Estrogen receptor expression and endocrine disruptors correlates with behavior and histological features of malignant epithelial tumors of the salivary gland

雌激素受体表达和内分泌干扰物与唾液腺恶性上皮肿瘤的行为和组织学特征相关

• 批准号: 12470395
• 负责人: KOHGO Takao
• 金额: $ 6.53万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470395/
• 关键词: carcinomas of salivary gland; transformation; estrogen receptor; estrogen; DMBA; salivary duct ligation; rat; clinicopathology; 唾液腺腫瘍; 分化; 増殖; 腺癌から扁平上皮癌への移行; 粘表皮癌; タモキシフェン; ヒト

1) Apoptosis and proliferation of parenchymal cells during atrophy of rat submandibular gland induced by double duct ligation were investigated. At 2 and 3 days after ligation, increased PCNA positive cells and mitoses were seen in ducts; thereafter PCNA positive cells decreased in number. At After 7 days, most acinar cells had disappeared; a few acinar cells remained, especially at the lobule periphery. Marked depletion of acinar cell by apoptosis and a concurrent short-lived cycle of duct cell proliferation were seen. Both apoptosis and proliferation of myoepithelial cells occurred, especially in the early phase of atrophy.2) We investigated a sex differences and the effects of ovariectomy on the histology of 9,10-dimethyl-1,2-benzanthracene (DMBA)-induced adenocarcinomas in the submandibular gland of rats. Wistar rats were divided four groups : (1) non ovariectomy, (2) ovariectomy(OV), (3) anti-estrogen Tamoxifen application after development of DMBA-induced adenocarcinoma (4) male … More rats. In Ov rats, induction of adenocarcinoma was significantly reduced but the incidence of squamous cell carcinomas significantly increased. Moreover, the results of anti-estrogen tamoxifen application showed similar changes as those seen in Ov rats. As a ovarian function, estrogen seemed to play an important role in the tumorigenesis and transformation of adenocarcinoma in female rat.3) Clinicopathologically, mucoepidermoid carcinomas were investigated. The sections were immunostained with ER, progesterone receptor (PgR), MIB-1, and cytokeratin (CK) 6,7,8,13, and 19 antibodies. The rate of ER positivity was larger in epidermoid cells than in intermediate cells. The percentage of ER positivity was higher in the low-grade tumors than in the high-grade ones. The MIB-1 positivity was significantly higher in high-grade than in low-grade tumors. ER expression was associated with lymph node metastasis in male mucoepidermoid carcinomas.The expression of different types of CKs and ER suggests that ER is related to cellular differentiation in mucoepidermoid carcinomas. Less

1)本文观察了双侧颌下腺导管结扎致大鼠下颌下腺萎缩过程中腺实质细胞凋亡与增殖的变化。结扎后第2、3天，胆管内PCNA阳性细胞增多，核分裂相增多，此后PCNA阳性细胞减少。7天后，大多数腺泡细胞消失，少数腺泡细胞保留，特别是在小叶周围。腺泡细胞凋亡和导管细胞增殖的同时短周期的显着消耗。肌上皮细胞既有凋亡又有增殖，尤其在萎缩早期。2）研究了9，10-二甲基-1，2-苯并蒽（DMBA）诱发的大鼠下颌下腺腺癌的性别差异及卵巢切除对腺癌组织学的影响。Wistar大鼠分为4组：（1）非卵巢切除组，（2）卵巢切除组，（3）DMBA诱发腺癌后应用抗雌激素三苯氧胺组，（4）雄性 ...更多信息 大鼠在Ov大鼠中，腺癌的诱导显著减少，但鳞状细胞癌的发生率显著增加。此外，抗雌激素他莫昔芬应用的结果显示出与Ov大鼠中观察到的变化相似的变化。雌激素作为卵巢的一种功能，在雌性大鼠腺癌的发生和转化中起重要作用。用ER、孕酮受体（PgR）、MIB-1和细胞角蛋白（CK）6、7、8、13和19抗体对切片进行免疫染色。上皮样细胞ER阳性率高于中间细胞。低级别组ER阳性率高于高级别组。MIB-1在高级别肿瘤中的阳性率显著高于低级别肿瘤。ER的表达与男性粘液表皮样癌的淋巴结转移有关，不同类型CKs和ER的表达提示ER与粘液表皮样癌的细胞分化有关。少

项目成果

S. Takahashi, S. Nakamura, R. Suzuki, N. Islam, T. Domon, T. Yamamoto, M. Wakita: "Apoptosis and mitosis of parenchymal cells in the duct - ligated rat submandibular gland"Tissue & Cell. 32(6). 457-463 (2000)

S. Takahashi、S. Nakamura、R. Suzuki、N. Islam、T. Domon、T. Yamamoto、M. Wakita：“导管中实质细胞的凋亡和有丝分裂 - 结扎大鼠颌下腺”组织

Mahfujul H.Khan: "nm23-H1 suppresses invasion of oral squamous cell carcinoma-derived cell lines without modifying matrix metalloproteinase-2 matrix metalloproteinase-9 expression"American Journal of Pathology. 158・5. 1785-1791 (2001)

Mahfujul H.Khan：“nm23-H1 抑制口腔鳞状细胞癌来源的细胞系的侵袭，而不改变基质金属蛋白酶-2 基质金属蛋白酶-9 的表达”美国病理学杂志 158・5 (2001)。

女澤史恵: "唾液腺粘表皮癌の構成細胞の動態とエストロゲンレセプターの発現"北海道歯学雑誌. 21・2. 294-302 (2000)

Fumie Onazawa：“唾液腺粘液表皮样癌的构成细胞的动态和雌激素受体的表达”北海道牙科杂志21・2（2000）。

Ken-ichi Notani, Mitsunobu Ono, Makoto Ishikawa, Tadashi, Iizuka, Takao Kohgo, Yasunori Totsuka and Hiroshi Fukuda: "Statistical Evaluation of Prognostic Factors for Adenoid Cystic Carcinoma of Salivary Gland A Study of 21 Patients Followed-up for More Th

Ken-ichi Notani、Mitsunobu Ono、Makoto Ishikawa、Tadashi、Iizuka、Takao Kohgo、Yasunori Totsuka 和 Hiroshi Fukuda：“唾液腺腺样囊性癌预后因素的统计评估对 21 名患者进行的随访研究

Yutaka Yamazaki, Itsuo Chiba, Atsuko Hirai, Yasuyuki Kaneda, Ken-ichi Notani, Hiroshi Fukuda, Haruhiko Kashiwazaki, Yasunori Totsuka, Tadashi Iizuka and Takao Kohgo: "Spacific regions (L2, L3, LSH) of p53 mutations as poor prognostic factors in oral squam

Yutaka Yamazaki、Itsuo Chiba、Atsuko Hirai、Yasuyuki Kaneda、Ken-ichi Notani、Hiroshi Fukuda、Haruhiko Kashiwazaki、Yasunori Totsuka、Tadashi Iizuka 和 Takao Kohgo：“p53 突变的空间区域（L2、L3、LSH）是不良预后因素