Histopathological evaluation of the effects of sex hormones in DMBA-induced rat malignant salivary gland tumors with morphological transformation.

性激素对 DMBA 诱导的大鼠恶性唾液腺肿瘤形态转化影响的组织病理学评价。

• 批准号: 14370600
• 负责人: KOHGO Takao
• 金额: $ 6.34万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370600/
• 关键词: salivary gland tumor; morphological transformation; sex steroid hormone; orchidectomy; ovariectomy; estrogen receptor; BMBA; rat; 唾液腺腫瘍; 性ステロイドホルモン; エストロゲンレセプター; 組織像の多彩性; 実験的検索; 唾液腺萎縮; 導管結紮; 筋上皮細胞; 増殖活性; エストロジェン; 組織像の変異

This study examines whether the sex steroid hormone can cause histopathological changes in experimental DMBA-induced rat carcinomas, using histopathological and immunohistochemical techniques. Wistar rats were divided into 6 groups ; A1) an after orchidectomy adenocarcinoma induction group, A2) a group with prescribed diethylstilbestrol (DES), B) an after adenocarcinoma induction orchidectomy group, C1) an after ovariectomy(OV) adenocarcinoma induction group, C2) a group with prescribed DES, D) an after adenocarcinoma induction OV group.In Ov rats, induction of adenocarcinoma was significantly reduced but the incidence of squamous cell carcinomas significantly increased. Ov rats showed an arrest of growth of adenocarcinoma and a temporary regression of the tumor, consequently, high ratio of squamous cell carcinoma was seen. The results confirm that DMBA-induced adenocarcinoma in the submandibular glands of rats seemed to be sex dependent.The rate of ER positivity was larger in adenocar … More cinomatous lesions than in squamous cell carcinoma. The percentage of ER positivity was higher in the adenocarcinomatous lesions than in the squamous cell carcinomas. The expression of ER is related to cellular differentiation in mucoepidermoid-like carcinomas.The orchidectomy, OV, and DES injected samples showed various cellular and histological characteristics. There were differences in the expressions of CKs, PCNA, and S-100 protein in a similar histological changes. It is suggested that ER plays a significant role in various morphological transformations of malignant salivary gland tumors.Background : The proliferation and distribution of myoepithelial cells and the roles of apoptosis and mitosis of acinar and duct cells in the atrophy of the sublingual gland of rat induced by double duct ligation were investigated using immunohistochemistry. The transition from acinar to duct cell and the necrosis of acinar cells play important roles in the atrophy of the sublingual gland. The distribution of myoepithelial cells change during atrophy of rat sublingual glands. Less

本研究采用组织病理学和免疫组织化学技术，探讨性类固醇激素是否能引起实验性DMBA诱导的大鼠癌的组织病理学变化。Wistar大鼠分为6组; A1）卵巢切除术后腺癌诱导组，A2）使用己烯雌酚（DES）处方的组，B）腺癌诱导后卵巢切除术组，C1）卵巢切除术后（OV）腺癌诱导组，C2）使用DES处方的组，D）腺癌诱导后OV组。腺癌的诱导显著减少，但鳞状细胞癌的发生率显著增加。OV大鼠腺癌生长停滞，肿瘤暂时消退，因此，鳞状细胞癌的比例较高。结果表明，DMBA诱发的大鼠下颌下腺腺癌具有性别依赖性，ER阳性率在腺癌组高于腺癌组， ...更多信息 在鳞状细胞癌中，ER在腺癌中的阳性率高于鳞癌。ER的表达与粘液表皮样癌的细胞分化有关，粘液表皮样癌切除、OV和DES注射标本显示出不同的细胞和组织学特征。在相同的组织学改变中，CKs、PCNA和S-100蛋白的表达存在差异。结论：ER在涎腺恶性肿瘤的各种形态转化中起重要作用。背景：采用免疫组织化学方法研究了双侧涎腺导管结扎诱导的大鼠舌下腺萎缩过程中肌上皮细胞的增殖、分布以及腺泡和导管细胞的凋亡和有丝分裂的作用。腺泡细胞向导管细胞的转化和腺泡细胞的坏死是舌下腺萎缩的重要原因。大鼠舌下腺萎缩过程中肌上皮细胞分布的变化。少

项目成果

泉山ゆり: "舌扁平上皮がんにおけるEIAFによるMTI-MMPの発現九進と悪性形質との関連"北海道歯学雑誌. 24(2). 142-150 (2003)

Yuri Izumiyama：“EIAF 诱导的 MTI-MMP 表达与舌鳞状细胞癌恶性特征的关系”北海道牙科杂志 24（2）142-150（2003）。

Johanna Rosette K. Po: "Etsファミリーの転写因子EIAFはp53と相互作用してp21wafl/ciplの転写を活性化する"北海道歯学雑誌. 24(2). 221-228 (2003)

Johanna Rosette K. Po：“Ets 家族转录因子 EIAF 与 p53 相互作用，激活 p21wafl/cipl 转录”《北海道牙科杂志》24(2) (2003)。

Sigeru Takahashi: "Proliferation and distribution of myoepithelial cells during atrophy of the rat sublingual gland"J. Oral Pathol Med.. 32. 90-94 (2003)

Sigeru Takahashi：“大鼠舌下腺萎缩过程中肌上皮细胞的增殖和分布”J。

Akiko Takahashi: "EWS/ETS Fusions Activate Telomerase in Ewing's Tumors"CANCER RESEARCH. 63(1). 8338-8344 (2003)

Akiko Takahashi：“EWS/ETS 融合激活尤因肿瘤中的端粒酶”癌症研究。

上野尚雄: "舌扁平上皮がんにおける低酸素誘導性因子-1α(HIF1α)の発現と臨床病理学的悪性度との関連"北海道歯学雑誌. 23・2. 738-740 (2002)

Nao Ueno：“舌鳞状细胞癌中缺氧诱导因子1α（HIF1α）的表达与临床病理恶性肿瘤的关系”北海道牙科杂志23・2（2002）。