Transforming growth factor-beta1 coated beta-tricalcium phosphate pellets stimulate healing of experimental bone defects of rat calvariae

为了评估TGF-β 1-涂层β-TCP颗粒移植对大鼠颅骨双侧缺损的影响，我们通过光学显微镜进行了形态学观察，使用苏木精林可辛（H-E）染色进行组织学观察，并检测碱性磷酸酶（ALP）和抗酒石酸酸性磷酸酶（TRACPase）活性用于成骨细胞和成骨细胞分布。共聚焦激光扫描显微镜（CLSM）进行新形成的骨形态计量学。H-E切片显示，在接受TGF-β 1包被的β-TCP颗粒的部位（实验部位）比单独使用β-TCP颗粒的部位（对照部位）的新骨形成更强。第4周，在实验部位观察到新的骨点：从外周和内部都发生了骨形成。TRACP酶阳性细胞和ALP酶阳性细胞在实验部位的排列频率均高于对照部位。通过计算机化CLSM像素图像分析计算的骨形成率证实了上述发现（9.3（[SY. ±.]）1.7% vs. 3.4（]SY.+-.]）第2周为0.8%，39.8（[SY. ±.]）10.9% vs. 11.8（]SY.+-.]）第4周为2.1%; TGF-β 1包被的β-TCP颗粒与β-TCP颗粒）。总之，TGF-β 1涂层β-TCP颗粒是一种推荐的促进骨形成的合成骨移植物。

In an attempt to assess the effect of TGF-beta1-coated beta-TCP pellet grafting on bilateral defects created in rat calvariae, we carried out morphological observations by light microscopy using hematoxylincosin (H-E) staining for histology and detection of alkaline phosphatase (ALPase) and tartrateresistant acid phosphatase (TRACPase) activities for ostcoblastic and osteoclastic cell distribution. Confocal laser scanning microscopy (CLSM) was performed for morphometry of newly formed bone. H-E sections disclosed stronger new bone formation in sites receiving TGF-beta1-coated beta-TCP pellets (experimental sites) than in beta-TCP pellets alone (control sites). On week 4, new bone spots in experimental sites were observed : bone formation occurred from both the periphery and within. The frequency of scquentially arranged TRACPase-positive cells and ALPase-positive cells in experimental sites was higher than in control sites. The bone formation rate calculated by computerized CLSM pixel image analysis confirmed the above findings (9.3(]SY.+-.])1.7% vs. 3.4(]SY.+-.])0.8% on week 2 and 39.8(]SY.+-.])10.9% vs. 11.8(]SY.+-.])2.1% on week 4 ; TGF-beta1-coated beta-TCP pellets vs. beta-TCP pellets). To conclude, TGF-beta1-coated beta-TCP pellet is a recommended synthetic bone graft which promotes bone formation.