Study for treatment of head and neck cancer by the mechanism of a poptosis

通过细胞凋亡机制治疗头颈癌的研究

• 批准号: 06671742
• 负责人: KUMAZAWA Hirobumi
• 金额: $ 1.28万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671742/
• 关键词: apoptosis; chemotherapy; head and neck cancer; apoptosis; 頭頚部癌

Apoptosis is a specific mode of cell death characterized by morphological, biological and molecular changes. Cell death by apoptosis is common in normal tissues and with organ involution and in cytotoxic immunological reactions. This mode of cell death in neoplastic tissues has recently become a focus of interest. Since little is known of apoptosis inhead and neck tumors, we studied the morphological changes in head and neck tumor-derived cell lines (KB,KBrc, HSC-2,3,4) in the in vitro experimental assay. Furthermore, we tried to use the apoptotic reaction for overcoming CDDP resistance. In the in vivo model, which we developed using nude mouse model, we observed CDDP resistance after multiple application of 254-S.However, the apoptotic reaction did not overcome the resistance of 254-S in tumor cells.

细胞凋亡是一种以形态学、生物学和分子生物学变化为特征的细胞死亡方式。细胞凋亡导致的细胞死亡在正常组织、器官退化和细胞毒性免疫反应中很常见。肿瘤组织中的这种细胞死亡模式最近已成为关注的焦点。由于对头颈部肿瘤细胞凋亡的研究较少，本实验采用体外实验方法，研究了头颈部肿瘤细胞株KB、KBrc、HSC-2、3、4的形态学变化。此外，我们试图利用凋亡反应来克服CDDP抗性。在体内模型中，我们使用裸鼠模型开发，我们观察到CDDP耐药后，多次应用254-S。然而，凋亡反应并没有克服肿瘤细胞中的254-S的耐药性。

项目成果

Hirobumi Kumazawa: "Induction of apoptosis in head and neck tuwar cell lines by anti Fas antibody" Int J Oncol. 6. 753-758 (1995)

Hirobumi Kumazawa：“抗 Fas 抗体诱导头颈 tuwar 细胞系凋亡”Int J Oncol。

Hirobumi Kumazawa, Takuya Tachikawa, Shin-ichi Sai, Toshio Yamashita, Keiji Kawamoto, Tatsuki Oyaizu, Airo Tsubura.: "Induction of apoptosis in head and neck tumor cell lines by anti-Fas" Int J Oncol. 6. 753-758 (1995)

Hirobumi Kumazawa、Takuy​​a Tachikawa、Shin-ichi Sai、Toshio Yamashita、Keiji Kawamoto、Tatsuki Oyaizu、Airo Tsubura.：“抗 Fas 诱导头颈肿瘤细胞系凋亡”Int J Oncol。

Hirobumi Kumazawa, Ryouichi Kyoumoto, Ayumi Matsumoto, Kazumasa Baba, Kouichi Tomoda, Toshio Tachikawa.: "-Review- A severe combined immunodeficient mouse model for the in vivo study of human acoustic schwannoma" Am J Otolaryngol. 17. 228-232 (1996)

Hirobumi Kumazawa、Ryouichi Kyoumoto、Ayumi Matsumoto、Kazumasa Baba、Kouichi Tomoda、Toshio Tachikawa.：“-回顾-用于人声神经鞘瘤体内研究的严重联合免疫缺陷小鼠模型”Am J Otolaryngol。

Shin-ichi Sai: "Alteration of sensitivity to 254-Sin oral tumor xenograft" Int J Oncol. 8. 58-63 (1996)

Shin-ichi Sai：“对 254-Sin 口腔肿瘤异种移植物的敏感性的改变”Int J Oncol。

Hirobumi Kumazawa: "Local injection of OK-432/fibrinogen gel into heed and neck carcinomas" Evr J.Cancer. 30A. 1741-1744 (1995)

Hirobumi Kumazawa：“将 OK-432/纤维蛋白原凝胶局部注射到头颈癌中”Evr J.Cancer。