A HISTOLOGICAL STUDY ON DENTAL APATITE FORMATION BY pH INDICATIVE FLUORESCECE MATERIAL

通过使用pH指示材料获得了关于牙磷灰石形成的以下结果。1.牙用磷灰石对pH指示剂（四环素）的吸附值随晶体生长而降低。pH指示材料对牙釉质蛋白和胶原蛋白的吸附值约为10%（重量比）。3个;在分光光度法中，当激发波长为380 nm时，四环素溶液的pH值（pH6.5-pH9.5）越高，其吸收值越大。4 ;吸光度值随着钙离子与四环素的比率的增加而增加，直到其达到约1：500的水平，此时值的转变停止并停留在平台上。这些结果可能有助于更好地了解牙齿硬组织。5.在牙釉质基质形成期，分泌期pH值为中性，第一成熟期pH值下降至6.0或6.2，第二成熟期pH值又回升至中性。牙釉质磷灰石可通过pH值的交互变化方式生长。

The following results were obtained concerning with dental apatite formation by using pH indicative material. 1 ; The adsorption value of pH indicative material (tetracycline) was decreased with crystal grouth of dental apatites. 2 ; The adsorption value of pH indicative material were about 10% (in weight to weight ratio) for enamel proteins and collagen. 3 ; In the spectrophotometry, the absorption value increased proportionaly in accordance with increase of pH value (pH6.5-pH9.5) of the tetracycline solution when the excitation wave length was kept at 380nm. 4 ; The absorbance value increased in accordance with increase in the ratio of calcium ion against tetracycline until it reaches of the level about 1 : 500 where the transition of the value stopped to stay on the plateau. These results may helpful to better understanding for dental hard tissue. Furthermore, it was understood that 5 ; In matrix formation stage of tooth enamel, the pH value of secretory stage was neutral, and then the pH value decreased to 6.0 or 6.2 in first maturation stage, and its value increased to neutral again in second maturation stage. The dental enamel apatites could be grown by the manner of the reciproval changing of pH values.