Establishment of the rapid immunohistochemical staining for proliferating cell nuclear antigen in oral cancer and jaw bone.

应用免疫组化一步法--增强聚合物一步法对舌鳞癌福尔马林固定、石蜡包埋切片进行增殖细胞核抗原（PCNA）染色。采用ABC免疫组化法对EPOS-PCNA和PCNA进行比较。EPOS-PCNA标记指数（标记细胞/总细胞计数的百分比）与高压灭菌预处理的免疫组化方案之间观察到显着相关性。我们的结论是，这种方法是一种可靠的，节省时间的技术，将是有用的定量组织病理学和进行双重免疫染色。在大鼠髁突中，由于肥大软骨细胞层仍可见非特异性染色，故应尝试使用合适的封闭剂。

A rapid one-step immunostaining method, enhanced polymer one-step staining for proliferating cell nuclear antigen (EPOS-PCNA) in formalin-fixed, paraffin-embedded sections of tongue squamous cell carcinoma was investigated. EPOS-PCNA was compared with PCNA immunohistochemistry using the avidin-biotin complax (ABC) method with or without autoclave treatment. Significant correlation of PCNA labeling index (percentage of labeled cells/total cells counted) was observed between EPOS-PCNA and the immunohistochemical protocol with autoclave pretreatment. We conclude that this method is a reliable, time-sparing techniqne that would be useful in quantitative histopathology and in performing double immunostaining. In the rat mandibular condyle an appropriate blocking reagent should be tried, because we still observed non-specific staining in the layr of hypertrophic chondrocyte.