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Estrogen receptor expression correlates with differentiation and proliferative activity of adenocarcinoma of the salivary gland

雌激素受体表达与唾液腺腺癌的分化和增殖活性相关

基本信息

批准号：
10470398
负责人：
KOHGO Takao
金额：
$ 4.93万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

We investigated a sex differences and the effects of ovariectomy on the histology of 9,10-dimethyl-1-,2-benzanthracene (DMBA)-induced adenocarcinomas in the submandibular gland of rats. Wistar rats, (7 weeks-age, 58 females and 5 males) were divided four groups : (1) non ovariectomy, (2) ovariectomy, (3) Tamoxifen application, anti-estrogen Tamoxifen application after development of DMBA-induced adenocarcinoma (4) male rats. In thirty-two females non-ovariectomized rats a 0.1 ml of 1% DMBA dissolved in acetone was injected directly into the submandibular glands biweekly. Twenty-one females rats received an equal amount of carcinogen after ovareictomy (0V). Each rats received six injections. The animals were sacrificed 4-8 weeks after last DMBA injection. In 0v rats, induction of adenocarcinoma was significantly reduced but the incidence of squamous cell carcinomas significantly increased. 0v rats showed an arrest of growth of adenocarcinoma and a temporary regression of the tumor, consequently, high ratio of squamous cell carcinoma was seen. The results confirm that DMBA-induced adenocarcinoma in the submandibular glands of rats seemed to be sex dependent.Moreover, the results of anti-estrogen tamoxifen application showed similar changes as those seen in 0v rats, which suggested that, as a ovarian function, estrogen seemed to play an important role in the tumorigenesis of adenocarcinoma in female rat.The purpose of this study is to determine whether estrogen receptor (ER) can be identified in mucoepidermoid carcinoma. Formaline-fixed, paraffin-embedded tissues from 19 patients with mucoepidermoid carcinoma were investigated. The sections were immunostained with ER, progesterone receptor (PgR) and MIB 1 antibodies. We demonstrated the expression of ER in 58% of patients. The percentage of ER positivity was larger in epidermoid cells than in intermediate cells. We consider ER to be correlated with differentiation in mucoepidermoid carcinoma.

我们研究了性别差异和卵巢切除对9，10-二甲基-1，2-苯并蒽（DMBA）诱导的大鼠下颌下腺腺癌组织学的影响。Wistar大鼠（7周龄，雌性58只，雄性5只）分为四组：（1）非卵巢切除组，（2）卵巢切除组，（3）三苯氧胺组，（4）雄鼠DMBA诱发腺癌后应用抗雌激素三苯氧胺组。在32只雌性非卵巢切除大鼠中，每两周将0.1 ml溶于丙酮的1% DMBA直接注射到下颌下腺中。21只雌性大鼠在卵巢切除术后接受等量的致癌物（0 V）。每只大鼠接受6次注射。在最后一次DMBA注射后4-8周处死动物。在0 v大鼠中，腺癌的诱导显著减少，但鳞状细胞癌的发生率显著增加。0 v大鼠的腺癌生长停止，肿瘤暂时消退，因此，鳞状细胞癌的比例很高。结果证实，DMBA诱导的大鼠下颌下腺腺癌似乎具有性别依赖性。此外，抗雌激素他莫昔芬应用的结果显示了与0 v大鼠相似的变化，这表明，作为卵巢功能，雌激素在雌性大鼠腺癌的发生中起重要作用，本研究的目的是探讨雌激素受体（ER）是否参与了腺癌的发生。可以在粘液表皮样癌中发现。研究了19例粘液表皮样癌患者的福尔马林固定、石蜡包埋组织。切片用ER、孕酮受体（PgR）和MIB 1抗体免疫染色。我们证实了ER在58%的患者中表达。上皮样细胞ER阳性率高于中间细胞。我们认为ER与粘液表皮样癌的分化有关。