A fundamental study regarding the cultured periosteum and its scaffold leading to a novel periodontal regenerative treatment

利用具有成骨特性的自体培养骨膜片进行牙周再生治疗是目前研究的热点。我们通过体外和体内实验考察了该生物片的细胞生物学特性以及各种支架材料对该生物片的反应。提示培养的骨膜片具有干细胞样细胞，并有可能通过诱导细胞分化表达成骨特性。通过在高分子或无机支架材料表面进行处理，提高了细胞的粘附性，并能支持细胞的迁移和钙化。

A novel periodontal regenerative treatment using autologous cultured periosteal sheet with an osteogenic property attracts attention nowadays.We examined the cell-biological characteristics of this sheet and the reaction of various scaffolding materials to this biological sheet through in vitro and in vivo experiments. It was suggested that cultured periosteal sheet has stem cell-like cells, and has a possibility to express osteogenic property by induction of cell differentiation. Cell adhesion was improved by making processing some treatment on the surface of high molecular or inorganic scaffolding materials, and was able to support a cell migration and calcification.