Histopathological evaluation of the effects of sex hormones in DMBA-induced rat malignant salivary gland tumors with morphological transformation.

本研究采用组织病理学和免疫组织化学技术，探讨性类固醇激素是否能引起实验性dba诱导的大鼠癌的组织病理学变化。Wistar大鼠分为6组；A1)睾丸切除术后腺癌诱导组，A2)处方己烯雌酚（DES）组，B)腺癌诱导睾丸切除术后组，C1)卵巢切除术后（OV）腺癌诱导组，C2)处方DES组，D)腺癌诱导后OV组。在Ov大鼠中，腺癌的诱导明显减少，但鳞状细胞癌的发生率明显增加。Ov大鼠腺癌生长停止，肿瘤暂时消退，可见鳞状细胞癌的高比例。结果证实，dmba诱导的大鼠颌下腺腺癌似乎是性别依赖的。ER阳性率在腺癌中高于鳞状细胞癌。ER阳性在腺癌病变中的比例高于鳞状细胞癌。内质网的表达与黏液表皮样癌的细胞分化有关。兰花切除术、OV和DES注射样品显示出不同的细胞和组织学特征。在相似的组织学变化下，ck、PCNA和S-100蛋白的表达存在差异。提示内质网在恶性唾液腺肿瘤的各种形态转化中起重要作用。背景：应用免疫组织化学方法研究双管结扎致大鼠舌下腺萎缩过程中肌上皮细胞的增殖分布、腺泡细胞和导管细胞凋亡和有丝分裂的作用。腺泡细胞向导管细胞的转变和腺泡细胞的坏死在舌下腺萎缩中起重要作用。大鼠舌下腺萎缩过程中肌上皮细胞分布发生变化。少

This study examines whether the sex steroid hormone can cause histopathological changes in experimental DMBA-induced rat carcinomas, using histopathological and immunohistochemical techniques. Wistar rats were divided into 6 groups ; A1) an after orchidectomy adenocarcinoma induction group, A2) a group with prescribed diethylstilbestrol (DES), B) an after adenocarcinoma induction orchidectomy group, C1) an after ovariectomy(OV) adenocarcinoma induction group, C2) a group with prescribed DES, D) an after adenocarcinoma induction OV group.In Ov rats, induction of adenocarcinoma was significantly reduced but the incidence of squamous cell carcinomas significantly increased. Ov rats showed an arrest of growth of adenocarcinoma and a temporary regression of the tumor, consequently, high ratio of squamous cell carcinoma was seen. The results confirm that DMBA-induced adenocarcinoma in the submandibular glands of rats seemed to be sex dependent.The rate of ER positivity was larger in adenocar … More cinomatous lesions than in squamous cell carcinoma. The percentage of ER positivity was higher in the adenocarcinomatous lesions than in the squamous cell carcinomas. The expression of ER is related to cellular differentiation in mucoepidermoid-like carcinomas.The orchidectomy, OV, and DES injected samples showed various cellular and histological characteristics. There were differences in the expressions of CKs, PCNA, and S-100 protein in a similar histological changes. It is suggested that ER plays a significant role in various morphological transformations of malignant salivary gland tumors.Background : The proliferation and distribution of myoepithelial cells and the roles of apoptosis and mitosis of acinar and duct cells in the atrophy of the sublingual gland of rat induced by double duct ligation were investigated using immunohistochemistry. The transition from acinar to duct cell and the necrosis of acinar cells play important roles in the atrophy of the sublingual gland. The distribution of myoepithelial cells change during atrophy of rat sublingual glands. Less