<14>C-oxalate were significantly decreased in comparison with the control. The maximum radioactivity of deposits in these three groups was significantly lower than that of the control group, respectively. These results indicate that both SPP and citrate must be effective inhibitory agents on urinary stone formation. Less