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Development of Tissue-Engineered Periodontal Regeneration Using Platelet-Rich Plasma and Periodontal Ligament Cells

利用富含血小板的血浆和牙周膜细胞开发组织工程牙周再生

基本信息

批准号：
14571979
负责人：
OKUDA Kazuhiro
金额：
$ 2.24万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

Platelet-Rich Plasma(PRP) contained high levels of PDGF and TGF-β. PRP stimulated osteoblastic DNA synthesis and cell division, but suppressed epithelial cell division. PRP also stimulated DNA synthesis in gingival fibroblasts and periodontal ligament cells. Fibrin in combination with growth factors present in PRP might effectively promote wound healing at sites of injury in periodontal tissue. As a next stage, randomized controlled clinical trial was to compare PRP+hydroxyapatite(HA), with the mixture of HA and saline in the treatment of human intrabony defects. PRP+HA led to a significantly more favorable clinical improvement. In addition to in vitro data, PRP enhanced alkaline phosphatase(ALP) activity, but neither TGF-β nor PDGF replicated this effect. As stimulation of ATP often accompanies promotion of mineralization in a variety of tissues, the ability of PRP to stimulate mineralization in PDL cell cultures was examined. PRP substantially up-regulated expression of several osteoblastic markers such as Cbfa1,BSP, and OCN, and concomitantly stimulated mineralization in PDL cells cultured on PC-coated plates. TEM findings demonstrated that mineralized spicules were deposited onto platelet aggregates but not on or within matrix vesicle-like structures. Therefore, PRP promotes in vitro mineralization both by stimulating cellular ALP activity and by providing a nucleus for mineralization in culture.Human cultured gingival epithelial sheets were used as an autografting material for regenerating gingival tissue for treatment of chronic desquamative gingivitis. Six months post-surgery in treated sites, there were gains in healthy keratinized gingiva.In the "cultured bone" project, we succeeded incorporating periodontal ligament cells to porous hydroxyapatite block by modifying the rotary culture method.

富血小板血浆（PRP）含有高水平的PDGF和TGF-β。PRP刺激成骨细胞DNA合成和细胞分裂，但抑制上皮细胞分裂。PRP还刺激牙龈成纤维细胞和牙周膜细胞的DNA合成。在PRP中存在的生长因子组合的fixin可以有效地促进牙周组织损伤部位的伤口愈合。作为下一阶段，随机对照临床试验是比较PRP+羟基磷灰石（HA）与HA和生理盐水的混合物治疗人类骨内缺损。PRP+HA导致显著更有利的临床改善。除了体外数据，PRP增强碱性磷酸酶（ALP）活性，但TGF-β和PDGF都没有复制这种效果。由于ATP的刺激通常伴随着各种组织中矿化的促进，因此检查了PRP刺激PDL细胞培养物中矿化的能力。PRP显著上调了几种成骨细胞标志物如Cbfa 1、BSP和OCN的表达，并伴随刺激了在PC包被板上培养的PDL细胞的矿化。TEM结果表明，矿化针状体沉积在血小板聚集体上，而不是在基质囊泡样结构上或内。因此，PRP通过刺激细胞ALP活性和通过在culture.Human培养的牙龈上皮片中提供矿化核来促进体外矿化，被用作用于治疗慢性脱屑性牙龈炎的牙龈组织再生的自体移植材料。在“培养骨”项目中，我们通过改进旋转培养方法，成功地将牙周韧带细胞结合到多孔羟基磷灰石块中。