Anti-oxidative Stress Function and Utilization of Germed Sesame Seed

芝麻木脂素的健康促进作用已被阐明，近年来对木脂素的关注日益增加。本文研究了紫外分光光度法和荧光分光光度法同时测定食品中木脂素含量的方法。反负相（ODS）体系与固定正相（二氧化硅）体系的分离效果较好。探讨了口服芝麻发芽大鼠血清中木脂素含量测定的可能性，荧光检测仪检测到的芝麻素和芝麻素含量比紫外检测仪高几千倍。白芝麻（关东1号）萌发后，芝麻素和芝麻素含量降低，极性化合物含量升高，甲醇提取物中DPPH自由基清除能力和SOD活性增强。根据光谱分析，在萌发过程中含量增加的6种主要化合物中，1种被鉴定为咖啡酸糖苷酯（acteoside），其余为已知的木脂素二、三糖苷。毛蕊糖苷是一种已知的糖苷，但首次从发芽的芝麻中分离得到。比色法对DPPH自由基的清除活性只有毛蕊花苷表现出较强的清除活性。在相同摩尔浓度下，毛蕊花苷及其糖基（咖啡酸）的SOD活性明显强于trolox。

The health-promoting effect of sesame lignans has been clarified, and the concern about lignans is recently increasing. Herein the method of quantification of lignans in food using HPLC withUV spectrometer or fluorometer was examined. The separation was good with a reverse negative-phase (ODS) system in comparison with a fixed positive-phase (silica) system. The possibility of measuring lignans in the serum of the rat orally administered germinated sesame seed was examinedSesamin and sesamolin were detected thousand times higher level than UV detector by fluorescence detector.When white sesame seed (Kanto No.1) germinated, contents of sesamin and sesamolin were decreased and the contents of polar compounds were increased and DPPH radical scavenging activity and SOD activity in methanol extract were enhanced. On the basis of spectroscopic evidence, among the six main compounds whose contents increased during germination, one was identified as caffeic acid glycoside ester (acteoside) , and the others were identified as known lignan di- and tri- glucosides. Acteoside are already known glycoside but isolated from germinated sesame seed for the first time. On the DPPH radical scavenging activity by colorimetric method, only acteoside showed strong activity. On the SOD activity, acteoside and its aglycon (caffeic acid) showed stronger activity than trolox at the equal molar concentration.