权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Determination of alpha beta-unsaturated aldehydes formed from deteriorated deep fried oils and food in large scale cooking.

大规模烹饪过程中变质的油炸油和食品中形成的 αβ-不饱和醛的测定。

基本信息

批准号：
06680059
负责人：
FUKUDA Yasuko
金额：
$ 1.34万
依托单位：
Shizuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

To determine the amount of reactive low molecular alpha beta-unsaturated aldehydes, which were secondary formed from peroxides in autoxidation or thermal oxidation of oils and fats, a new method was investigated.As these unsaturated aldehydes were unstable, they were stabilized with carbonyl reagent, 2,4-DNPH.With the hydrazone of 4-hydroxy-2-trans-nonenal (HNE), which was the most unstable aldehyde, extracting solvent and some reactive conditions with 2,4-DNPH were examined. The extremely high recovery of hydrazone of HNE during the processes was obtained as 99.38% in preliminary HPLC examination (ODScolumn, acetonitrile/H_2O/ THF 70 : 29 : 1, detect : uv 350nm). The nine hydrazones of these alpha beta-unsaturated aldehydes were able to separate by linear gradient HPLC (ODS column, acetonitrile : H_2O : THF from 60 : 39 : lto 90 : 9 : 1 by 30min, detect : uv 350nm) and to calculate the amount of each aldehyde by heptenal, as an internal standard. The eight vegetable oils, which were purchased in some local markets, were heated in hot block bath at 200ﾟC,for 5 hours and dtermined these aldehydes by this new method. The total amout of these unsaturated aldehydes was 1200mug/ml oil in corn and safflower oils, on the other hand, 600 mug/mloil in perilla and roasted sesame oils.These results were shown that the reactive and toxic in vivo aldehydes produced from deteriorated edible oils were separated and determined with high sensitiveness by linear gradient HPLC.

为了测定油脂在自氧化或热氧化过程中产生的活性低分子α β-不饱和醛的含量，研究了用羰基试剂2，4-DNPH稳定这些不饱和醛的新方法，并以最不稳定的醛4-羟基-2-反式壬烯醛（HNE）的腙为稳定剂，用紫外分光光度法测定了它们的含量。考察了萃取溶剂及与2，4-DNPH的反应条件。在ODS柱上，乙腈/水/四氢呋喃（70：29：1），紫外检测波长350 nm，HNE的腙回收率高达99.38%。这些α-β-不饱和醛的9个腙用线性梯度HPLC（ODS柱，乙腈：水：四氢呋喃从60：39：1到90：9：1，30 min，检测波长：uv 350 nm）分离，以庚烯醛为内标计算各醛的含量。用这种新方法测定了从市场上购买的8种植物油中的醛类化合物。玉米油和红花油中不饱和醛的总量为1200 μ g/ml，紫苏油和炒芝麻油中不饱和醛的总量为600 μ g/ml，表明用线性梯度高效液相色谱法能高灵敏地分离和测定食用油变质后产生的活性和毒性醛类。