Establishment of the rapid immunohistochemical staining for proliferating cell nuclear antigen in oral cancer and jaw bone.

口腔癌和颌骨增殖细胞核抗原快速免疫组织化学染色的建立。

• 批准号: 06672008
• 负责人: SANO Kazuo
• 金额: $ 1.41万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06672008/
• 关键词: proliferating cell; oral squamous cell carcinoma; labeling index; autoclave; antigen retrieval; rat condyle; 軟骨

A rapid one-step immunostaining method, enhanced polymer one-step staining for proliferating cell nuclear antigen (EPOS-PCNA) in formalin-fixed, paraffin-embedded sections of tongue squamous cell carcinoma was investigated. EPOS-PCNA was compared with PCNA immunohistochemistry using the avidin-biotin complax (ABC) method with or without autoclave treatment. Significant correlation of PCNA labeling index (percentage of labeled cells/total cells counted) was observed between EPOS-PCNA and the immunohistochemical protocol with autoclave pretreatment. We conclude that this method is a reliable, time-sparing techniqne that would be useful in quantitative histopathology and in performing double immunostaining. In the rat mandibular condyle an appropriate blocking reagent should be tried, because we still observed non-specific staining in the layr of hypertrophic chondrocyte.

研究了一种快速一步免疫染色方法，即在福尔马林固定、石蜡包埋的舌鳞状细胞癌切片中对增殖细胞核抗原（EPOS-PCNA）进行增强聚合物一步染色。使用亲和素-生物素复合物 (ABC) 方法，在有或没有高压灭菌处理的情况下，将 EPOS-PCNA 与 PCNA 免疫组织化学进行比较。在 EPOS-PCNA 和高压灭菌预处理的免疫组织化学方案之间观察到 PCNA 标记指数（标记细胞/计数细胞总数的百分比）的显着相关性。我们的结论是，这种方法是一种可靠、省时的技术，可用于定量组织病理学和进行双重免疫染色。在大鼠下颌髁突中，应尝试适当的封闭试剂，因为我们仍然在肥大软骨细胞层中观察到非特异性染色。

项目成果

Kazuo, Sano, et al.: "Enhanced polymer one-step staining for proliferating cell nuclear antigen in the squamous cell carcinoma of the tongue." Biotechnic & Histochemistry. (in press). (1996)

Kazuo, Sano 等人：“舌鳞状细胞癌中增殖细胞核抗原的增强聚合物一步染色。”

Kazuo Sano, et al.: "Investigation of PCNA immunostaining method in oral squamous cell carcinoma." Head and Neck Cancer. 20 (2). 250 (1994)

Kazuo Sano 等人：“口腔鳞状细胞癌 PCNA 免疫染色方法的研究”。

Kazuo Sano, et al.: "PCNA immunohistochemistry in the rat mandibular condyle." Journal of Hard Tissue Biology. 3. 47-48 (1994)

Kazuo Sano 等人：“大鼠下颌髁突的 PCNA 免疫组织化学。”

Guowu Ma,Kazuo Sano et al.: "EPOS-PCNA and PCNA immunohistochemistry in the NR-S1 tisnor of the mouse compared with BrdU immunostaining" Book of Abstracts 4th International Congress on Oral Cancer. 131- (1995)

马国武、佐野一夫等人：“与 BrdU 免疫染色相比，小鼠 NR-S1 tisnor 中的 EPOS-PCNA 和 PCNA 免疫组织化学”摘要集第四届国际口腔癌大会。

佐野和生,他: "口腔扁平上皮癌におけるPCNA染色法の検討" 頭頸部腫瘍. 20. 250- (1994)

Kazuo Sano 等：“口腔鳞状细胞癌中 PCNA 染色方法的研究”头颈肿瘤。