A HISTOLOGICAL STUDY ON DENTAL APATITE FORMATION BY pH INDICATIVE FLUORESCECE MATERIAL

用pH指示材料对牙磷灰石的形成进行了研究。1;pH指示物质（四环素）的吸附值随着牙磷灰石晶体的生长而降低。2;pH指示材料对牙釉质蛋白和胶原蛋白的吸附值约为10%（重量比）。3;在分光光度法中，当激发波长保持在380nm时，吸收值随四环素溶液pH值（ph6.5 ~ ph9.5）的增加成比例增加。吸光度值随钙离子与四环素之比的增加而增加，直到达到1:500左右的水平，吸光度值的转变停止并停留在高原上。这些结果可能有助于更好地了解牙齿硬组织。此外，据了解，5；在牙釉质基质形成阶段，分泌期pH值为中性，第一次成熟期pH值降至6.0或6.2，第二次成熟期pH值再次升高至中性。牙釉质磷灰石可以通过pH值的相互变化来生长。

The following results were obtained concerning with dental apatite formation by using pH indicative material. 1 ; The adsorption value of pH indicative material (tetracycline) was decreased with crystal grouth of dental apatites. 2 ; The adsorption value of pH indicative material were about 10% (in weight to weight ratio) for enamel proteins and collagen. 3 ; In the spectrophotometry, the absorption value increased proportionaly in accordance with increase of pH value (pH6.5-pH9.5) of the tetracycline solution when the excitation wave length was kept at 380nm. 4 ; The absorbance value increased in accordance with increase in the ratio of calcium ion against tetracycline until it reaches of the level about 1 : 500 where the transition of the value stopped to stay on the plateau. These results may helpful to better understanding for dental hard tissue. Furthermore, it was understood that 5 ; In matrix formation stage of tooth enamel, the pH value of secretory stage was neutral, and then the pH value decreased to 6.0 or 6.2 in first maturation stage, and its value increased to neutral again in second maturation stage. The dental enamel apatites could be grown by the manner of the reciproval changing of pH values.