A HISTOLOGICAL STUDY ON DENTAL APATITE FORMATION BY pH INDICATIVE FLUORESCECE MATERIAL

通过pH指示荧光材料对牙科磷灰石形成的组织学研究

• 批准号: 06671841
• 负责人: SHIMODA Shinji
• 金额: $ 1.22万
• 依托单位: TSURUMI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671841/
• 关键词: pH; FLUORESCECE; DENTAL APATITE; CALCIUM; TOOTH ENAMEL; TETRACYCLINE; Ca; 蛍光色素; 石灰化機構; 歯

The following results were obtained concerning with dental apatite formation by using pH indicative material. 1 ; The adsorption value of pH indicative material (tetracycline) was decreased with crystal grouth of dental apatites. 2 ; The adsorption value of pH indicative material were about 10% (in weight to weight ratio) for enamel proteins and collagen. 3 ; In the spectrophotometry, the absorption value increased proportionaly in accordance with increase of pH value (pH6.5-pH9.5) of the tetracycline solution when the excitation wave length was kept at 380nm. 4 ; The absorbance value increased in accordance with increase in the ratio of calcium ion against tetracycline until it reaches of the level about 1 : 500 where the transition of the value stopped to stay on the plateau. These results may helpful to better understanding for dental hard tissue. Furthermore, it was understood that 5 ; In matrix formation stage of tooth enamel, the pH value of secretory stage was neutral, and then the pH value decreased to 6.0 or 6.2 in first maturation stage, and its value increased to neutral again in second maturation stage. The dental enamel apatites could be grown by the manner of the reciproval changing of pH values.

利用pH指示剂对牙科磷灰石的形成进行了研究，得到以下结果。1.随着牙科磷灰石晶体的生长，pH指示物质(四环素)的吸附值降低。2.pH指示剂对釉质蛋白和胶原蛋白的吸附值约为10%(重量比)。3.在分光光度法中，当激发波长为380 nm时，随着四环素溶液pH值(pH 6.5~pH 9.5)的增大，吸光度呈正比增加。4.随着钙离子与四环素比例的增大，吸光度逐渐增大，直至达到1：500左右时，吸光度的变化停止并停留在平台期。这些结果可能有助于更好地了解牙体硬组织。在牙釉质基质形成阶段，牙釉质分泌期的pH值为中性，然后在第一个成熟期下降到6.0或6.2，在第二个成熟期又回升到中性。牙釉质磷灰石可以通过反复改变pH值的方式生长。

项目成果

S.SHIMODA and K.KAWASAKI: "On adsorption mode of tetracycline in hard tissue" J.Hard Tissue Biol.5(1). (1996)

S.SHIMODA 和 K.KAWASAKI：“关于四环素在硬组织中的吸附模式”J.Hard Tissue Biol.5(1)。

S.SHIMODA,K.KAWASAKI: "ON ADSORPTIONMODE OF TETRACYCLINE INHARD TISSUE" J.Hard Tissue Biol.5 (1).

S.SHIMODA，K.KAWASAKI：“四环素内部硬组织的吸附模式”J.Hard Tissue Biol.5 (1)。