Estrogen receptor expression correlates with differentiation and proliferative activity of adenocarcinoma of the salivary gland

本文研究了9，10-二甲基-1，2-苯并茂(DMBA)诱导的大鼠颌下腺腺癌的性别差异和卵巢切除对其组织学的影响。Wistar大鼠，7周龄，雌雄各5只，随机分为4组：(1)不去卵巢组，(2)去卵巢组，(3)DMBA诱发的腺癌形成后应用他莫昔芬+抗雌激素药物他莫昔芬组(4只)。在32只未去卵巢的雌性大鼠中，每两周一次将0.1毫升的1%DMBA溶解于丙酮中直接注入颌下腺。21只雌性大鼠在卵巢切除(0V)后接受等量的致癌物质。每只大鼠都接受了六次注射。末次注射DMBA后4~8周处死动物。在0v大鼠中，腺癌的诱发率显著降低，而鳞癌的发生率显著增加。0V大鼠表现为腺癌生长受阻，肿瘤暂时消退，鳞癌发生率高。结果证实，DMBA诱导的大鼠颌下腺腺癌似乎具有性别依赖性。此外，抗雌激素他莫昔芬的应用结果显示与0v大鼠相似的变化，提示雌激素作为一种卵巢功能，似乎在雌性大鼠腺癌的发生中起着重要作用。本研究的目的是确定在粘液表皮样癌中是否存在雌激素受体(ER)。对19例粘液表皮样癌石蜡包埋组织进行了研究。用雌激素受体(ER)、孕激素受体(PGR)和MIB-1抗体进行免疫组织化学染色。我们证实了58%的患者ER的表达。表皮样细胞ER阳性百分率高于中间细胞。我们认为ER与粘液表皮样癌的分化有关。

We investigated a sex differences and the effects of ovariectomy on the histology of 9,10-dimethyl-1-,2-benzanthracene (DMBA)-induced adenocarcinomas in the submandibular gland of rats. Wistar rats, (7 weeks-age, 58 females and 5 males) were divided four groups : (1) non ovariectomy, (2) ovariectomy, (3) Tamoxifen application, anti-estrogen Tamoxifen application after development of DMBA-induced adenocarcinoma (4) male rats. In thirty-two females non-ovariectomized rats a 0.1 ml of 1% DMBA dissolved in acetone was injected directly into the submandibular glands biweekly. Twenty-one females rats received an equal amount of carcinogen after ovareictomy (0V). Each rats received six injections. The animals were sacrificed 4-8 weeks after last DMBA injection. In 0v rats, induction of adenocarcinoma was significantly reduced but the incidence of squamous cell carcinomas significantly increased. 0v rats showed an arrest of growth of adenocarcinoma and a temporary regression of the tumor, consequently, high ratio of squamous cell carcinoma was seen. The results confirm that DMBA-induced adenocarcinoma in the submandibular glands of rats seemed to be sex dependent.Moreover, the results of anti-estrogen tamoxifen application showed similar changes as those seen in 0v rats, which suggested that, as a ovarian function, estrogen seemed to play an important role in the tumorigenesis of adenocarcinoma in female rat.The purpose of this study is to determine whether estrogen receptor (ER) can be identified in mucoepidermoid carcinoma. Formaline-fixed, paraffin-embedded tissues from 19 patients with mucoepidermoid carcinoma were investigated. The sections were immunostained with ER, progesterone receptor (PgR) and MIB 1 antibodies. We demonstrated the expression of ER in 58% of patients. The percentage of ER positivity was larger in epidermoid cells than in intermediate cells. We consider ER to be correlated with differentiation in mucoepidermoid carcinoma.