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Effects of PBSC reinfusion on tumor immunity in patients with urologic malignancies

PBSC回输对泌尿系统恶性肿瘤患者肿瘤免疫的影响

基本信息

批准号：
06671612
负责人：
SUWATA Junji
金额：
$ 1.41万
依托单位：
Saitama Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671612/
关键词：
Periperal blood stem cells CD34 positive cells Colony formation assay Flow cytometry Macrophages CD69 Cytokines Tumorimmunology PBSC 腫瘍免疫泌尿器科悪性腫瘍

项目摘要

Peripheral blood progenitor cells (PBSC) reinfusion has contributed to the advance of myeloablative chemotherapy in patients with urologic cancers as well as hematological malignancies. Virtually colony forming cells as progenitors in human bone marrow and peripheral blood can be identified by the expression of the CD34 antigen. It is commonly believed that number of CD34 positive cells in PBSC reinfusion have been correlated with hematopoieticrestore. Using flow cytometric analysis, first we established a technique for detecting CD34 positive cells' concentration in peripheral blood and PBSC harvesting cells (published in Biothrapy). Our technique for detecting CD34 positive cells had been well established and correlated with the results of colony formation assay (published in Igakukensa).On the other hands, PBSC harvested cells contained a number of macrophages. It was recently reported the such macrophages strongly secleted cytokines for stimulating hematopoiesis. We secondly investigated on the effect of PBSC reinfusion to the immune-modulation in patients. CD69 antigen that is an early activating antigen and also an antigen related with NK-gene family complex was identified by dual color flow cytometric analysis on cytokine seclated circulating monocytes in PBSC harvesting cells (ISBT). We confirmed change in CD69 antigen expression to be a good indicator of PBSC moblization (ISBT). However, before this immunological issue become clear, it seemed further investigations will be required. In conclusion, our establishment technique for detecting CD34 positive cells in peripheral blood and PBSC harvesting cells is well established. Change in CD69 antigen expression on circulated monocytes might demonstrate some immunological reaction in patients.

外周血祖细胞（PBSC）回输促进了泌尿系统癌症和血液恶性肿瘤患者清髓化疗的进展。实际上，可以通过 CD34 抗原的表达来鉴定作为人骨髓和外周血中的祖细胞的集落形成细胞。人们普遍认为，PBSC 回输中 CD34 阳性细胞的数量与造血恢复相关。使用流式细胞术分析，我们首先建立了一种检测外周血和PBSC收获细胞中CD34阳性细胞浓度的技术（发表于Biothrapy）。我们用于检测CD34阳性细胞的技术已经得到很好的建立，并且与集落形成测定（发表于Igakukensa）的结果相关。另一方面，PBSC收获的细胞含有大量巨噬细胞。最近报道这种巨噬细胞强烈分泌细胞因子来刺激造血。其次，我们研究了 PBSC 回输对患者免疫调节的影响。 CD69 抗原是一种早期激活抗原，也是一种与 NK 基因家族复合物相关的抗原，通过对 PBSC 收获细胞 (ISBT) 中细胞因子分泌的循环单核细胞进行双色流式细胞术分析来鉴定。我们证实 CD69 抗原表达的变化是 PBSC 动员 (ISBT) 的良好指标。然而，在这个免疫学问题变得清晰之前，似乎还需要进一步的研究。总之，我们检测外周血和 PBSC 收获细胞中 CD34 阳性细胞的建立技术已经很成熟。循环单核细胞上 CD69 抗原表达的变化可能表明患者存在一些免疫反应。