Histopathological evaluation of the effects of sex hormones in DMBA-induced rat malignant salivary gland tumors with morphological transformation.

本研究采用组织病理学和免疫组织化学技术，检验性类固醇激素是否能引起实验性DMBA诱发的大鼠癌症的组织病理学改变。将Wistar大鼠随机分为6组，A组为切除后腺癌诱导组，A2组为己烯雌酚(DES)组，B组为卵巢切除(OV)腺癌诱导组，C组为DES组，D组为卵巢切除(OV)性腺癌诱导组。OV大鼠表现为腺癌生长受阻，肿瘤暂时消退，鳞癌比例较高。结果证实，二甲基氨基丁酸诱发的大鼠颌下腺癌似乎具有性别依赖性，腺瘤…的ER阳性率较高癌性病变比鳞状细胞癌更多。腺癌组织中ER阳性百分率高于鳞癌组织。ER的表达与黏液表皮样癌的细胞分化有关。切除、卵巢和DES注射的标本显示出不同的细胞和组织学特征。在相似的组织学变化中，CKS、增殖细胞核抗原和S-100蛋白的表达存在差异。研究背景：用免疫组织化学方法研究了双管结扎诱导的大鼠舌下腺萎缩过程中肌上皮细胞的增殖和分布，以及腺泡和导管细胞的凋亡和有丝分裂在舌下腺萎缩中的作用。腺泡向导管细胞的转化和腺泡细胞的坏死在舌下腺萎缩中起重要作用。大鼠舌下腺萎缩过程中肌上皮细胞的分布变化。较少

This study examines whether the sex steroid hormone can cause histopathological changes in experimental DMBA-induced rat carcinomas, using histopathological and immunohistochemical techniques. Wistar rats were divided into 6 groups ; A1) an after orchidectomy adenocarcinoma induction group, A2) a group with prescribed diethylstilbestrol (DES), B) an after adenocarcinoma induction orchidectomy group, C1) an after ovariectomy(OV) adenocarcinoma induction group, C2) a group with prescribed DES, D) an after adenocarcinoma induction OV group.In Ov rats, induction of adenocarcinoma was significantly reduced but the incidence of squamous cell carcinomas significantly increased. Ov rats showed an arrest of growth of adenocarcinoma and a temporary regression of the tumor, consequently, high ratio of squamous cell carcinoma was seen. The results confirm that DMBA-induced adenocarcinoma in the submandibular glands of rats seemed to be sex dependent.The rate of ER positivity was larger in adenocar … More cinomatous lesions than in squamous cell carcinoma. The percentage of ER positivity was higher in the adenocarcinomatous lesions than in the squamous cell carcinomas. The expression of ER is related to cellular differentiation in mucoepidermoid-like carcinomas.The orchidectomy, OV, and DES injected samples showed various cellular and histological characteristics. There were differences in the expressions of CKs, PCNA, and S-100 protein in a similar histological changes. It is suggested that ER plays a significant role in various morphological transformations of malignant salivary gland tumors.Background : The proliferation and distribution of myoepithelial cells and the roles of apoptosis and mitosis of acinar and duct cells in the atrophy of the sublingual gland of rat induced by double duct ligation were investigated using immunohistochemistry. The transition from acinar to duct cell and the necrosis of acinar cells play important roles in the atrophy of the sublingual gland. The distribution of myoepithelial cells change during atrophy of rat sublingual glands. Less