Effect of beta-carotene on epithelio-mesenchymal interaction during ocbntogenesis and oncogenesis in NMU-treated hamster

β-胡萝卜素对 NMU 处理的仓鼠在发病和肿瘤发生过程中上皮间质相互作用的影响

• 批准号: 06671800
• 负责人: KOHGO Takao
• 金额: $ 1.34万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671800/
• 关键词: Odontogenic tumor; beta-carotene; Disturbances in odontogenesis; Hamster; N-nitrosomethylurea; 上皮間葉相互作用; 歯方形成異常; N-nitrosomethylurea; 上皮-間葉相互作用

We have reported the induction of disturbances in odontogenesis and odontogenic tumors in incisors of NMU-treated hamsters, and also demonstrated the effects of vitamin A on tumorigenesis and disturbances in odontogenesis. In the present study, we investigated the effect of natural and synthetic beta-carotene on NMU-treated hamsters. Six hours after administration of beta-carotenes, NMU treatment was conducted twice a week for three months. The NMU-induced disturbances in the odontogenesis of mandibular incisors were characterized by hypoplastic changes ranging from enamel hypoplasia to loss of enamel and irregular hypoplastic dentin formation. In NMU-treated hamsters, excess administration of beta-carotene slightly repressed disturbances in odontogenesis, atypical proliferation of odontogenic epithelium, and keratinization of epithelial components. In NMU inhibited ameloblast differentiation, the differentiation on odontoblasts and cell to cell contact between preameloblasts and odontoblasts were not prevented. The beta-carotene did not prevent NMU-induced inhibition of ameloblast differentiation but inhibited NMU-induced keratinization of odontogenic epithelium. The beta-carotene had a moderate suppressive effect on the development of the odontogenic tumors. Synthetic beta-carotene seems to be slightly more effective on NMU-induced disturbances in odontogenesis than natural beta-carotene. The beta-carotene may play a role in the normal differentiation of odontogenic epithelial tissue as a biological function and as a provitamin A function. This study may suggest inhibitory effects of beta-carotene on odontogenic tumor development as a biological function rather than as a provitamin A function.

我们已经报道了在nmu处理的仓鼠门牙中诱导成牙障碍和成牙性肿瘤，并证明了维生素A对肿瘤发生和成牙障碍的影响。在本研究中，我们研究了天然和合成β -胡萝卜素对nmu处理的仓鼠的影响。在给予β -胡萝卜素6小时后，每周进行两次NMU治疗，持续3个月。nmu诱发的下颌切牙成牙障碍主要表现为牙釉质发育不全、牙釉质缺失和牙本质发育不全。在nmu处理的仓鼠中，过量的β -胡萝卜素可以轻微抑制牙生成紊乱、牙源性上皮的非典型增殖和上皮成分的角化。在NMU抑制成釉细胞分化的情况下，成釉细胞的分化和前成釉细胞与成牙细胞之间的细胞间接触未被阻止。β -胡萝卜素不能阻止nmu诱导的成釉细胞分化抑制，但能抑制nmu诱导的牙源性上皮角质化。β -胡萝卜素对牙源性肿瘤的发展有中等抑制作用。合成β -胡萝卜素似乎比天然β -胡萝卜素对nmu诱导的牙生成紊乱更有效。β -胡萝卜素可能作为一种生物功能和维生素a原功能在牙源性上皮组织的正常分化中发挥作用。这项研究可能表明β -胡萝卜素对牙源性肿瘤的抑制作用是一种生物学功能，而不是维生素a原的功能。

项目成果

Takao KOHGO et al.: "Histopathologic studies on the pothogenesis of odontogenic tumors and distwbance in odontogenesis in hamsters treated wifh N-metbylnitresourea." 北海道歯学雑誌. 15(1). 209-210 (1994)

Takao KOHGO 等人：“用 N-甲基硝基脲治疗的仓鼠牙源性肿瘤发生的组织病理学研究”15(1) (1994)。

向後隆男、Bernard T.Acob、飯塚正、進藤正信、雨宮璋: "歯原性腫瘍および歯の形成異常発生に及ぼす天然・合成β-カロテンの影響に関する病理組織学敵研究" 北海道歯学会雑誌. 17. 62-71 (1996)

Takao Kogo、Bernard T. Acob、Tadashi Iizuka、Masanobu Shindo 和 Sho Amemiya：“天然和合成 β-胡萝卜素对牙源性肿瘤和牙齿发育不良的影响的组织病理学研究”北海道牙科协会杂志 17. 62-。 71 (1996)

向後隆男: "ビタミンAとβ-カロテンの役割" 北海道歯学会雑誌. 15. 386-387 (1994)

Takao Kogo：“维生素 A 和 β-胡萝卜素的作用”北海道牙科学会杂志 15. 386-387 (1994)。

Takao Kohgo, Bernard T.Acob, Tadashi lizuka, Masanobu Shindoh and Akira Amemiya: "Histopath ologic evaluation of the effect of natural and synthetic beta-carotene on tumorigenesis and disturbances in odontogenesis in N-nitrosomethylurea-treated hamsters."

Takao Kohgo、Bernard T.Acob、Tadashi Lizuka、Masanobu Shindoh 和 Akira Amemiya：“对天然和合成 β-胡萝卜素对经 N-亚硝基甲基脲处理的仓鼠的肿瘤发生和牙发育障碍的影响进行组织病理学评估。”

向後隆男: "ビタミンAとβカロテンの役割" 北海道歯学雑誌. 15(2). 386-387 (1994)

Takao Kogo：“维生素 A 和 β-胡萝卜素的作用”北海道牙科杂志 15(2) (1994)。