CRYSTAL CELL INTERACTION BETWEEN RENAL EPITHELIAL CELLS AND CALCIUM OXALATE CRYSTALS USING THE ANIMAL MODEL FOR CALCIUM OXALATE STONE

在2001年，进行了一项研究，以建立一个模型，由草酸钙诱导的肾结石，这将是适合于研究草酸钙晶体和肾上皮之间的晶体-细胞相互作用。在该研究中，通过调整常规体内实验中使用的条件，有可能确定可以诱导草酸钙晶体形成的条件，同时使肾损伤最小化。更具体地说，似乎适合给予0.1- 0.2%乙二醇加1.0%氯化铵9天或单独给予0.4%乙二醇14天。除了在体外进行的晶体-细胞相互作用的研究外，这些条件的确定使体内实验成为可能。2002年，进行了以下两项实验。实验1：草酸钙晶体暴露于肾集合管细胞的细胞膜的研究。在诱导草酸钙晶体形成的条件下， ...更多信息 用流式细胞仪和荧光显微镜观察肾集合管细胞膜上磷脂酰丝氨酸的分布。证实了表达磷脂酰丝氨酸的细胞随着草酸盐和草酸钙晶体的尿排泄而逐渐增加。凋亡信号转导系统之一的Caspase家族也被激活。实验2：多硫酸软骨素对草酸钙晶体与肾上皮细胞粘附的抑制作用。当在与实验1相同的条件下用多硫酸软骨素处理大鼠时，与未处理的对照组相比，肾集合管细胞上的磷脂酰丝氨酸暴露和半胱天冬酶3、8和9的活性倾向于被抑制。这些结果表明，轻度细胞损伤对晶体-细胞相互作用的影响可以通过体内实验定量分析。草酸钙晶体引起的肾集合管细胞的小损伤似乎可以被多硫酸软骨素控制，从而抑制结石形成。少

In 2001, a study was conducted to establish a model of renal stones induced by calcium oxalate that would be suitable for investigating crystal-cell interactions between calcium oxalate crystals and renal epithelium. In that study, it was possible to determine conditions under which calcium oxalate crystaluria can be induced while minimizing renal injury, by adjusting the conditions used in conventional in vivo experiments. More specifically, it seemed to be suitable to administer 0.1-0.2 % ethylene glycol plus 1.0 % ammonium chloride for 9 days or 0.4 % ethylene glycol alone for 14 days. In addition to the studies of crystal-cell interactions conducted in vitro, determination of these conditions made it possible to endorse in vivo experiment.In 2002, the following two experiments were carried out.Experiment 1: A study on the cell membrane of the renal collecting duct cells exposed to calcium oxalate crystals. Under conditions inducing the formation of calcium oxalate crystals, changes … More in the distribution of phosphatidylserine on the cell membrane of the renal collecting duct cells were examined by flowcytometry and fluorescent microscope. It was confirmed that phosphatidylserine expressing cells gradually increased with urinary excretion of oxalate and calcium oxalate crystals. Caspase family, one of the intracellular signal transduction systems for apoptosis, was also activated.Experiment 2: Effects on chondroitin polysulfate to prevent the adhesion of calcium oxalate crystals to the renal epithelial cells. When rats were treated with chondroitin polysulfate under the same conditions as Experiment 1, phosphatidylserine exposure on the renal collecting duct cells and the activities of caspase 3, 8, and 9 tended to be suppressed comparing to the untreated control groups. These results indicate that the effects of mild cell injury on crystal-cell interactions can be quantitatively analyzed by in vivo experiment. It seems likely that the small injury of the renal collecting duct cells caused by calcium oxalate crystals can be controlled by chondroitin polysulfate, leading to suppression of stone formation. Less