Gene expression analysis of bone marrow CD34 positive cells in patients with rheumatoid arthritis

类风湿关节炎(RA)BM CD34+细胞FKBP5的mRNA表达明显高于骨关节炎(OA)。用肿瘤坏死因子-α刺激RAW 264.7(小鼠白血病单核巨噬细胞系)稳定表达FKBP5，使其表达上调，并向成纤维细胞样细胞转化。这可能是RA患者骨髓和关节炎之间的一条可能的联系途径。发现FKBP5等与类风湿关节炎发病相关的基因，可为类风湿关节炎的基础基因治疗提供新的思路。

The expression of mRNA for FKBP5 was significantly higher in rheumatoid arthritis (RA) BM CD34+ cells than those in osteoarthritis (OA). FKBP5 stable transfectant of RAW 264.7 (Mouse leukemic monocyte macrophage cell line) was made and stimulated with TNF-α, resulted in up-regulation of NFkB and transformation to fibroblast-like cells. This is a possible pathway linking bone marrow and arthritis in RA patients. Identification of the gene associated with pathogenesis of RA such as FKBP5 can lead to new fundamental gene therapy of RA.