Gene expression analysis of bone marrow CD34 positive cells in patients with rheumatoid arthritis

类风湿关节炎（RA）骨髓CD 34+细胞中FKBP 5 mRNA的表达显著高于骨关节炎（OA）。制备了FKBP 5稳定转染小鼠白血病单核巨噬细胞系RAW 264.7的转染子，并用TNF-α刺激，导致NF κ B上调并转化为成纤维细胞样细胞。这是一个可能的途径连接骨髓和关节炎的RA患者。发现与RA发病相关的基因，如FKBP 5，将为RA的基础基因治疗提供新的思路。

The expression of mRNA for FKBP5 was significantly higher in rheumatoid arthritis (RA) BM CD34+ cells than those in osteoarthritis (OA). FKBP5 stable transfectant of RAW 264.7 (Mouse leukemic monocyte macrophage cell line) was made and stimulated with TNF-α, resulted in up-regulation of NFkB and transformation to fibroblast-like cells. This is a possible pathway linking bone marrow and arthritis in RA patients. Identification of the gene associated with pathogenesis of RA such as FKBP5 can lead to new fundamental gene therapy of RA.